1887

Abstract

SUMMARY

We describe conditions for packaging of exogenous DNA of phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in cells. The influence of several factors such as DNA- and Mg concentration and kinetics has been investigated.

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1979-11-01
2022-08-11
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