1887

Abstract

SUMMARY

Complement-dependent antibody lysis of vaccinia-infected cells was examined to demonstrate the antigenic differences between extracellular (ECV) and intracellular (ICV) forms of vaccinia virus. Cytolytic antibodies present in the antisera raised against ECV or live virus (LV) were completely removed by absorption with infected cell membranes or purified ECV but not with purified ICV. Absorption with infected cell membranes also abolished the neutralizing activity of ECV and LV antisera against ECV. On the other hand, antiserum against ICV did not contain cytolytic antibodies against vaccinia-infected cells, even though its neutralizing antibody titre against ICV was high. Moreover, both ECV and ICV anti-sera neutralized a small proportion of the heterologous form of virus, despite using purified preparations of ECV and ICV, respectively, for raising these antisera in rabbits. In contrast, the Cr release test only detected the antibodies against ECV and thus can be used to differentiate between the antibody activity of a serum against ECV and ICV.

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1979-10-01
2024-04-23
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References

  1. Appleyard G., Hapel A. J., Boulter E. A. 1971; An antigenic difference between intracellular and extracellular rabbit pox virus. Journal of General Virology 13:9–17
    [Google Scholar]
  2. Boulter E. A. 1969; Protection against pox viruses. Proceedings of the Royal Society of Medicine 62:295–297
    [Google Scholar]
  3. Boulter E. A., Appleyard G. 1973; Differences between extracellular and intracellular forms of pox virus and their implications. Progress in Medical Virology 16:86–108
    [Google Scholar]
  4. Brunner K. T., Manel J., Cerottini J. C., Chapuis B. 1968; Quantitative assay of the lytic reaction of immune lymphoid cells on 51Cr labelled allogenic target cells in vitro: inhibition by iso-antibodies and drugs. Immunology 14:181–196
    [Google Scholar]
  5. Manual C. D. C. 1968; Laboratory diagnosis of viral exanthema. Course 8190C, pp 25–28 U,. S,. P,. H,. S,. Center for Disease Control; Atlanta, Georgia, U,. S,. A:
    [Google Scholar]
  6. Moller-Larsen A., Haahr S. 1978; Humoral and cell-mediated immune responses in human before and after revaccination with vaccinia virus. Infection and Immunity 19:34–39
    [Google Scholar]
  7. Notkins A. L. 1971 In Progress in Immunology x1, pp 779–786 Edited by Bernard Amos . New York: Academic Press;
    [Google Scholar]
  8. Payne L. G., Norrby E. 1976; Presence of haemagglutinin in the envelope of extracellular vaccinia virus particles. Journal of General Virology 32:63–72
    [Google Scholar]
  9. Perrin L. H., Zinkernagel R. M., Oldstone M. B. A. 1977; Immune response in humans after vaccination with vaccinia virus: generation of a virus specific cytotoxic activity by human peripheral lymphocytes. Journal of Experimental Medicine 146:949–969
    [Google Scholar]
  10. Porter D. D. 1971; Destruction of virus infected cells by immunological mechanisms. Annual Review of Microbiology 25:283–390
    [Google Scholar]
  11. Prakash J. V., Norrby E., Payne L. G. 1977; Single radial immunodiffusion test for detecting antibodies against surface antigens of intracellular and extracellular vaccinia virus. Journal of General Virology 35:463–472
    [Google Scholar]
  12. Rawls W. E., Tompkins W. A. F. 1975 In Viral Immunology and Immunopathology Vol 6: pp 99–111 Edited by Notkins A. L. New York: Academic Press;
    [Google Scholar]
  13. Roane P. R. Jun, Roizman B. 1964; Studies of the determinant antigens of viable cells. II. Demonstration of altered antigenic reactivity of Hep2 cells infected with herpes simplex virus. Virology 22:1–9
    [Google Scholar]
  14. Smith J. W., Adam E., Melnick J. L., Rawls W. E. 1972; Use of the slCr release test to demonstrate patterns of antibody response in humans to herpes simplex virus types 1 and 2. Journal of Immunology 102:554–564
    [Google Scholar]
  15. Turner G. S., Squires E. J. 1971; Inactivated small pox vaccine. Immunogenicity of inactivated intracellular and extracellular vaccinia virus. Journal of General Virology 13:19–25
    [Google Scholar]
  16. Yang J. P. S., Wentworth B. B. 1972; Study of immune cytotoxicity with herpes virus hominis infected cells. Proceedings of the Society for Experimental Biology and Medicine 141:759–764
    [Google Scholar]
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