When purified turnip yellow mosaic virus was inoculated mechanically on to Chinese cabbage leaves, using known numbers of virus particles in 0.1 to 1.0 µl volumes of inoculum, as few as 10 to 30 particles were required to produce a single local lesion. A simple procedure is described for obtaining local lesions from the virus in single infected protoplasts.
FennerF.,
McauslanB. R.,
MimsC. A.,
SambrookJ.,
WhiteD. O.1974 In The Biology of Animal Viruses second edition, pp 843 New York and London: Academic Press;
HalliwellR. S.,
GazawayW. S.1975; Quantity of microinjected tobacco mosaic virus required for infection of single cultured tobacco cells. Virology 65:583–587
KuboS.,
RobinsonD. J.,
HarrisonB. D.,
HutchesonA. M.1976; Uptake of tobacco rattle virus by tobacco protoplasts, and the effect of phosphate on infection. Journal of General Virology 30:287–298
MatthewsR. E. F.,
SarkarS.1976; A light-induced structural change in chloroplasts of Chinese cabbage infected with turnip yellow mosaic virus. Journal of General Virology 33:435–446
MotoyoshiF.,
BancroftJ. B.,
WattsJ. W.1973; A direct estimate of the number of cowpea chlorotic mottle virus particles absorbed by tobacco protoplasts that become infected. Journal of General Virology 21:159–161
TakebeL.1977; Protoplasts in the study of plant virus replication. In Comprehensive Virology vol 11 pp 237–283 Edited by
Fraenkel-ConratH.,
WagnerR. R.
New York and London: Plenum Press;
UshiyamaR.,
MatthewsR. E. F.1970; The significance of chloroplast abnormalities associated with infection by turnip yellow mosaic virus. Virology 42:293–303
WalkerH. L.,
PironeT. P.1972; Number of TMV particles required to infect locally or systemically susceptible tobacco cultivars. Journal of General Virology 17:241–243