@article{mbs:/content/journal/jgv/10.1099/0022-1317-44-2-419, author = "Maeda, Fumiko and Ihara, Seiji and Watanabe, Yasushi", title = "Morphogenesis of Nuclear Inclusions and Virus Capsids in HEL Cells Infected with Temperature-sensitive Mutants of Human Cytomegalovirus", journal= "Journal of General Virology", year = "1979", volume = "44", number = "2", pages = "419-432", doi = "https://doi.org/10.1099/0022-1317-44-2-419", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-44-2-419", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY The morphogenesis of nuclear inclusions and virus capsids in human embryonic lung cells infected with ts mutants of human cytomegalovirus at permissive (34 °C) and non-permissive (39 °C) temperatures was studied by indirect immunofluorescence (IF) and electron microscopic analyses and compared with the morphogenesis of these structures in wild-type virus infection with or without phosphonoacetate. Mutants tested belonged to five different complementation groups: two groups were DNA− (those unable to synthesize virus DNA at 39 °C) and the others were DNA+. Based on the previous finding that the electron-dense, reticular nuclear inclusions (EM-NI) observed by the thin-section analysis correspond with nuclear inclusions (IF-NI) detected by the indirect IF staining (i.e. they occupy the same space in the nucleus), the following conclusions were obtained in ts mutant infection at 39 °C: (i) the formation of EM-NI, IF-NI and virus capsids requires replication of virus DNA. (ii) The formation of EM-NI is not necessarily accompanied by the formation of IF-NI; EM-NI itself is not IF-positive unless it acquires virus-specific late antigens. (iii) The assembly of virus capsids occurs only in those cells in which EM-NI is formed; however, it can occur without the formation of IF-NI. (iv) Virus capsids assembled are not the major antigens responsible for the fluorescence of nuclear inclusions.", }