Efficient Mechanical Inoculation of Turnip Yellow Mosaic Virus Using Small Volumes of Inoculum

When purified turnip yellow mosaic virus was inoculated mechanically on to Chinese cabbage leaves, using known numbers of virus particles in 0.1 to 1.0 µl volumes of inoculum, as few as 10 to 30 particles were required to produce a single local lesion. A simple procedure is described for obtaining local lesions from the virus in single infected protoplasts.