Efficient Mechanical Inoculation of Turnip Yellow Mosaic Virus Using Small Volumes of Inoculum Free

Abstract

SUMMARY

When purified turnip yellow mosaic virus was inoculated mechanically on to Chinese cabbage leaves, using known numbers of virus particles in 0.1 to 1.0 µl volumes of inoculum, as few as 10 to 30 particles were required to produce a single local lesion. A simple procedure is described for obtaining local lesions from the virus in single infected protoplasts.

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1979-08-01
2024-03-29
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References

  1. Fenner F., Mcauslan B. R., Mims C. A., Sambrook J., White D. O. 1974 In The Biology of Animal Viruses second edition, pp 843 New York and London: Academic Press;
    [Google Scholar]
  2. Gibbs A., Harrison B. 1976 In Plant Virology. The Principles pp 292 London: Edward Arnold;
    [Google Scholar]
  3. Halliwell R. S., Gazaway W. S. 1975; Quantity of microinjected tobacco mosaic virus required for infection of single cultured tobacco cells. Virology 65:583–587
    [Google Scholar]
  4. Hibi T., Rezelman G., van kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  5. Kubo S., Robinson D. J., Harrison B. D., Hutcheson A. M. 1976; Uptake of tobacco rattle virus by tobacco protoplasts, and the effect of phosphate on infection. Journal of General Virology 30:287–298
    [Google Scholar]
  6. Luria S. E., Darnell J. E. Jun, Baltimore D., Campbell A. 1978 In General Virology third edition, pp 578 New York: Wiley;
    [Google Scholar]
  7. Matthews R. E. F. 1970 In Plant Virology pp 778 New York and London: Academic Press;
    [Google Scholar]
  8. Matthews R. E. F. 1977; Tymoviruses. In The Atlas of Insect and Plant Viruses pp 347–361 Edited by Maramorosch K. New York and London: Academic Press;
    [Google Scholar]
  9. Matthews R. E. F., Sarkar S. 1976; A light-induced structural change in chloroplasts of Chinese cabbage infected with turnip yellow mosaic virus. Journal of General Virology 33:435–446
    [Google Scholar]
  10. Motoyoshi F., Bancroft J. B., Watts J. W. 1973; A direct estimate of the number of cowpea chlorotic mottle virus particles absorbed by tobacco protoplasts that become infected. Journal of General Virology 21:159–161
    [Google Scholar]
  11. Renaudin J., Bove J. M., Otsuki Y., Takebe I. 1975; Infection of Brassica leaf protoplasts by turnip yellow mosaic virus. Virology 28:563–570
    [Google Scholar]
  12. Takebe L. 1977; Protoplasts in the study of plant virus replication. In Comprehensive Virology vol 11 pp 237–283 Edited by Fraenkel-Conrat H., Wagner R. R. New York and London: Plenum Press;
    [Google Scholar]
  13. Ushiyama R., Matthews R. E. F. 1970; The significance of chloroplast abnormalities associated with infection by turnip yellow mosaic virus. Virology 42:293–303
    [Google Scholar]
  14. Walker H. L., Pirone T. P. 1972; Number of TMV particles required to infect locally or systemically susceptible tobacco cultivars. Journal of General Virology 17:241–243
    [Google Scholar]
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