Integration of Herpes Simplex Virus Type 1 DNA into the DNA of Growth-arrested BHK-21 Cells Free

Abstract

SUMMARY

The ability of HSV-1 DNA to become associated with host cell DNA in an alkaline-stable form has been demonstrated following infection of a baby hamster kidney growth mutant ( BTN-1), at the non-permissive temperature (39.5 °C). After 8 h pre-incubation at 39.5 °C, BTN-1 cells infected at this temperature using m.o.i. ranging from 0.5 to 200 p.f.u./cell fail to replicate virus DNA even though transport of input virus genomes to the nucleus is the same at both permissive and non-permissive temperatures.

Virions containing H-labelled DNA were used to infect BTN-1 cells at 39.5 °C, and the total cellular DNA isolated from these cells was resolved into host and virus material by repeated CsCl equilibrium gradient centrifugation. A significant amount of the input radioactivity was found as a distinct band in the host region in both neutral and alkaline CsCl gradients, strongly suggesting a covalent association between host and virus DNAs. Evidence for this association was strengthened by demonstrating that radioactive material (virus DNA) banding in the host region of CsCl gradients could be driven towards the density expected for virus DNA following degradation of the putative hybrid molecules by shearing.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-3-657
1979-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/3/JV0440030657.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-3-657&mimeType=html&fmt=ahah

References

  1. Andersson-Anvret M., Lindahl T. 1978; Integrated viral DNA sequences in Epstein-Barr virus converted human lymphoma lines. Journal of Virology 25:710–718
    [Google Scholar]
  2. Babiuk L. A., Hudson J. B. 1972; ‘Integration’ of polyoma virus DNA into mammalian genomes. Biophysical and Biochemical Research Communications 47:111–118
    [Google Scholar]
  3. Bacchetti S., Graham F. L. 1977; Transfer of the gene for thymidine kinase deficient human cells by purified herpes simplex viral DNA. Proceedings of the National Academy of Sciences of the United States of America 74:1590–1594
    [Google Scholar]
  4. Biegeleisen K., Rush M. G. 1976; Association of herpes simplex virus type 1 DNA with host chromosomal DNA during productive infection. Virology 69:246–257
    [Google Scholar]
  5. Biegeleisen K., Yanagi K., Rush M. G. 1977; Further studies on the association of herpes simplex virus type 1 DNA with host DNA during productive infection. Virology 83:221–225
    [Google Scholar]
  6. Cohen S. N. 1976; Transposable genetic elements and plasmid evolution. Nature, London 263:731–738
    [Google Scholar]
  7. Darai G., Munk K. 1976; Neoplastic transformation of rat embryo cells with herpes simplex virus. International Journal of Cancer 18:469–481
    [Google Scholar]
  8. Davis D. B., Kingsbury D. T. 1976; Quantitation of the viral DNA present in cells transformed by UV-irradiated herpes simplex virus. Journal of Virology 17:788–793
    [Google Scholar]
  9. Frenkel N., Locker H., Cox B., Roizman B., Rapp F. 1976; Herpes simplex virus DNA in transformed cells: sequence complexity in five hamster cell lines and one derived hamster tumor. Journal of Virology 18:885–893
    [Google Scholar]
  10. Hayward G. S., Jacob R. J., Wadsworth S., Roizman B. F. 1975; Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components. Proceedings of the National Academy of Sciences of the United States of America 72:4243–4247
    [Google Scholar]
  11. Hirai K., Defendi V. 1972; Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of permissive monkey kidney cells. Journal of Virology 9:705–707
    [Google Scholar]
  12. Kleckner N. 1977; Translocatable elements in procaryotes. Cell n:11–23
    [Google Scholar]
  13. Kraiselburd E., Gage L. P., Weissbach A. 1975; Presence of a herpes simplex virus DNA fragment in an L cell clone obtained after infection with irradiated herpes simplex virus I. Journal of Molecular Biology 97:533–542
    [Google Scholar]
  14. Macnab J. C. M. 1974; Transformation of rat embryo cells by temperature-sensitive mutants of herpes simplex virus. Journal of General Virology 24:143–153
    [Google Scholar]
  15. Maitland N. J., McDougall J. K. 1977; Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA. Cell 11:233–241
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  17. Minson A. C., Wildy P., Buchan A., Darby G. 1978; Introduction of the herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA. Cell 13:581–587
    [Google Scholar]
  18. Munyon W., Kraiselburd E., Davis D., Mann J. 1971; Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus. Journal of Virology 7:813–820
    [Google Scholar]
  19. Nishimoto T., Basilico C. 1978; Analysis of a method for selecting temperature sensitive mutants of BHK cells. Somatic Cell Genetics 4:323–340
    [Google Scholar]
  20. Ralph R. K., Colter J. S. 1972; Evidence for the integration of polyoma virus DNA in a lytic system. Virology 48:49–58
    [Google Scholar]
  21. Rapp F., Li J. L. H. 1974; Demonstration of the oncogenic potential of herpes simplex viruses and human cytomegalovirus. Cold Spring Harbor Symposia on Quantitative Biology 39:747–763
    [Google Scholar]
  22. Schick J., Baczko K., Fanning E., Groneberg J., Burger H., Doerfler W. 1976; Intracellular forms of adenovirus DNA: integrated form of adenovirus DNA appears early in productive infection. Proceedings of the National Academy of Sciences of the United States of America 73:1043–1047
    [Google Scholar]
  23. Sheldrick P., Berthelot N. 1974; Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symposia on Quantitative Biology 39:667–678
    [Google Scholar]
  24. Turler H. 1977; Interactions of polyoma and mouse DNAs. IV. Time course and extent of integration of polyoma DNA into mouse DNA during lytic infection. Journal of Virology 23:272–285
    [Google Scholar]
  25. Tyndall C., Younghusband H. B., Bellett A. J. D. 1978; Some adenovirus DNA is associated with the DNA of permissive cells during productive or restricted growth. Journal of Virology 25:1–10
    [Google Scholar]
  26. Wigler M., Silverstein S., Lee L., Pellicer A., Chang Y., Axel R. 1977; Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell 11:223–232
    [Google Scholar]
  27. Wu G. J., Zubay G. 1974; Prolonged transcription in a cell-free system involving nuclei and cytoplasm. Proceedings of the National Academy of Sciences of the United States of America 71:1803–1807
    [Google Scholar]
  28. Yanagi K., Talavera A., Nishimoto T., Rush M. G. 1978; Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants. Journal of Virology 25:42–50
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-3-657
Loading
/content/journal/jgv/10.1099/0022-1317-44-3-657
Loading

Data & Media loading...

Most cited Most Cited RSS feed