The human lymphoblastoid cell line, Namalwa, can be cultured in serum-free media to cell densities of 3 to 4 × 106 cells per ml. These cultures produce up to 10000 units of interferon per ml when induced with Newcastle disease virus, strain B1. Maximum accumulation of interferon was obtained at approx. 13 h post-induction.
BuhlS. N.,
ReganJ. D.1972; Growth of a human leukemia cell line on protein-free medium. Proceedings of the Society for Experimental Biology and Medicine 140:1224–1227
HoroszewiczJ. S.,
LeongS. S.,
ItoM.,
Di berardinoL.,
CarterW. A.1978; Aging in vitro and large scale interferon production by 15 new strains of human diploid fibroblasts. Infection and Immunity 19:720–726
MizrahiA.,
BinoT.,
ReuvenyS.,
TraubA.1977The 5th Aharon Katzir-Katchalsky Conference: symposium on interferons and the control of cell-virus interactions Rehovot, Israel: May 2–6 p 20
TakaokaT.,
KatsutaH.1971; Long-term cultivation of mammalian cell strains in protein and lipid-free chemically defined synthetic media. Experimental Cell Research 67:295–304
ZoonK. C.,
BucklerC. E.,
BridgenP. J.,
Gurari-rotmanD.1978; Production of human lymphoblastoid interferon by Namalwa cells. Journal of Clinical Microbiology 7:44–51