The infectivity of cytomegalovirus (CMV), strain Davis, was inactivated by 4-nitroquinoline 1-oxide (NQO). A series of survival curves indicates that the rate of inactivation was directly dependent on the concentration of NQO over a range of 5 to 200 µg/ml. At concentrations of 1 µg/ml or less, inactivation of virus stock was not observed and at concentrations in excess of 200 µg/ml, the cellular toxicity of residual NQO prevented quantification of the relatively low surviving infectivity. At a concentration of 200 µg/ml NQO or less, the loss of virus infectivity could be clearly shown to result from the interaction of NQO with virus and not with cells, since the addition of similar doses of NQO to assay cells simultaneously with virus did not adversely affect the sensitivity of the assay cells to measure virus infectivity. Similarly, the dimethylsulphoxide carrier at concentrations of 5% or less was shown to have a negligible effect on both virus infectivity and on the sensitivity of human skin muscle cells to assay virus infectivity. NQO inactivation of virus infectivity appeared to depend very little on white light, since the kinetics of inactivation in the presence and in the absence of white light were similar.
AlbrechtT.,
RappF.1973; Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet-irradiated human cytomegalovirus. Virology 55:53–61
AlbrechtT.,
St JeorS. C.,
FunkF. C.,
RappR.1974; Multiplicity reactivation of human cytomegalo.virus inactivated by ultra-violet light. International Journal of Radiation Biology 26:445–454
EpsteinS. S.,
SmallM.,
FalkH. L.,
MantelN.1964; On the association between photodynamic and carcinogenic activities in polycyclic compounds. Cancer Research 24:855–862
GederL.,
SanfordE. J.,
RohnerT. M.,
RappF.1977; Cytomegalovirus and cancer of the prostate: in vitro transformation of human cells. Cancer Treatment Reports 61:139–146
HayashiY.1959; Histological and histochemical study of the early lesions of mouse skin after a single application of 4-nitroquinoline IV-oxide. Gann 50:219–226
IshizawaM.,
EndoH.1967; On the mode of action of a potent carcinogen, 4-hydroxyaminoquinoline 1-oxide on bacteriophage T4. Biochemical Pharmacology 16:637–646
KawazoeY.1971Recent Results in Cancer Research: Chemistry and Biological Actions of 4-Nitroquinoline 1-Oxide pp 3–16 Edited by
EndoH.,
OnoT.,
SugimuraT.
New York, Heidelberg, Berlin: Springer-Verlag;
KodamaM.,
NagataC.1967; The photosensitizing action of chemical carcinogens upon nucleic acid (in Japanese). Proceedings of the Japanese Cancer Association 26th general meeting Nagoya: p 109
LiJ. L. H.,
AlbrechtT.1979; Acquisition of a transformed phenotype by human embryo lung cells persistently infected with human cytomegalovirus following exposure to 4-nitroquinoline 1-oxide (NQO). Abstracts of the Annual Meeting of the American Society for Microbiology p 301
NagataC.1971 In Recent Results in Cancer Research: Chemistry and Biological Actions of 4-Nitroquinoline 1-Oxide pp 17–30 Edited by
EndoH.,
OnoT.,
SugimuraT.
New York, Heidelberg, Berlin: Springer-Verlag;
NagataC.,
KodamaM.,
TagashiraY.,
ImamuraA.1966; Interaction of polynuclear aromatic hydrocarbons, 4-nitroquinoline 1-oxides, and various dyes with DNA. Biopolymers 4:409–427
OkabayashiT.1953; Studies on fungistatic substances. VI. On the reaction between 4-nitroquinoline N-oxide and sulfhydril compounds (in Japanese). Yakugaku Zasshi 73:946–949
OkanoT.,
TakenakaS.,
SatoY.1968; Interaction of the carcinogen 4-nitroquinoline 1-oxide with protein and aromatic amino acids. Chemical and Pharmaceutical Bulletin (Tokyo): 16556–558
PascaA. A.,
KummerlanderL.,
PejtsikB.,
PaliK.1975; Herpesvirus antibodies and antigens in patients with cervical anaplasia and in controls. Journal of the National Cancer Institute 55:775–781
SugimuraT.,
OkabeK.,
NagaoM.1966; The metabolism of 4-nitroquinoIine-1-oxide. III. An enzyme catalyzing the conversion of 4-nitroquinoline-1-oxide to 4-hydroxyaminoquinoline-1-oxide in rat liver and hepatomas. Cancer Research 26:1717–1721
TakayamaS.,
OotaK.1961; Correlative histochemical and autoradiographical studies on the mouse skin after the painting with tritium-labeled 4-nitroquinoline N-oxide. Gann 52:321–326
WellerT. H.,
MacaulayJ. C.,
CraigJ. M.,
WirthP.1957; Isolation of intranuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proceedings of the Society for Experimental Biology and Medicine 94:4–12