1887

Abstract

SUMMARY

Human fibroblast and human leucocyte interferons display a strong affinity for the copper chelate of bis-carboxymethyl amino agarose, binding tenaciously over a wide pH range (7.4 to 4.0). Their binding is apparently irreversible on a sorbent saturated with copper (24.8 µmol of Cu/ml of column bed). However, both interferons can be partially recovered from sorbents of lower copper content, prepared by leaching the columns with sodium citrate at pH 9.0. The recovery of fibroblast interferon from a leached sorbent (5.8 µmol of Cu/ml of column bed) is about 30% and that of leucocyte interferon about 60%. Moreover, the strength of binding of leucocyte interferon can be modulated by leaching copper chelate-agarose with citrate of varying concentration.

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1979-06-01
2021-10-25
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References

  1. Berthold W., Tan C., Tan Y. H. 1978; Purification and in vitro labeling of interferon from a human fibroblastoid cell line. Journal of Biological Chemistry 253:5206–5211
    [Google Scholar]
  2. Böhlen P., Stein S., Dairman W., Udenfriend S. F. 1973; Fluorimetric assay of proteins in the nanogram range. Archives of Biochemistry and Biophysics 155:213–220
    [Google Scholar]
  3. Bollin E. Jun, Sulkowski E. 1978; Metal chelate affinity chromatography of hamster interferon. Archives of Virology 58:149–152
    [Google Scholar]
  4. Chadha K. C., Sclair M., Sulkowski E., Carter W. A. 1978; Molecular size heterogeneity of human leukocyte interferon. Biochemistry 17:196–200
    [Google Scholar]
  5. Davey M. W., Sulkowski E., Carter W. A. 1976; Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction. Biochemistry 15:704–713
    [Google Scholar]
  6. Edy V. G., Billiau A., De somer P. 1977; Purification of human fibroblast interferon by zinc chelate affinity chromatography. Journal of Biological Chemistry 252:5934–5935
    [Google Scholar]
  7. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419–427
    [Google Scholar]
  8. Havell E. A., Vilcek J. 1972; Production of high-titred interferon in cultures of human diploid cells. Antimicrobial Agents and Chemotherapy 2:476–484
    [Google Scholar]
  9. Lebreton J. P. 1977; Purification of human plasma alpha-SH glycoprotein by zinc chelate affinity chromatography. FEBS Letters 80:351–354
    [Google Scholar]
  10. Lönnerdal B., Carlsson J., Porath J. 1977; Isolation of lactoferrin from human milk by metal-chelate affinity chromatography. FEBS letters 75:89–92
    [Google Scholar]
  11. Pidot A. L. R., O’keefe II G., McManus N., Mcintyre O. R. 1972; Human leukocyte interferon: the variation in normals and correlation with PHA transformation. Proceedings of the Society for Experimental Biology and Medicine 140:1263–1269
    [Google Scholar]
  12. Porath J., Carlsson J., Olsson I., Belfrage G. 1975; Metal chelate affinity chromatography, a new approach to protein fractionation. Nature, London 258:598–599
    [Google Scholar]
  13. Stewart II W. E., Desmyter J. 1975; Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation, and cross-species antiviral activity. Virology 67:68–73
    [Google Scholar]
  14. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus specific non-capsid proteins in poliovirus infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
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