Packaging of Exogenous DNA by Phage P22 Free

Abstract

SUMMARY

We describe conditions for packaging of exogenous DNA of phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in cells. The influence of several factors such as DNA- and Mg concentration and kinetics has been investigated.

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1979-11-01
2024-03-29
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