In an organotypic nerve cell culture system, all cells in both the central and the peripheral nervous system (CNS, PNS) components supported replication of herpes simplex virus types 1 and 2 (HSV 1, HSV 2). In HSV 1 infection, cellular response was particularly characterized by the formation of small syncytia (which involved neurons) and by the presence of bundles of interwoven fine filaments within the nuclei of infected cells. In HSV 2 infection, groups of parallel tubules characteristically formed in the nuclei of infected cells. All cells in the CNS or PNS succumbed to virus infection, some within 24 h (e.g. oligodendrocytes) and others after 48 h (e.g. neurons), with the exception of astrocytes. Although among the first cells to develop virus nucleocapsids in their nuclei, astrocytes became swollen and filled with increased numbers of bundles of glial filaments within 24 h after infection; by 48 h the actual number of astrocytes was increased by as much as three- to fourfold over the number in controls. The results suggest that astrocytes may have a unique mechanism which modifies virus infection and the cells not only survive, but can also become reactive.
AtkinsonM. A.,
BarrS.,
TimburyM. C.1978; The fine structure of cells infected with temperaturesensitive mutants of herpes simplex virus type 2. Journal of General Virology 40:103–119
BornsteinM. D.1973; Organotypic mammalian central and peripheral nerve tissue. In Methods and Application of Tissue Culture pp 86–92 Edited by
KruseP. F.,
PatersonM. K.
New York: Academic Press;
CouchE. F.,
NahmiasA. J.1969; Filamentous structures of type 2 Herpesvirus hominis infection of the chorioallantoic membrane. Journal of Virology 3:228–232
Ecob-JohnstonM. S.,
ElizanT.,
SchwartzJ.,
WhetsellW. O.Jun1979; Herpes simplex virus types 1 and 2 in organotypic cultures of mouse central and peripheral nervous system. 2. Electron microscopic observations of myelin degeneration. Journal of Neuropathology & Experimental Neurology 38:10–18
FeldmanL. A.,
SheppardR. D.,
BornsteinM. D.1968; Herpes simplex virus-host cell relationships in organized cultures of mammalian nerve tissues. Journal of Virology 2:621–628
FournierJ. G.,
PrivatA.,
BouteilleM.1977; Infection of cultivated CNS tissue with herpes virus, HSV II. A reappraisal. Acta Neuropathologica 39:177–180
HillT. J.,
FieldH. J.1973; The interaction of herpes simplex virus with cultures of peripheral nervous tissue: an electron microscopic study. Journal of General Virology 21:123–133
HogganM. D.,
RoizmanB.1959; The isolation and properties of a variant of herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. American Journal of Hygiene 70:208–219
KristenssonK.,
WisniewskiH.1978; Arrest of myelination and demyelination in rabbit retina induced by herpes simplex virus infection. Neuropathology and Applied Neurobiology 4:71–82
KristenssonK.,
VahlneA.,
PerssonL. A.,
LyckeE.1978; Neural spread of herpes simplex virus types 1 and 2 in mice after corneal or subcutaneous (footpad) inoculation. Journal of the Neurological Sciences 35:331–340
LeestmaJ. E.,
BornsteinM. B.,
SheppardR. D.,
FeldmanL. A.1969; Ultrastructural aspects of herpes virus infection in organized cultures of mammalian nervous tissue. Laboratory Investigation 20:70–78
MoriR.,
MinamishimaY.,
TasakiT.,
OdaH.,
TakadeA.1973; Hexagonal arrangement of filaments in herpes simplex virus type 2-infected cells. Japanese Journal of Microbiology 17:230–232
MurphyF. A.,
HarrisonA. K.,
WhitfieldS. G.1967; Intranuclear formation of filaments in herpesvirus hominis infection in mice. Archiv für die gesamte Virus for schung 21:463–468
OdaH.,
MoriR.1976; Electron microscope observations on tubular structures in cells infected with herpes simplex virus type 2. Archives of Virology 50:159–168
SchwartzJ.,
RoizmanB.1969; Similarities and differences in the development of laboratory strains and freshly isolated strains of herpes simplex virus in HEp-2 cells: electron microscopy. Journal of Virology 4:879–889
StackpoleC. W.1969; Herpes-type virus of the frog renal adenocarcinoma. L. virus development in tumor transplants maintained at low temperatures. Journal of Virology 4:75–93
TownsendJ. J.,
BaringerJ. R.1978; Central nervous system susceptibility to herpes infection. Journal of Neuropathology & Experimental Neurology 37:255–263
WalzM. A.,
YamamotoH.,
NotkinsA. L.1976; Immunological response restricts number of cells in sensory ganglia infected with herpes simplex virus. Nature, London 264:554–556
YoungS. K.,
RoweN. H.,
SanderlinK. C.1977; Herpes simplex virus types 1 and 2 in clinical infections: differences observed by electron microscopy. Journal of Infectious Diseases 135:486–489
ZieglerR. J.,
PozosR. S.1977; Ultrastructural effects of herpes simplex virus type 2 infection of rat dorsal root ganglia in culture. Journal of Neuropathology & Experimental Neurology 36:680–692