1887

Abstract

SUMMARY

Virus-immune sera applied to infected target cells inhibited Tc cell-mediated lysis . Blocking activity was clearly present in serum by 6 days after ectromelia virus infection. Activity was found in the IgG and IgM fractions of hyperimmune sera and was specific for the immunizing virus when ectromelia and influenza viruses were used, but did not distinguish between the serologically related poxviruses ectromelia, vaccinia and rabbitpox. There was no requirement for the donors of immune serum to be of the same mouse strain as the target cells, or the same species, since rabbit sera blocked similarly to mouse sera.

These findings imply that virus-specified antigens recognized by B cells are physically close to, or identical to, the virus antigens involved in Tc cell-recognizable antigenic changes in infected cell surfaces. There was no evidence for modified H-2 molecules alone, or other proteins coded by derepressed host cell genes being recognized by virus-immune Tc cells. Significant inhibition of lysis by anti-viral antibody was only observed on fibroblast type target cells. Macrophage and P815 targets were refractory to blocking. These findings are discussed in practical terms and in relation to possible regulation of Tc cell responses by antiviral antibody.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-1-73
1979-10-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/1/JV0450010073.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-1-73&mimeType=html&fmt=ahah

References

  1. Ada G. L., Jackson P. C., Blanden R. V., Tha-Hla R., Bowern N. A. 1976; Changes in the surface of virus-infected cells recognized by cytotoxic T cells. I. Minimal requirements for lysis of ectromelia-infected P-815 cells. Scandinavian Journal of Immunology 5:23–30
    [Google Scholar]
  2. Baxter K. L. 1972; Freeze Thaw — method of preparing linear sucrose gradients. FEBS Letters ao:117–119
    [Google Scholar]
  3. Blanden R. V. 1971; Mechanisms of recovery from a generalized viral infection: mousepox. III. Regression of infectious foci. Journal of Experimental Medicine 133:1090–1109
    [Google Scholar]
  4. Blanden R. V., Ada G. L. 1978; A dual recognition model for cytotoxic T cells based on thymic selection of precursors with low affinity for self H-2 antigens. Scandinavian Journal of Immunology 7:181–190
    [Google Scholar]
  5. Blanden R. V., Dunlop M. B. C., Doherty P. C., Kohn H. I., Mckenzie I. F. C. 1976; Effects of four H-2K mutations on virus-induced antigens recognized by cytotoxic T cells. Immunogenetics 3:541–547
    [Google Scholar]
  6. Burakoff S. J., Germain R. N., Dorf M. E., Benacerraf B. 1976; Inhibition of cell-mediated cytolysis of trinitrophenyl-derivatized target cells by alloantisera directed to the products of the K and D loci of the H-2 complex. Proceedings of the National Academy of Sciences of the United States of America 73:625–629
    [Google Scholar]
  7. Doherty P. C., Blanden R. V., Zinkernagel R. M. 1976; Specificity of virus-immune effector T cells for H-2K or H-2D compatible interactions: implications for H-antigen diversity. Transplantation Reviews 29:89–124
    [Google Scholar]
  8. Downie A. W., Macdonald A. 1950; A study of the poxvirus by complement fixation and inhibition of complement-fixation methods. Journal of Pathology and Bacteriology 62:389–401
    [Google Scholar]
  9. Gardner I. D., Blanden R. V. 1976; The cell-mediated immune response to ectromelia virus infection. II. Secondary response in vitro and kinetics of memory T cell production in vivo. Cellular Immunology 22:283–296
    [Google Scholar]
  10. Gardner I. D., Bowern N. A., Blanden R. V. 1974; Cell-mediated cytotoxicity against ectromelia virus-infected target cells. I. Specificity and kinetics. European Journal of Immunology 4:63–67
    [Google Scholar]
  11. Goding J. W. 1976; Conjugation of antibodies with fluorochromes: modifications to the standard methods. Journal of Immunological Methods 13:215–226
    [Google Scholar]
  12. Hapel A., Bablanian R., Cole G. A. 1978; Inductive requirements for the generation of virus specific T lymphocytes. I. Nature of the host cell-virus interaction that triggers secondary pox virus-specific cytotoxic T lymphocyte induction. Journal of Immunology 121:736–743
    [Google Scholar]
  13. Heide K., Schwick H. G. 1973 In Handbook of Experimental Immunology chapter 6 Edited by Weir D. M. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  14. Jackson D. C., Ada G. L., Tha-Hla R. 1976; Cytotoxic T cells recognize very early, minor changes in ectromelia virus-infected target cells. Australian Journal of Experimental Biology and Medical Science 54:349–363
    [Google Scholar]
  15. Koszinowski U., Ertl H. 1976; Role of early viral surface antigens in cellular immune response to vaccinia virus. Eurpoean Journal of Immunology 6:679–683
    [Google Scholar]
  16. Langman R. E. 1978; Cell-mediated immunity and the major histocompatibility complex. Reviews of Physiology, Biochemistry and Pharmacology 81:1–34
    [Google Scholar]
  17. Pang T., Blanden R. V. 1976a; Regulation of the T cell response to ectromelia virus infection. I. Feedback suppression by effector T cells. Journal of Experimental Medicine 143:469–481
    [Google Scholar]
  18. Pang T., Blanden R. V. 1976b; The role of adherent cells in the secondary cell-mediated response in vitro to a natural poxvirus pathogen. Australian Journal of Experimental Biology and Medical Science 54:559–571
    [Google Scholar]
  19. Schmitt-Verhulst A.-M., Sachs D. H., Shearer G. M. 1976; Cell-mediated lympholysis of trinitrophenyl-modified autologous lymphocytes. Confirmation of genetic control of response to TNP-modified H-2 antigens by the use of anti-H-2 and anti-I a antibodies. Journal of Experimental Medicine 143:211–217
    [Google Scholar]
  20. Schrader J. W., Edelman G. M. 1977; Joint recognition by cytotoxic T cells of inactivated Sendai virus and products of the major histocompatibility complex. Journal of Experimental Medicine 145:523–539
    [Google Scholar]
  21. Yap K. L., Ada O. L. 1977; Cytotoxic T cells specific for influenza virus-infected target cells. Immunology 32:151–159
    [Google Scholar]
  22. Zinkernagel R. M., Doherty P. C. 1979; Virus-specific cytotoxic T cells: role of the major histocompatibility gene complex in defining specificity and responsiveness. Advances in Immunology (in the press)
    [Google Scholar]
  23. Zinkernagel R. M., Callahan G. N., Althage A., Cooper S., Streilein J. W., Klein J. 1978; The lym-phoreticular system in triggering virus-plus-self-specific cytotoxic T cells: evidence for T help. Journal of Experimental Medicine 147:897–911
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-1-73
Loading
/content/journal/jgv/10.1099/0022-1317-45-1-73
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error