Mouse immune (type T) interferon was produced from suspensions of spleen cells (1 × 107 cells/ml) treated with 3 µg/ml of phytohaemagglutinin. The crude interferon was chromatographed on four sorbents with varying affinities, namely concanavalin A-Sepharose, Affi-Gel 202, Blue Sepharose CL-6B and Phenyl-Sepharose CL-4B. With each of these the interferon activity was observed to have considerable heterogeneity. By means of affinity chromatography, mouse immune interferon was purified 100 to 200 times with concomitant complete recovery of activity.
Barot-CiorbaruR.,
WietzerbinJ.,
PetitJ. F.,
ChedidL.,
FalcoffE.,
LedererE.1978; Induction of interferon synthesis in mice by fractions from Nocardia. Infection and Immunity 19:353–356
BartfeldH.,
VilcekJ.1975; Immunologically specific production of interferon in cultures of rabbit blood lymphocytes: association with in vitro tests for cell-mediated immunity. Infection and Immunity 12:1112–1115
BöhlenP.,
SteinS.,
DairmanW.,
UdenfriendS.1973; Fluorimetric assay of proteins in the nanogram range. Archives of Biochemistry and Biophysics 155:213–220
CesarioT. C.,
SchryerP.,
MandelA.,
TillesJ. G.1976; Affinity of human fibroblast interferon for blue dextran. Proceedings of the Society for Experimental Biology and Medicine 153:486–489
DaveyM. W.,
SulkowskiE.,
CarterW. A.1976a; Hydrophobic interaction of human, mouse and rabbit interferons with immobilized hydrocarbons. Journal of Biological Chemistry 251:7620–7625
DaveyM. W.,
SulkowskiE.,
CarterW. A.1976b; Purification and characterization of mouse interferon with novel affinity sorbents. Journal of Virology 17:439–445
De Maeyer-GuignardJ.,
De MaeyerE.1976; Le chromophore du Blue dextran: un ligand puissant pour la purification deTinterferon par chromatographie d’affinite. Comptes Rendus de l’Académié des Sciences, Paris 283:709–711
De Maeyer-GuignardJ.,
ThangM. N.,
De MaeyerE.1977; Binding of mouse interferon to polynucleotides. Proceedings of the National Academy of Sciences of the United States of America 74:3787–3790
De Maeyer-GuignardJ.,
ToveyM. G.,
GresserI.,
De MaeyerE.1978; Purification of mouse interferon by sequential affinity chromatography on poly(U) and antibody-agarose columns. Nature, London 271:622–625
FinterN. B.1969; Dye uptake methods for assessing viral cytopathogenicity and their application for interferon assays. Journal of General Virology 5:419–427
JankowskiW. J.,
Von MuenchhausenW.,
SulkowskiE.,
CarterW. A.1976; Binding of human interferons to immobilized Cibacron Blue F3GA; the nature of molecular interaction. Biochemistry 15:5182–5187
JohnsonH. M.,
BaronS.1976b; The nature of the suppressive effect of interferon and interferon inducers on the ‘in vitro’ immune response. Cellular Immunology 25:106–115
MizrahyA.,
O’MalleyJ. A.,
CarterW. A.,
TakatsukiA.,
TamuraG.,
SulkowskiE.1978; GlyCOSylation of interferons: Effects of tunicamycin on human immune interferon. Journal of Biological Chemistry 253:8677–8679
SonnenfeldG.,
MandelA. D.,
MeriganT. C.1977; The immunosuppressive effect of type II mouse interferon preparations on antibody production. Cellular Immunology 34:193–206
StoboJ.,
GreenI.,
JacksonL.,
BaronS.1974; Identification of a subpopulation of mouse lymphoid cells required for interferon production after stimulation with mitogens. Journal of Immunology 112:1589–1593
ValleM. J.,
BobroveA. M.,
StroberS.,
MeriganT. C.1975a; Immune specific production of interferon by human T cells in combined macrophage-lymphocyte cultures in response to herpes simplex antigen. Journal of Immunology 114:435–441
ValleM. J.,
JordanG. W.,
HaahrS.,
MeriganT. C.1975b; Characteristics of immune interferon produced by human lymphocyte cultures compared to other human interferons. Journal of Immunology 11 5:230–233
WallenC. W.,
DeanJ. H.,
LucasD. O.1973; Interferon and the cellular immune response: separation of interferon-producing cells from DNA-synthetic cells. Cellular Immunology 6:110–122
WietzerbinJ.,
FalcoffR.,
CatinotL.,
FalcoffE.1977; Affinity chromatographic analysis of murine interferons induced by viruses and by T and B cell stimulants. Annales l’lmmunologie 128 C:699–708
WietzerbinJ.,
StefanosS.,
LuceroM.,
FalcoffE.,
ThangD. C.,
ThangM. N.1978b; Presence of a polynucleotide binding site on murine immune interferon (T-type). Biochemical and Biophysical Research Communications 85:480–489
YoungnerJ. S.,
SalvinS. B.1973; Production and properties of migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. Journal of Immunology 11:1914–1922