1887

Abstract

SUMMARY

Guinea pigs immunized with polypeptides derived from plantar and hand wart viruses developed both humoral and cell mediated immunity. The specificity of the antiserum was determined by indirect immunofluorescence (IF) tests and cellular immunity by intradermal tests. While whole plantar and hand wart virus particles (PV and HV) appeared to be immunologically analogous, they had different polypeptide patterns as shown by analysis on acrylamide slab gels. In particular, the P2 polypeptide (major virus protein) with different mol. wt. for PV (56750) and HV (54500) induced the production of high antibody titres in the animals and the immune sera specifically labelled wart substrates as shown in the IF test, demonstrating that no cross humoral reaction occurs between these two polypeptides. Furthermore, a delayed hypersensitivity reaction was observed in P2 polypeptide-inoculated guinea pigs when whole particles were introduced in skin tests, but a total cross reactivity between PV and HV was noticed at the cellular level. However, the study of the virus isolated from the lesions of a patient (Ri) bearing extensive common hand warts has shown that the virus particles possessed all the biochemical and immunological characteristics of PV, in particular with regard to the P2 polypeptide. Such a case may represent plantar-like warts located on the hands.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-3-713
1979-06-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/3/JV0430030713.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-3-713&mimeType=html&fmt=ahah

References

  1. Favre M., Ohrt G., Croissant O., Yaniv M. 1977; Human papilloma virus DNA: physical mapping of the cleavage sites of bacillus amyloliquefaciens (Bam 1) and haemophilus parainfluenzae (MpaII) endonucleases and evidence for partial heterogeneity. Journal of Virology 21:1210–1214
    [Google Scholar]
  2. Gissmann L., Zur Hausen H. 1976; Human papilloma virus DNA: physical mapping and genetic heterogeneity. Proceedings of the National Academy of Sciences of the United States of America 73:1310–1313
    [Google Scholar]
  3. Gissmann L., Pfister H., Zur Hausen H. 1977; Human papilloma viruses (HPV): characterization of four different isolates. Virology 76:569–580
    [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 277:680–684
    [Google Scholar]
  5. Morison W. L. 1975; Cell mediated immune responses in patients with warts. British Journal of Dermatology 93:553–556
    [Google Scholar]
  6. Orth G., Favre M., Croissant O. 1977; Characterization of a new type of human papilloma virus that causes skin warts. Journal of Virology 24:108–120
    [Google Scholar]
  7. Orth G., Dompmartin D., Agache P., Favre M., Croissant O. 1978; Pluralité du virus des papillomes humains. Annales de Dermatologie et de Vénéréologie (Paris) 105:279–288
    [Google Scholar]
  8. Parish C. R. 1971; Immune response to chemically modified flagellin. II. Evidence for a fundamental relationship between humoral and cell mediated immunity. Journal of Experimental Medicine 134:21–47
    [Google Scholar]
  9. Pfister H., Gissmann L., Zur Hausen H. 1977; Partial characterization of the proteins of human papilloma viruses (HPV) 1–3. Virology 83:131–137
    [Google Scholar]
  10. Reid T., Fraser N. G., Kernohan I. P. 1976; Generalized wart and immune deficiency. British Journal of Dermatology 95:559–564
    [Google Scholar]
  11. Staquet M. J., Viac J., Thivolet J., Chardonnet Y. 1978; Characterization of human papilloma virus (HPV) present in genital warts. Archives of Dermatology, Letters 261:77–79
    [Google Scholar]
  12. Thivolet J., Hegazy M. R., Viac J., Chardonnet Y. 1977; An in vivo study of cell-mediated immunity in human warts. Acta Dermato-Venereologica 57:317–321
    [Google Scholar]
  13. Thivolet J., Viac J. 1978; Immunologie des verrues humaines. Annales de Dermatologie et de Vénéréologie (Paris) 105:257–264
    [Google Scholar]
  14. Thompson K., Harris M., Benjamini E., Mitchell G., Noble M. 1972; Cellular and humoral immunity: a distinction in antigenic recognition. Nature New Biology 238:20–21
    [Google Scholar]
  15. Viac J., Thivolet J., Hegazy M. R., Chardonnet Y., Dambuyant C. 1977a; Comparative study of delayed hypersensitivity skin reactions and antibodies to human papilloma virus (HPV). Clinical and Experimental Immunology 29:240–246
    [Google Scholar]
  16. Viac J., Thivolet J., Chardonnet Y. 1977b; Specific immunity in patients suffering from recurring warts before and after repetitive intradermal tests with human papilloma virus. British Journal of Dermatology 97:365–369
    [Google Scholar]
  17. Viac J., Staquet M. J., Thivolet J. 1978; Relations antigeniques entre virus des papillomes humains (VPH). Etude expérimentale chez le cobaye. Annales d’Immunologie (Institut Pasteur) 129 C:559–570
    [Google Scholar]
  18. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-3-713
Loading
/content/journal/jgv/10.1099/0022-1317-43-3-713
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error