1887

Abstract

SUMMARY

Two polypeptides (imm-a and imm-b) which are not induced by an immunity mutant T4Dimm2 but by a wild-type strain T4D were identified by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. Their mol. wt. were 77000 and 45000, respectively. These polypeptides exhibited a similar kinetic pattern of synthesis. Within a few minutes p.i. the primary phage established the system that inhibited gene expression of superinfecting phage. This was shown by measuring both the phenotypic expression of immunity and the synthesis of gene polypeptides. The expression of two other immediate-early genes, namely genes and 30, and the early gene 33, was not affected by primary infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-2-351
1979-11-01
2023-01-31
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/2/JV0450020351.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-2-351&mimeType=html&fmt=ahah

References

  1. Childs J. D. 1973; Superinfection exclusion by incomplete genomes of bacteriophage T4 . Journal of Virology 11:1–8
    [Google Scholar]
  2. Cornett J. B., Vallee M. 1973; The map position of the immunity (imm) gene of bacteriophage T4 . Virology 51:506–508
    [Google Scholar]
  3. Daegelen P., Brody E. 1976; Early bacteriophage T4 transcription. A diffusible product controls rIIA and rIIB RNA synthesis. Journal of Molecular Biology 103:127–142
    [Google Scholar]
  4. Dulbecco R. 1952; Mutual exclusion between related phages. Journal of Bacteriology 63:209–217
    [Google Scholar]
  5. Graham A. F. 1953; The fate of the infecting phage particle. Annales de l’InstitutPasteur 84:90–98
    [Google Scholar]
  6. Grasso R. J., Buchanan J. M. 1969; Synthesis of early RNA in bacteriophage T4-infected Escherichia coli B. Nature, London 224:882–885
    [Google Scholar]
  7. Ishibashi M., Maizel J. V. Jun 1974; The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology 57:409–424
    [Google Scholar]
  8. Jayaraman R. 1972; Transcription of bacteriophage T4 DNA by Escherichia coli RNA polymerase in vitro:identification of some immediate-early and delayed-early genes. Journal of Molecular Biology 70:253–263
    [Google Scholar]
  9. Maizel J. V. Jun 1971 In Methods in Virology vol V pp 179–246 Edited by Maramorosch K. Koprowski H. New York: Academic Press;
    [Google Scholar]
  10. Marsh R. C., Breschkin A. M., Mosig G. 1971; Origin and direction of bacteriophage T4 DNA replication. II. A gradient of marker frequencies in partially replicated T4 DNA as assayed by transformation. Journal of Molecular Biology 60:213–233
    [Google Scholar]
  11. O’farrell P. Z., Gold L. M. 1973a; Bacteriophage T4 gene expression. Evidence for two classes of prereplicativecistrons. Journal of Biological Chemistry 248:5502–5511
    [Google Scholar]
  12. O’farrell P. Z., Gold L. M. 1973b; Transcription and translation of prereplicative bacteriophage T4 genes in vitro. Journal of Biological Chemistry 248:5512–5519
    [Google Scholar]
  13. O’farrell P. Z., Gold L. M., Huang W. M. 1973; The identificationof prereplicative bacteriophage T4 proteins. Journal of Biological Chemistry 248:5499–5501
    [Google Scholar]
  14. Okamoto K. 1973; Role of T4 phage-directed protein in the establishment of resistance to T4 ghosts. Virology 56:595–603
    [Google Scholar]
  15. Okamoto K., Yutsudo M. 1974; Participation of the s gene product of phage T4 in the establishment of resistance to T4 ghosts. Virology 58:369–376
    [Google Scholar]
  16. Peterson R. F., Cohen P. S., Ennis H. L. 1972; Properties of phage T4 messenger RNA synthesized in the absence of protein synthesis. Virology 48:201–206
    [Google Scholar]
  17. Salser W., Bolle A., Epstein R. 1970; Transcription during bacteriophage T4 development: a demonstration that distinct subclasses of the ‘early’ RNA appear at different times and that some are ‘turned off’ at late times. Journal of Molecular Biology 49:271–295
    [Google Scholar]
  18. Tomizawa J., Anraku N. 1964; Molecular mechanisms of genetic recombination in bacteriophage. I. Effect of KCN on genetic recombination of phage T4 . Journal ofMolecular Biology 8:508–515
    [Google Scholar]
  19. Vallee M., Cornett J. B. 1972; A new gene of bacteriophage T4 determining immunity against super-infecting ghosts and phage in T4-infected Escherichia coli. Virology 48:777–784
    [Google Scholar]
  20. Vogel H. J., Bonner D. M. 1956; Acetylornithinaseof Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  21. Yutsudo M., Okamoto K. 1973; Immediate-early expression of the gene causing superinfection breakdown in bacteriophage T4B. Journal of Virology 12:1628–1630
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-2-351
Loading
/content/journal/jgv/10.1099/0022-1317-45-2-351
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error