Inactivation of Human Cytomegalovirus by the Chemical Carcinogen 4-Nitroquinoline 1-Oxide Free

Abstract

SUMMARY

The infectivity of cytomegalovirus (CMV), strain Davis, was inactivated by 4-nitroquinoline 1-oxide (NQO). A series of survival curves indicates that the rate of inactivation was directly dependent on the concentration of NQO over a range of 5 to 200 µg/ml. At concentrations of 1 µg/ml or less, inactivation of virus stock was not observed and at concentrations in excess of 200 µg/ml, the cellular toxicity of residual NQO prevented quantification of the relatively low surviving infectivity. At a concentration of 200 µg/ml NQO or less, the loss of virus infectivity could be clearly shown to result from the interaction of NQO with virus and not with cells, since the addition of similar doses of NQO to assay cells simultaneously with virus did not adversely affect the sensitivity of the assay cells to measure virus infectivity. Similarly, the dimethylsulphoxide carrier at concentrations of 5% or less was shown to have a negligible effect on both virus infectivity and on the sensitivity of human skin muscle cells to assay virus infectivity. NQO inactivation of virus infectivity appeared to depend very little on white light, since the kinetics of inactivation in the presence and in the absence of white light were similar.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-1-231
1979-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/1/JV0450010231.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-1-231&mimeType=html&fmt=ahah

References

  1. Albrecht T., Rapp F. 1973; Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet-irradiated human cytomegalovirus. Virology 55:53–61
    [Google Scholar]
  2. Albrecht T., St Jeor S. C., Funk F. C., Rapp R. 1974; Multiplicity reactivation of human cytomegalo.virus inactivated by ultra-violet light. International Journal of Radiation Biology 26:445–454
    [Google Scholar]
  3. Epstein S. S., Small M., Falk H. L., Mantel N. 1964; On the association between photodynamic and carcinogenic activities in polycyclic compounds. Cancer Research 24:855–862
    [Google Scholar]
  4. Geder L., Lausch R., O’Neill F., Rapp F. 1976; Oncogenic transformation of human embryo lung cells by human cytomegalovirus. Science 192:1134–1137
    [Google Scholar]
  5. Geder L., Sanford E. J., Rohner T. M., Rapp F. 1977; Cytomegalovirus and cancer of the prostate: in vitro transformation of human cells. Cancer Treatment Reports 61:139–146
    [Google Scholar]
  6. Hayashi Y. 1959; Histological and histochemical study of the early lesions of mouse skin after a single application of 4-nitroquinoline IV-oxide. Gann 50:219–226
    [Google Scholar]
  7. Huang E.-S., Roche J. K. 1978; Cytomegalovirus DNA and adenocarcinoma of the colon: evidence for latent viral infection. Lancet i:957–960
    [Google Scholar]
  8. Ishizawa M., Endo H. 1967; On the mode of action of a potent carcinogen, 4-hydroxyaminoquinoline 1-oxide on bacteriophage T4. Biochemical Pharmacology 16:637–646
    [Google Scholar]
  9. Kawazoe Y. 1971 Recent Results in Cancer Research: Chemistry and Biological Actions of 4-Nitroquinoline 1-Oxide pp 3–16 Edited by Endo H., Ono T., Sugimura T. New York, Heidelberg, Berlin: Springer-Verlag;
    [Google Scholar]
  10. Kodama M., Nagata C. 1967; The photosensitizing action of chemical carcinogens upon nucleic acid (in Japanese). Proceedings of the Japanese Cancer Association 26th general meeting Nagoya: p 109
    [Google Scholar]
  11. Li J. L. H., Albrecht T. 1979; Acquisition of a transformed phenotype by human embryo lung cells persistently infected with human cytomegalovirus following exposure to 4-nitroquinoline 1-oxide (NQO). Abstracts of the Annual Meeting of the American Society for Microbiology p 301
    [Google Scholar]
  12. Malkin M. F., Zahalsky A. C. 1966; Interaction of the water-soluble carcinogen 4-nitroquinoline N-oxide with DNA. Science 154:1665–1667
    [Google Scholar]
  13. Mufloz N. 1976; Model systems for cervical cancer. Cancer Research 36:792–793
    [Google Scholar]
  14. Nagao M., Sugimura T. 1976; Molecular biology of the carcinogen, 4-nitroquinoline 1-oxide. Advances in Cancer Research 23:131–169
    [Google Scholar]
  15. Nagata C. 1971 In Recent Results in Cancer Research: Chemistry and Biological Actions of 4-Nitroquinoline 1-Oxide pp 17–30 Edited by Endo H., Ono T., Sugimura T. New York, Heidelberg, Berlin: Springer-Verlag;
    [Google Scholar]
  16. Nagata C., Kodama M., Tagashira Y., Imamura A. 1966; Interaction of polynuclear aromatic hydrocarbons, 4-nitroquinoline 1-oxides, and various dyes with DNA. Biopolymers 4:409–427
    [Google Scholar]
  17. Nagata C., Fujii K., Epstein S. S. 1967; Photodynamic activity of 4-nitroquinoline-i-oxide and related compounds. Nature, London 215:972–973
    [Google Scholar]
  18. Okabayashi T. 1953; Studies on fungistatic substances. VI. On the reaction between 4-nitroquinoline N-oxide and sulfhydril compounds (in Japanese). Yakugaku Zasshi 73:946–949
    [Google Scholar]
  19. Okano T., Takenaka S., Sato Y. 1968; Interaction of the carcinogen 4-nitroquinoline 1-oxide with protein and aromatic amino acids. Chemical and Pharmaceutical Bulletin (Tokyo): 16556–558
    [Google Scholar]
  20. Ono T. 1964; The effect of carcinogens on the biological activity of nucleic acids (in Japanese). Tampa-kushitsu Kakusan Koso 9:1122–1128
    [Google Scholar]
  21. Pasca A. A., Kummerlander L., Pejtsik B., Pali K. 1975; Herpesvirus antibodies and antigens in patients with cervical anaplasia and in controls. Journal of the National Cancer Institute 55:775–781
    [Google Scholar]
  22. Sugimura T., Okabe K., Nagao M. 1966; The metabolism of 4-nitroquinoIine-1-oxide. III. An enzyme catalyzing the conversion of 4-nitroquinoline-1-oxide to 4-hydroxyaminoquinoline-1-oxide in rat liver and hepatomas. Cancer Research 26:1717–1721
    [Google Scholar]
  23. Takayama S., Oota K. 1961; Correlative histochemical and autoradiographical studies on the mouse skin after the painting with tritium-labeled 4-nitroquinoline N-oxide. Gann 52:321–326
    [Google Scholar]
  24. Weller T. H., Macaulay J. C., Craig J. M., Wirth P. 1957; Isolation of intranuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proceedings of the Society for Experimental Biology and Medicine 94:4–12
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-1-231
Loading
/content/journal/jgv/10.1099/0022-1317-45-1-231
Loading

Data & Media loading...

Most cited Most Cited RSS feed