1887

Abstract

SUMMARY

The genetic compositions of four antigenic variants of A/Memphis/1/71 (H3N2) influenza virus, which were selected by growth in the presence of monoclonal antibodies against the haemagglutinin, were compared. The results indicate that the mutant haemagglutinin genes can be differentiated by polyacrylamide gel electrophoresis of the double stranded RNA hybrids formed between virion RNA and transcripts isolated from infected cells.

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1979-10-01
2022-08-15
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References

  1. Almond J. W., Mcgeoch D., Barry R. D. 1977; Method for assigning temperature-sensitive mutations of influenza viruses to individual segments of the genome. Virology 81:62–73
    [Google Scholar]
  2. Oerhard W., Webster R. G. 1978; Antigenic drift in influenza A viruses: I. Selection and characterization of antigenic variants of A/PR/8/34 (H0N1) influenza virus with monoclonal antibodies. Journal of Experimental Medicine 148:383–392
    [Google Scholar]
  3. Hay A. J., Bellamy A. R., Abraham G., Skehel J. J., Brand C. M., Webster R. G. 1977; Procedures for characterization of the genetic material of candidate vaccine strains. International symposium on influenza immunization II, Geneva 1977. Developments in Biological Standardization vol 39: pp 15–24 Basel: S. Karger;
    [Google Scholar]
  4. Ito Y., Joklik W. K. 1972; Temperature-sensitive mutants of reovirus. II. Anomalous electrophoretic migration of certain hybrid RNA molecules composed of mutant plus strands and wild-type minus strands. Virology 50:202–208
    [Google Scholar]
  5. Kilbourne E. D. 1969; Future influenza vaccines and the use of genetic recombinants. Bulletin of the World Health Organization 41:643–645
    [Google Scholar]
  6. Laver W. G., Air G. M., Webster R. G., Gerhard W. G., Ward C. W., Dopheide T. A. A. 1979; Antigenic drift in type A influenza virus. Sequence differences in the haemagglutinin of Hong Kong (H3N2) variants selected with monoclonal hybridoma antibodies. Virology (in the press)
    [Google Scholar]
  7. Palmer D. F., Coleman M. T., Dowdle W. R., Schild G. C. 1975; Advanced laboratory techniques for influenza diagnosis. DHEW Immunology Series No. 6, pp 89–103
    [Google Scholar]
  8. Ritchey M. B., Palese P., Schulman J. L. 1976; Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein membrane protein and non-structural protein. Journal of Virology 20:307–313
    [Google Scholar]
  9. Scholtissek C., Harms E., Rohde W., Orlich M., Rott R. 1976; Correlation between RNA fragments of fowl plague virus and their corresponding gene functions. Virology 74:332–344
    [Google Scholar]
  10. Schulman J. L., Kilbourne E. D. 1969; Independent variation in nature of haemagglutinin and neuraminidase antigens of influenza virus: distinctiveness of haemagglutinin antigen of Hong Kong/68 virus. Proceedings of the National Academy of Sciences of the United States of America 63:326–333
    [Google Scholar]
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