1887

Abstract

SUMMARY

A function for mitochondria in the reproduction of Rous sarcoma virus (RSV) in chronically and newly infected chick embryo cells was studied by using chloramphenicol and ethidium bromide. Chloramphenicol (CAM) and ethidium bromide (EB) were both shown to decrease the rate of growth of infected chick embryo cells and to inhibit the synthesis of mitochondrial macromolecules. Both drugs however had little or no effect on the incorporation of labelled leucine, thymidine and uridine into total cellular macromolecules. Neither CAM (80 µg/ml) nor EB (0.4 µg/ml) inhibited the production of infectious virus. In contrast, camptothecin, an inhibitor of cellular but not mitochondrial macromolecular synthesis, was shown to depress the production of infectious virus. The results indicate that the mitochondrial macromolecular synthesis machinery of RSV-infected chick embryo cells does not contribute to virus production.

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1979-08-01
2021-10-19
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References

  1. Ashwell M., Work T. S. 1970; The biogenesis of mitochondria. Annual Review of Biochemistry 39:251–290
    [Google Scholar]
  2. Avadhani N. G., Rutman R. V. 1975; Differential effects of ethidium bromide on cytoplasmic and mitochondrial protein synthesis. FEBS Letters 50:303–305
    [Google Scholar]
  3. Bader J. P. 1964; The role of deoxyribonucleic acid in the synthesis of Rous sarcoma virus. Virology 22:462–46S
    [Google Scholar]
  4. Bader A. V. 1973; Role of mitochondria in the production of RNA-containing tumor viruses. Journal of Virology 11:314–324
    [Google Scholar]
  5. Baluda M. B., Nayak D. P. 1970 In Biology of large RNA viruses chapter 3, pp 70–88 Edited by Barry R. D., Mahy B. W. J. London: Academic Press;
    [Google Scholar]
  6. Beattie D. S. 1971; The synthesis of mitochondrial proteins. Subcellular Biochemistry 1:1–23
    [Google Scholar]
  7. Bosmann H. B. 1970; Camptothecin inhibits macromole:ular synthesis in mammalian cells but not in isolated mitochondria or E. coli. Biochemical and Biophysical Research Communications 41:1412–1420
    [Google Scholar]
  8. Bosmann H. B., Myers M. W., Morgan H. R. 1974; Synthesis of DNA, RNA, protein and glycoprotein in mitochondria of cells transformed with Rous sarcoma virus. Biochemical and Biophysical Research Communications 56:75–83
    [Google Scholar]
  9. Dimitriadis G. J., Georgatsos J. G. 1974; Induction of protein synthesis in mitochondria by exogenous RNA. Synthesis of rabbit globin, by isolated mitochondria from Tetrahymena pyriformis. FEBS Letters 46:96–100
    [Google Scholar]
  10. Duesberg P. H., Vogt P. K. 1969; On the role of DNA synthesis in avian tumor virus infection. Proceedings of the National Academy of Sciences of the United States of America 64:939–946
    [Google Scholar]
  11. Ebner E., Mennucci L., Schatz G. 1973; Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. Journal of Biological Chemistry 248:5360–5368
    [Google Scholar]
  12. Fettes I. M., Holdar D., Freedman K. B. 1972; Effects of chloramphenicol on enzyme synthesis and growth of mammalian cells. Canadian Journal of Biochemistry 50:200–209
    [Google Scholar]
  13. Gaitskhoki V. S., Yershov F. I., Kisselev O. I., Zaitseva O. V., Zhdanov U. M., Neifakh S. A. 1975; On the synthesis of viral ribonucleic acids and ribonucleoproteins in the submitochondrial system completely free of interfering cytoplasmic contamination. Molecular and Cellular Biochemistry 10:17–26
    [Google Scholar]
  14. Gazzolo L., De The G., Vigier P., Sarma P. S. 1969; Presence de particules a l’aspect de nucleocapsides associees aux mitochondries dans des cellules de hamster transformees par le virus de Rous. Comptes Rendus hebdomadaire des seances de VAcademic des Sciences 268:1668–1670
    [Google Scholar]
  15. Grivell L. A., Metz V. 1973; Inhibition by ethidium bromide of mitochondrial protein synthesis programmed by imported poly(U). Biochemical and Biophysical Research Communications 55:125–131
    [Google Scholar]
  16. Guntaka R. V., Mahy B. W. J., Bishop J. M., Varmus H. E. 1975; Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virus. Nature, London 253:507–511
    [Google Scholar]
  17. Haguenau F., Beard J. W. 1962 In Tumour Induced by Virus: Ultrastructural Studies vol I chapter I, pp 1–59 Edited by Dalton A. J., Haguenau F. New York: Academic Press;
    [Google Scholar]
  18. Kara J., Mach O., Cerna H. 1971; Replication of Rous sarcoma virus and the biosynthesis of the oncogenic subviral ribonucleoprotein particles (‘virosomes’) in the mitochondria isolated from Rous sarcoma tissue. Biochemical and Biophysical Research Communications 44:162–170
    [Google Scholar]
  19. Kara J., Cerna H., Dvorak M. 1977; Biosynthesis of subviral oncogenic particles (virosomes) in mitochondria of Rous sarcoma and Rauscher murine leukemia cells. Molecular and Cellular Biochemistry 14:135–142
    [Google Scholar]
  20. Kroon A. M. 1965; Protein synthesis in mitochondria. III. On the effects of inhibitors on the incorporation of amino acids into proteins by intact mitochondria and digitonin fractions. Biochimica et Biophysica Acta 108:275–284
    [Google Scholar]
  21. Leblond-Larouche L., Morais R. 1976a; Long-term cultures of chick embryo fibroblasts transformed by the Schmidt-Ruppin strain (D) of Rous sarcoma virus. Canadian Journal of Microbiology 22:1474–1479
    [Google Scholar]
  22. Leblond-Larouche L., Morais R. 1976b; Poly(A) hydrolase of chick embryo fibroblasts. European Journal of Biochemistry 65:423–430
    [Google Scholar]
  23. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  24. Lunger P. D., Clark H. F. 1973; Ultrastructural studies of cell-virus interaction in reptilian cell lines. I. Intramitochondrial viruses in ‘C-type’ virus-producing VSW cells. Journal of the National Cancer Institute 50:111–117
    [Google Scholar]
  25. Lunger P. D., Kleitmann W., Clark H. F. 1977; Ultrastructural studies of cell-virus interaction in reptilian cell lines. IV. Effects of chloramphenicol and ethidium bromide on VSW cell mitochondria and associated virions. Acta Virologica 21:375–382
    [Google Scholar]
  26. Mach O., Kara J. 1971; Presence of Rous sarcoma virus inside the mitochondria isolated by zonal and differential centrifugation from Rous sarcoma cells. Folia Biologica (Praha) 17:65–72
    [Google Scholar]
  27. Nass M. M. K. 1972; Differential effects of ethidium bromide on mitochondrial and nuclear DNA synthesis in vivo on cultured mammalian cells. Experimental Cell Research 72:211–222
    [Google Scholar]
  28. Nass M. M. K. 1977; Studies on the synthesis and structure of mitochondrial DNA in cells infected by Rous sarcoma viruses and on the occurrence of intramitochondrial virus-like particles in certain RSV-induced tumor cells. Molecular and Cellular Biochemistry 14:121–128
    [Google Scholar]
  29. Radsack K., Kato R., Sato N., Koprowski H. 1971; Effects of ethidium bromide on mitochondrial DNA and cytochrome synthesis in HeLa cells. Experimental Cell Research 66:410–416
    [Google Scholar]
  30. Richert N. I., Hare J. D. 1972; Distinctive effects of inhibitors of mitochondrial function on Rous sarcoma virus replication and malignant transformation. Biochemical and Biophysical Research Communications 46:5–10
    [Google Scholar]
  31. Roa R. C., Bose S. K. 1974; Inhibition by ethidium bromide of the establishment of infection by murine sarcoma virus. Journal of General Virology 25:197–205
    [Google Scholar]
  32. Shanmugan G. 1976; Endonucleolytic cleavage of murine leukemia virus 35S RNA by microsome-associated nuclease. Biochemical and Biophysical Research Communications 70:818–823
    [Google Scholar]
  33. Sottocosa G. L., Kaylenstierna B., Ernster L., Bergstrand A. 1967; An electron transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study. Journal of Cell Biology 32:415–438
    [Google Scholar]
  34. Storrie B., Attardi G. 1972; Expression of the mitochondrial genome in HeLa cells. XIII. Effect of the selective inhibition of cytoplasmic or mitochondrial protein synthesis on mitochondrial nucleic acid synthesis. Journal of Molecular Biology 71:177–199
    [Google Scholar]
  35. Temin H. M. 1964; The participation of DNA in Rous sarcoma virus production. Virology 23:486–494
    [Google Scholar]
  36. Varmus H. E., Guntaka R. V., Fan W. J. W., Heasley S., Bishop J. M. 1974; Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 71:3874–3878
    [Google Scholar]
  37. Vigier P., Golde A. 1964; Etude du systeme virus de Rous-cellules d’embryon de poule h l’aide d’analogues et d’antibiotiqucs. Bulletin du Cancer 51:73–81
    [Google Scholar]
  38. Vogt P. K. 1969 In Fundamental Techniques in Virology chapter 18, pp 198–211 Edited by Habel K., Salzman N. B. New York: Academic Press;
    [Google Scholar]
  39. Wallace R. B., Williams T. M., Freeman K. B. 1975; Mitochondrial protein synthesis in a mammalian cell-line with a temperature-sensitive leucyl-tRNA synthetase. European Journal of Biochemistry 59:168–173
    [Google Scholar]
  40. Zylber E., Vesco C., Penman S. 1969; Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromide. Journal of Molecular Biology 44:195–204
    [Google Scholar]
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