Propagation and Characterization of a C-Type Virus from a Rhabdomyosarcoma of a Corn Snake Free

Abstract

SUMMARY

The presence of a C-type virus in tissues of an embryonic rhabdomyosarcoma of a corn snake was previously described, based upon electron microscopic observations. A virus, corn snake retrovirus (CSRV) has been recovered from the tumour tissue by inoculation of a tissue homogenate on to either the rattlesnake fibroma cell line or early passage cells derived from rattlesnake heart or kidney. Attempts to cultivate the virus in other reptilian cell systems were unsuccessful. The virus was classified as a retrovirus on the basis of electron microscopic observations of fine structure and morphogenesis, and the demonstration of virion-associated reverse transcriptase and a buoyant density of 1.16. Polypeptide analysis of CSRV performed by polyacrylamide gel electrophoresis revealed the presence of five major polypeptides: three had mobility analogous to that of structural polypeptides of viper retrovirus (VRV) but two polypeptides, one of mol. wt. approx. 16000 and a glycoprotein of mol. wt. approx. 72000, were unique. Antigenic comparison of CSRV and VRV by agar gel immunodiffusion revealed that CSRV possesses a major antigenic determinant which is different to that of VRV. CSRV propagated in rattlesnake fibroma cells was demonstrated to be slowly cytopathic for rattlesnake heart and kidney cells .

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1979-06-01
2024-03-29
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References

  1. Ahmed M., Korol W., Herles S., Yeh J., Mayasi S. 1974; Synctyia formation by Mason-Pfizer monkey virus (M-PMV) in a human cell culture carrying Rous sarcoma virus (RSV) genome. Bacteriological Proceedings 74:215
    [Google Scholar]
  2. Baltimore D., Smoler D. 1971; Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 68:1507–1511
    [Google Scholar]
  3. Bishop D. H. L., Ruprecht R., Simpson R. W., Spiegelman S. 1971; Deoxyribonucleic acid polymerase of Rous sarcoma virus: reaction conditions and analysis of the reaction product nucleic acids. Journal of Virology 8:730–741
    [Google Scholar]
  4. Clark H. F. 1971; Cultivation of cells from poikilothermic vertebrates. In Nutrition and Metabolism of Tissue Culture Cells vol II pp. 288–325 Edited by Rothblat G., Cristofalo V. J. New York: Academic Press;
    [Google Scholar]
  5. Clark H. F. 1973; Reptiles. In Tissue Culture Methods and Application pp. 147–150 New York and London: Academic Press;
    [Google Scholar]
  6. Clark H. F., Cohen M. M., Karzon D. T. 1970; Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 °C. Proceedings of the Society for Experimental Biology and Medicine 133:1039–1047
    [Google Scholar]
  7. Clark H. F., Cohen M. M., Lunger P. D. 1974; Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH-2) from Vipera russelli. Journal of the National Cancer Institute 51:645–657
    [Google Scholar]
  8. Clark H. F., Karzon D. T. 1976; Terrapene heart (TH-l), a continuous cell line from the heart of the box turtle Terapene Carolina. Experimental Cell Research 48:263–268
    [Google Scholar]
  9. Dion A. S., Vaidya A. B., Fout G. S. 1974; Cation preferences for poly(rC). oligo(dG)-directed DNA synthesis by RNA tumor viruses and human milk particulates. Cancer Research 34:3509–3515
    [Google Scholar]
  10. Fairbanks G., Steck T. L., Wallach D. F. H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
    [Google Scholar]
  11. Ferrer J. F., Diglio C. A. 1976; Development of an in vitro infectivity assay for the C-type bovine leukemia virus. Cancer Research 36:1068–1073
    [Google Scholar]
  12. Fryer J. L. 1964; Methods for the in vitro cultivation of cells from the tissues of salmonid fishes. Ph.D. thesis, Oregon State University. Library of Congress Card No. Microfilm 64–10, 510 Ann Arbor, Michigan: University Microfilms; pp. 198
    [Google Scholar]
  13. Gerard G. F. 1975; Poly(2′-O-methylcytidylate).oligodeoxyguanylate, a template specific for reverse transcriptase is not utilized by HeLa cell y DNA polymerase. Biochemical and Biophysical Research Communications 63:706–711
    [Google Scholar]
  14. Gerard G. F., Rottman F., Green M. 1974; Poly(2′-O-methylcytidylate).oligodeoxyguanylate as a template for the ribonucleic acid directed deoxyribonucleic acid polymerase in ribonucleic acid tumor virus particles and a specific probe for the ribonucleic acid directed enzyme in transformed murine cells. Biochemistry 13:1632–1641
    [Google Scholar]
  15. Goodman N. C., Spiegelman S. 1971; Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proceedings of the National Academy of Sciences of the United States of America 68:2203–2206
    [Google Scholar]
  16. Gravell M., Malsberger R. G. 1965; A permanent cell line from the fathead minnow (Pimephales promelas). Annals of the New York Academy of Sciences 126:555–565
    [Google Scholar]
  17. KlemenT V., Rowe W. P., Hartley J. W., Pugh W. E. 1969; Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proceedings of the National Academy of Sciences of the United States of America 63:753–758
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  19. Lunger P. D., Clark H. F. 1972; Simultaneous budding of ‘C-type’ particles and rhabdoviruses from individual reptilian cells. In vitro 7:337
    [Google Scholar]
  20. Lunger P. D., Hardy W. D. Jun, Clark H. F. 1974; C-type virus particles in a reptilian tumor. Journal of the National Cancer Institute 52:1231–1233
    [Google Scholar]
  21. Mcguire J. C., Pene J. J., Barrow-carraway J. 1974; Gene expression during the development of bacteriophage 29. III. Analysis of viral-specific protein synthesis with suppressible mutants. Journal of Virology 13:690–698
    [Google Scholar]
  22. Michalski F., Cohen M. M., Clark H. F. 1974; Adult and embryonic gecko cells in vitro growth characteristics, infection by rabies, sindbis and polyoma virus and transformation by SV40. Proceedings of the Society for Experimental Biology and Medicine 133:1039–1047
    [Google Scholar]
  23. Orr H. C., Harris L. E. Jun, Bader A. V., Kirschstein R. L., Probst P. G. 1972; Cultivation of cells from a fibroma in a rattlesnake, Crotalus horridus. Journal of the National Cancer Institute 48:259–264
    [Google Scholar]
  24. Rand K. H., Long C. W. 1973; Fusion of a Rous sarcoma virus transformed human cell line K.C., by RD–114 virus. Journal of General Virology 21:523–532
    [Google Scholar]
  25. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  26. Sokol F., Clark H. F., Wiktor T. J., Mcfalls M. L., Bishop D. H. L., Obijeski J. F. 1974; Structural phosphoproteins associated with ten rhabdoviruses. Journal of General Virology 24:433–445
    [Google Scholar]
  27. Temin H. M., Mizutani S. 1970; RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature, London 226:1211–1213
    [Google Scholar]
  28. Weissbach A., Bolden A., Muller R., Hanafusa H., Hanafusa T. 1972; Deoxyribonucleic acid polymerase activities in normal and leukosis-infected chicken embryo cells. Journal of Virology 10:321–327
    [Google Scholar]
  29. Wells R. D., Flugel R. M., Larson J. E., Schendel P. F., Sweet R. W. 1972; Comparison of some reactions catalysed by deoxyribonucleic acid polymerase from avian myeloblastosis virus, Escherichia coli and Micrococcus luteus. Biochemistry 11:621–629
    [Google Scholar]
  30. Wolf K., Gravell M., Malsberger R. G. 1966; Lymphocystis virus: isolation and propagation in centrarchid fish cell lines. Science 151:1004–1005
    [Google Scholar]
  31. Wolf K., Quimby M. C. 1962; Established eurythermic line of fish cells in vitro. Science 13s:1065–1066
    [Google Scholar]
  32. Wolf K., Quimby M. C. 1969; Fish cell and tissue culture. Fish Physiology 3:253–305
    [Google Scholar]
  33. Zeigel R. F., Clark H. F. 1971; Histologic and electron microscopic observations on a tumor-bearing viper: establishment of a ‘C’ type virus-producing cell line. Journal of the National Cancer Institute 46:309–321
    [Google Scholar]
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