1887

Abstract

SUMMARY

The sensitivity, reproducibility and specificity of an enzyme-linked immunosorbent assay (ELISA) for the defective phage PBS Z1 of have been investigated. It was shown that phages in concentrations between 10 and 2.5 × 10 particles/ml could be assayed with this method. The coefficient of variation for concentrations between 5 × 10 and 5 × 10 particles/ml was approx. 10%. From some other phages tested, only the defective phages resembling PBS Z1 in morphology were detected efficiently with the ELISA for PBS Z1. A comparison is made between ELISA and other assays for PBS Z1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-3-741
1979-09-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/3/JV0440030741.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-3-741&mimeType=html&fmt=ahah

References

  1. Duermeyer W., van Der Veen J., Koster B. 1978; ELISA in hepatitis A. Lancet i:823–824
    [Google Scholar]
  2. Eiserling F. A. 1964 Ph.D. thesis University of California at Los Angeles; Los Angeles:
  3. Engvall W., Perlmann P. 1971; Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871–874
    [Google Scholar]
  4. Huang W. M., Marmur J. 1970; Characterization of inducible bacteriophages in Bacillus licheniformis. Journal of Virology 5:237–246
    [Google Scholar]
  5. Nakane P. K., Kawaoi A. 1974; Peroxidase-labelled antibody. A new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  6. Okamoto K., Mudd J. A., Mangan J., Huang W. M., Subbaiah T. V., Marmur J. 1968; Properties of the defective phage of Bacillus subtilis. Journal of Molecular Biology 34:413–428
    [Google Scholar]
  7. Peterson G. L. 1977; A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83:346–356
    [Google Scholar]
  8. Schuurs A. H. W. M., van Weemen B. K. 1977; Enzyme-immunoassay. Clinica Chimica Acta 81:1–40
    [Google Scholar]
  9. Steensma H. Y., Sondermeuer P. J. A. 1977; A counting method for determining the burst size of defective phages from Bacillus subtilis. Antonie van Leeuwenhoek 43:305–316
    [Google Scholar]
  10. Steensma H. Y. 1977; The efficiency of plating (EOP) of the bacteriophages SF6, SF7, ϕ 29, PBS 1, GA-1, T3 and T4. Annali di Microbiologia ed Enzymologia 27:29–36
    [Google Scholar]
  11. Steensma H. Y., Robertson L. A., van Elsas J. D. 1978; The occurrence and taxomic value of PBS X-like defective phages in the genus Bacillus. Antonie van Leeuwenhoek 44:353–366
    [Google Scholar]
  12. Van Weemen B. K., Schuurs A. H. W. M. 1971; Immunoassay using antigen-enzyme conjugates. FEES letters 15:232–235
    [Google Scholar]
  13. Wolters G., Kuypers L., Kačaki J., Schuurs A. 1976; Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. Journal of Clinical Pathology 29:873–879
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-3-741
Loading
/content/journal/jgv/10.1099/0022-1317-44-3-741
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error