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Purified preparations of heracleum latent virus (HLV), which has very flexuous filamentous particles of about 730 × 12 nm, contain a single sedimenting component with s020,w = 96 S, A 260/A 280 = 1.50 and buoyant density in Cs2SO4 = 1.24 g/ml. The particles possess a single polypeptide, mol. wt. 23500, apparently lacking in tryptophan, and a single-stranded RNA of apparent mol. wt. 2.3 × 106 in nondenaturing conditions or 2.15 × 106 in denaturing conditions. The infectivity of the particles is sensitive to ribonuclease; this sensitivity is increased after exposure to EDTA, but is not decreased by addition of Mg2+ or Ca2+. HLV thus closely resembles apple chlorotic leaf spot virus, to which it is serologically unrelated; it is regarded as a tentative member of the closterovirus group. Its cryptogram is R/1:2.3/(5):E/E:S/Ve/Ap.
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