The progeny from mixed infections of tobacco protoplasts with a temperature sensitive () and a temperature resistant () strain of cowpea chlorotic mottle virus at restrictive temperatures (35 °C) was largely (90%) , judged by the coat protein produced by single lesion isolates grown at 25 °C. However, although apparently at 25 °C, many isolates were temperature resistant at 35 °C and produced virus. Successive ‘purification’ through single lesions allowed normal or viruses to be recovered from these unusual isolates. The genomes of and appear able to co-exist in mixed infections; is dominant over at all temperatures so that most of the progeny has genome, but remains available to rescue under restrictive conditions. There was no evidence that the phenomenon was due to recombination.


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