1887

Journal of General Virology header logo

Volume 45, Issue 1

Comparative Studies on Marek’s Disease Virus and Herpesvirus of Turkey DNAs Free

Abstract

SUMMARY

DNA of Marek’s disease virus (MDV) was compared to that of herpes virus of turkey (HVT). Centrifugation of the two virus DNAs in neutral glycerol and CsCl density gradients showed that the MDV genome was slightly larger than that of HVT and that the buoyant density (1.705 g/ml) of MDV DNA in CsCl gradients was slightly lower than that (1.707 g/ml) of HVT DNA. MDV and HVT DNAs were digested with either RI or dIII restriction endonuclease and analysed by 0.5% agarose gel electrophoresis. The cleavage patterns of dIII or RI DNA digests of two strains of these two viruses showed general similarities between the strains, but not between MDV and HVT. However, a few fragments of RI or dIII digests of MDV DNA co-migrated with those of HVT DNA. DNA-DNA reassociation kinetics and DNA-RNA hybridization between these two viruses indicated that MDV and HVT DNAs share detectable homology, although it is less than 5%. The DNA of a HVT variant, which has lost the ability to protect chickens from Marek’s disease, appeared similar to DNA of the vaccine strain in size and buoyant density and in its restriction endonuclease cleavage pattern.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1979
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-1-119
1979-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/1/JV0450010119.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-1-119&mimeType=html&fmt=ahah

References

  1. Bachenheimer S. L., Kieff E. D., Lee L. F., Roizman B. 1972; Comparative studies of DNAs of Marek’s disease and herpes simplex viruses. In Oncogenesis and Herpesviruses pp 74–81 Edited by Biggs P. M., de The G., Payne L. N. Lyon: International Agency for Research on Cancer;
     [Google Scholar]
  2. Clements J. B., Cortini R., Wilkie N. M. 1976; Analysis of herpesvirus DNA substructure by means of restriction endonucleases. Journal of General Virology 30:243–256
     [Google Scholar]
  3. Eidson S. C., Schmittle S. C. 1968; Studies on acute Marek’s disease. I. Characteristics of isolate GA in chickens. Avian Diseases 12:467–476
     [Google Scholar]
  4. Freifelder D. 1970; Molecular weights of coliphage DNA. IV. Molecular weight of DNA from bacteriophages T4, T5, and T7 and the general problem of determination of M. Journal of Molecular Biology 54:567–577
     [Google Scholar]
  5. Frenkel N., Jacob R. J., Honess R. W., Hayward O. S., Locker H., Roizman B. 1975; Anatomy of herpes simplex virus DNA. III. Characterization of defective DNA molecules and biological properties of virus populations containing them. Journal of Virology 16:153–167
     [Google Scholar]
  6. Gubbins E. J., Newlon C. S., Kann M. D., Donelson J. E. 1977; Sequence organization and expression of a yeast plasmid DNA. Biochimica et Biophysica Acta Gene 1:185–207
     [Google Scholar]
  7. Hayward G. S., Jacob R. J., Wadsworth S. C., Roizman B. 1975; Anatomy of herpes simplex virus DNA: Evidence for four populations of molecules that differ in the orientations of their long and short components. Proceedings of the National Academy of Sciences of the United States of America 72:4243–4247
     [Google Scholar]
  8. Hirai K. 1979; The physical state of herpes simplex virus DNA in infected human cells. Microbiology and Immunology (in the press)
    [Google Scholar]
  9. Hirai K., Maeda F., Watanabe Y. 1977; Expression of early virus functions in human cytomegalovirus infected cells: effect of ultraviolet light-irradiation of the virus. Journal of General Virology 38:121–133
     [Google Scholar]
  10. Iltis J. P., Oakes J. E., Hyman R. W., Rapp F. 1977; Comparison of the DNAs of varicella-zoster viruses isolated from clinical cases of varicella and herpes zoster. Virology 82:345–352
     [Google Scholar]
  11. Kaaden O., Dietzschold B. 1972; Some characteristics of a herpesvirus of turkeys and its DNA. In Oncogenesis and Herpesviruses pp 82–87 Edited by Biggs P. M., de The G. d, Payne L. N. Lyon: International Agency for Research on Cancer;
     [Google Scholar]
  12. Kaaden O. R., Scholtz A., Ben-Zeev A., Berker Y. 1977; Isolation of Marek’s disease virus DNA from infected cells by electrophoresis on polyacrylamide gels. Archives of Virology 54:75–83
     [Google Scholar]
  13. Kaschka-Dierich CH., Bornkamm G. W., Thomssen R. 1978; No homology detectable between Marek’s disease virus (MDV) and herpesvirus of the turkey (HVT) DNA. Symposium in the E,. E,. C. programme for co-ordination of research on avian leukosis Berlin:
     [Google Scholar]
  14. Kato S., Ono K., Naito M., Doi T., Iwa N., Mori Y., Onoda T. 1970; Isolation of herpestype virus from chickens with Marek’s disease using duck embryo fibroblast cultures. Biken Journal 13:193–203
     [Google Scholar]
  15. Kieff E. D., Bachenheimer S. L., Roizman B. 1971; Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. Journal of Virology 8:125–132
     [Google Scholar]
  16. Kilpatrick B. A., Huang E., Pagano J. S. 1976; Analysis of cytomegalovirus genomes with restriction endonucleases Hind III and Eco RI. Journal of Virology 18:1095–1105
     [Google Scholar]
  17. Konobe T., Yoshida I., A Kashi M., Koyama K., Ono K., Takagi M., Takaku K., Kunlta S., Kato S. 1972; Infectivity of lyophilized herpesvirus of turkey in chickens. 73rd Meeting of the Japanese Society of Veterinary Science; Tokyo: (abstract)
    [Google Scholar]
  18. Lee L. F., Roizman B., Spear P. G., Kieff E. D., Burmester B. R., Nazerian K. 1969; Marek’s disease herpesvirus:a cytomegalovirus?. Proceedings of the National Academy of Sciences of the United States of America 64:951–956
     [Google Scholar]
  19. Lee L. F., Kieff E. D., Bachenheimer S. L., Roizman B., Spear P. G., Burmester B. R., Nazerian K. 1971; Size and composition of Marek’s disease virus deoxyribonucleic acid. Journal of Virology 7:289–294
     [Google Scholar]
  20. Lee L. F., Armstrong R. L., Nazerian K. 1972; Comparative studies of six avian herpesviruses. Avian Diseases 16:799–805
     [Google Scholar]
  21. Lee Y.-S., Yajima Y., Nonoyama M. 1977; Mechanism of infection by Epstein-Barr virus. II. Comparison of viral DNA from HR-1 and superinfected Raji cells by restriction enzymes. Virology 81:17–24
     [Google Scholar]
  22. Lee Y.-S., Tanaka A., Silver S., Smith M., Nonoyama M. 1979; Minor DNA homology between herpesvirus of turkey and Marek’s disease virus?. Virology 93:277–280
     [Google Scholar]
  23. Nazerian K., Lindahl T., Klein O., Lee L. F. 1973; Deoxyribonucleic acid of Marek’s disease virus in virus induced tumors. Journal of Virology 12:841–846
     [Google Scholar]
  24. Nonoyama M., Pagano J. S. 1972; Separation of Epstein-Barr virus DNA from large chromosomal DNA in non virus producing cells. Nature New Biology 238:169–171
     [Google Scholar]
  25. Nonoyama M., Pagano J. S. 1973; Homology between Epstein-Barr virus DNA and viral DNA from Burkitt’s lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature, London 242:44–47
     [Google Scholar]
  26. Oakes J. E., Iltis J. P., Hyman R. W., Rapp F. 1977; Analysis by restriction enzyme cleavage of human varicella-zoster virus DNAs. Virology 82:353–361
     [Google Scholar]
  27. Ono K., Doi T., Ishikawa T., Iwa N., Naito M., Kato S., Koyama K., Konobe T., Takaku K. 1974; Studies on herpesvirus of turkeys and its virological characteristics. Japanese Journal of Veterinary Science (in Japanese) 36:407–420
     [Google Scholar]
  28. Sober H. 1970 In Handbook of Biochemistry Selective Data for Molecular Biology Edited by Sober H. Cleveland, Ohio: The Chemical Rubber Co;
     [Google Scholar]
  29. Southern E. M. 1975; Detection of specific sequences and among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–512
     [Google Scholar]
  30. Studier F. W. 1965; Sedimentation studies of the size and shape of DNA. Journal of Molecular Biology 11:373–390
     [Google Scholar]
  31. Sugden B., Summers W. C., Klein G. 1976; Nucleic acid renaturation and restriction endonuclease cleavage analysis show that the DNAs of a transforming and a nontransforming strain of Epstein-Barr virus share approximately 90% of their nucleotide sequences. Journal of Virology 18:765–775
     [Google Scholar]
  32. Witter R. L. 1977; Nonprotective variants of Marek’s disease vaccine viruses. 3rd International Symposium on Oncogenesis and Herpesviruses Boston: (abstract)
    [Google Scholar]
  33. Witter R. L., Offenbecker L. 1969; Nonprotective and temperature-sensitive variants of Marek’s disease vaccine viruses. Journal of the National Cancer Institute 62:143–152
     [Google Scholar]
  34. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. 1970; Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek’s disease virus. American Journal of Veterinary Research 31:525–538
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-1-119
Loading
Comparative Studies on Marek’s Disease Virus and Herpesvirus of Turkey DNAs
J. Gen. Virol. 45, 119 (1979); https://doi.org/10.1099/0022-1317-45-1-119
/content/journal/jgv/10.1099/0022-1317-45-1-119
/content/journal/jgv/10.1099/0022-1317-45-1-119
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error