Adenovirus-associated virus (AAV) structural proteins (VP1, VP2, and VP3) have been examined to determine if areas of sequence homology exist between these three virion proteins. Tryptic and chymotryptic maps have been produced which demonstrate extensive areas of sequence homology common to all three proteins. The amino acid compositions of the proteins were also determined and were found to be very similar. These data are consistent with the hypothesis that all three virion proteins arise either from a common precursor or similar transcripts.
CarterB. J.,
RoseJ. A.1974; Transcription in vivo of a defective parvovirus: sedimentation and electrophoretic analysis of RNA synthesized by adeno-associated virus and its helper adenovirus. Virology 61:182–199
FairbanksG.,
SteckT. L.,
WallachD. F. H.1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
HogganM. D.,
BlacklowN. R.,
RoweW. P.1966; Studies of small DNA viruses found in adenovirus preparations: physical, biological and immunological characteristics. Proceedings of the National Academy of Sciences of the United States of America 55:1467–1474
JohnsonF. B.,
BlacklowN. R.,
HogganM. D.1972; Immunological reactivity of antisera prepared against the sodium dodecyl sulfate-treated structural polypeptides of adenovirus-associated virus. Journal of Virology 9:1017–1026
JohnsonF. B.,
ThomsonT. A.,
TaylorP. A.,
VlasnyD. A.1977; Molecular similarities among the adenovirus-associated virus polypeptides and evidence for a precursor protein. Virology 82:1–13
LeeH. M.,
BucherD. J.,
RiordanJ. F.1978; A sensitive, inexpensive, automated microbore amino acid analyzer: analysis of viral proteins. 176th National Meeting of American Chemical Society, Biological Abstract no. 17
LeeH. M.,
FordeM. D.,
LeeM. C.,
BucherD. J.1979; Fluorometric microbore amino acid analyzer: the construction of an inexpensive, highly sensitive instrument using O-Phthalaldehyde as a detection agent. Analytical Biochemistry (in the press)
LowryO. H.,
RosebroughN. J.,
FarrA. L.,
RandallR. J.1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
MaizelJ. V.Jun1969; Acrylamide gel electrophoresis of proteins and nucleic acids. In Fundamental Techniques in Virology pp 334–362 Edited by
HabelK.,
SalzmanN. P.
New York: Academic Press;
TattersallP.,
ShatkinA. J.1977; Sequence homology between the structural polypeptides of minute virus of mice. Journal of Molecular Biology 111:375–394