Mouse Hybrid Cell Lines produce Antibodies to Herpes Simplex Virus Type 1 Free

Abstract

SUMMARY

A solid-phase radioimmunoassay procedure has been devised for the assay of antibodies produced in the mouse to herpes simplex virus type 1 (HSV-1). It is based on the adsorption of virus to flexible micro-well plates and uses radio-iodine-labelled rabbit antibody against mouse immunoglobulin to assess antibody binding. Using this assay for screening, cell hybrids have been obtained which yield monoclonal antibody to HSV-1. The hybrids are between spleen cells from hyperimmune mice and an immunoglobulin-non-secreting, azaguanine resistant myeloma cell line (NS-1). From 480 hybrid cell lines initially examined, five stable cell lines were obtained which released HSV-1-specific antibody and . Mice carrying transplants of these cell lines yield binding titres in serum of up to 1/25000. Both IgG and IgM antibodies were obtained in this way.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-1-81
1979-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/1/JV0440010081.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-1-81&mimeType=html&fmt=ahah

References

  1. Aurelian L., Strnad B. C. 1976; Herpes virus type 2 related antigens and their relevance to humoural and cell mediated immunity in patients with cervical cancer. Cancer Research 36:810–820
    [Google Scholar]
  2. Barnstable C. J., Bodmer W. F., Braun G., Galfre G., Milstein C., Williams A. F., Ziegler A. 1978; Production of monoclonal antibodies to group A erythrocytes, HLA, and other human cell surface antigens - new tools for genetic analysis. Cell 14:9–20
    [Google Scholar]
  3. Della-Porta A. J., Westawav E. G. 1978; A multi-hit model for the neutralisation of animal viruses (review article). Journal of General Virology 38:1–19
    [Google Scholar]
  4. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. 1977; Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266:550–552
    [Google Scholar]
  5. Glorioso J. C., Smith J. W. 1977; Immune interactions with cells infected with herpes simplex virus: antibodies to radio-iodinated surface antigens. Journal of Immunology 118:114–121
    [Google Scholar]
  6. Hampar B., Notkins A. L., Mage M., Keehn M. A. 1968; Heterogeneity in the properties of 7s and 19s rabbit neutralising antibodies to herpes simplex virus. Journal of Immunology 100:586–593
    [Google Scholar]
  7. Heine J. W., Spear P. G., Roizman B. 1972; Proteins specified by Herpes simplex virus. VI. Viral proteins in the plasma membrane. Journal of Virology 9:431–439
    [Google Scholar]
  8. Honess R. W., Watson D. H. 1974; Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates. Journal of General Virology 22:171–185
    [Google Scholar]
  9. Hunter W. M., Greenwood F. C. 1962; Preparation of 131iodine-labelled human growth hormone of high specific activity. Nature, London 194:495–496
    [Google Scholar]
  10. Kalino K. O. K., Ziola B. R., Vilganen M. K., Gransfors K., Toivanen P. 1977a; Solid-phase radioimmunoassay of herpes simplex IgG and IgM antibodies. Journal of Immunological Methods 14:183–195
    [Google Scholar]
  11. Kalino K. O. K., Martittila R. J., Gransfors K., Vilganen M. K. 1977b; Solid phase radioimmunoassay of human immunoglobulin M and immunoglobulin G antibodies against herpes simplex type-1 capsid, envelope and excreted antigens. Infection and Immunity 15:883–889
    [Google Scholar]
  12. Kohler G., Milstein C. 1976; Derivation of specific antibody-producing tissue culture and tumour lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  13. Koprowski H., Gerhard W., Croce C. M. 1977; Production of antibodies against influenza virus by somatic cell hybrids between mouse melanoma and primed spleen cells. Proceedings of the National Academy of Sciences of the United States of America 74:2985–2988
    [Google Scholar]
  14. Koprowski H., Gerhard W., Wiktor T., Martinis J., Shander M., Croce C. M. 1978; Anti-viral and antitumour antibodies produced by somatic cell hybrids. In Lymphocyte Hybridomas pp 8–19 Edited by Melchers F., Potter M., Warner N. L. New York: Springer-Verlag;
    [Google Scholar]
  15. Littlefield J. W. 1964; Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145:709–710
    [Google Scholar]
  16. Melchers F., Potter M., Warner N. L. 1978; Lymphocyte hybridomas. In Contemporary Topics in Microbiology and Immunology vol 81246 pp New York: Springer-Verlag;
    [Google Scholar]
  17. Miyamoto K., Morgan C., Hsu K. C., Hampar B. 1971; Differentiation by immunoferritin of herpes simplex virion antigens with the use of rabbit 7s and 19s antibodies from early (7-day) and late (7-week) immune sera. Journal of the National Cancer Institute 46:629–637
    [Google Scholar]
  18. Porath J., Axen R., Ernback S. 1967; Chemical coupling of proteins to agarose. Nature, London 215:1491–1493
    [Google Scholar]
  19. Powell K. L., Buchan A., Sim C., Watson D. H. 1974; Type specific protein in herpes simplex virus envelope reacts with neutralising antibody. Nature, London 249:360–361
    [Google Scholar]
  20. Raff M. C., Megson M., Owen J. J. T., Cooper M. D. 1976; Early production of intracellular IgM by B lymphocyte precursors in mouse. Nature, London 259:224–226
    [Google Scholar]
  21. Rosenthal J. D., Hayashi K., Notkins A. L. 1973; Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and anti-viral antibody. Applied Microbiology 25:567–573
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-1-81
Loading
/content/journal/jgv/10.1099/0022-1317-44-1-81
Loading

Data & Media loading...

Most cited Most Cited RSS feed