1887

Abstract

SUMMARY

Interferons were stimulated in mouse L cells by Newcastle disease virus (NDV) or by polyriboinosinic-polyribocytidylic acid poly(rI). poly(rC). These were fractionated by sequential affinity chromatography on bovine plasma albumin (BPA)-Sepharose and on ω-carboxypentyl (CH)-Sepharose. Based on their interaction with CH-Sepharose, interferor induced by NDV was resolved into three major bands of activity (L/NDV-1,2,3) and poly(rI). Poly(rC)-interferon into two (L/rI:rC-1,2). These interferon components were purified to a specific activity of 3 × 10 to 4 × 10 units/mg protein by antibody affinity chromatography and examined by electrophoresis in SDS-polyacrylamide gels. A total of five molecular species was thus identified for NDV-induced interferon and three for poly(rI). poly(rC) induced interferon, as summarized in Table 1. We conclude from our observations that mouse interferons can be produced by L cells in multiple forms with specific physiochemical properties and in proportions determined by the type of agent employed for induction.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-3-521
1979-06-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/3/JV0430030521.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-3-521&mimeType=html&fmt=ahah

References

  1. Borecký L., Fuchsberger N., Hájnická V., Stanček D., Zemla J. 1972; Distribution of antiviral and cell-inhibitory activity in interferon preparations. Acta Virologica 16:356–358
    [Google Scholar]
  2. Boxaca M., Paucker K. 1967; Neutralization of different murine interferons by antibody. Journal of Immunology 98:1130–1135
    [Google Scholar]
  3. Cesario T., Schryer P., Tilles J. 1977; Relationship between the physicochemical nature of human interferon, the cell induced, and the inducing agent. Antimicrobial Agents and Chemotherapy 11:291–298
    [Google Scholar]
  4. Davey M. W., Sulkowski E., Carter W. A. 1976; Purification and characterization of mouse interferon with novel affinity sorbents. Journal of Virology 17:439–445
    [Google Scholar]
  5. De Maeyer-Guignard J., Tovey M. G., Gresser I., De Maeyer E. 1978; Purification of mouse interferon by sequential affinity chromatography on poly(U)- and antibody-agarose columns. Nature, London 271:622–625
    [Google Scholar]
  6. Fauconnier B. 1967a; Parenté antigénique des interférons induits par des agents viraux ou bactériens et par le statolon chez la Souris. Comptes Rendus hebdomadaires des seances de l’Académie des Sciences, Paris 264:1814–1817
    [Google Scholar]
  7. Fauconnier B. 1976b; Antigenic identity of interferons induced by different viruses in the same cell system. Nature, London 214:591–592
    [Google Scholar]
  8. Golgher R. R., Paucker K. 1973; Blocking of interferon production by chromatographically purified L cell interferon. Proceedings of the Society for Experimental Biology and Medicine 142:167–174
    [Google Scholar]
  9. Havell E. A., Hayes T. G., Vilcek J. 1978; Synthesis of two distinct interferons by human fibroblasts. Virology 89:330–334
    [Google Scholar]
  10. Jankowski W. J., Davey M. W., O’Malley J. A., Sulkowski E., Carter W. A. 1975; Molecular structure of human fibroblast and leukocyte interferons; probe by lectin and hydrophobic chromatography. Journal of Virology 16:1124–1130
    [Google Scholar]
  11. Kawade Y. 1973; Purificxation and characterization of mouse L cell interferon. Japanese Journal of Microbiology 17:129–140
    [Google Scholar]
  12. Kawakita M., Cabrer B., Taira H., Rebello M., Slattery E., WelDell H., Lengyel P. 1978; Purification of interferon from mouse Ehrlich ascites tumor cells. Journal of Biological Chemistry 253:598–602
    [Google Scholar]
  13. Knight E. Jun 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  14. Lin L. S., Wiranowska-Stewart M., Chudzio T., Stewart II W. E. 1978; Characterization of the size and charge heterogeneities of human leukocyte interferon populations. Archives of Virology 56:269–272
    [Google Scholar]
  15. Maehara N., Ho M., Armstrong J. A. 1977; Differences in mouse interferons according to cell source and mode of induction. Infection and Immunity 17:572–579
    [Google Scholar]
  16. Ogburn C. A., Berg K., Paucker K. 1973; Purification of mouse interferon by affinity chromatography on anti-interferon globulin-Sepharose. Journal of Immunology 111:1206–1218
    [Google Scholar]
  17. Paucker K., Stanček D. 1972; Characterization of interferon-associated proteins. Journal of General Virology 15:129–138
    [Google Scholar]
  18. Paucker K., Berman B. J., Golgher R. R., Stanček D. 1970; Purification, characterization and attempts at isotopic labeling of mouse interferon. Journal of Virology 5:145–152
    [Google Scholar]
  19. Paucker K., Dalton B. J., Ogburn C. A., Torma E. 1975; Multiple active sites on human interferons. Proceedings of the National Academy of Sciences of the United States of America 72:4587–4591
    [Google Scholar]
  20. Salvin S. B., Youngner J. S., Nishio J., Neta R. 1975; Tumor suppression by a lymphokine released into the circulation of mice with delayed hypersensitivity. Journal of the National Cancer Institute 55:1233–1236
    [Google Scholar]
  21. Stanček D., Paucker K. 1971; Preparative electrophoresis of isotopically labeled L-cell interferons. Applied Microbiology 21:1067–1071
    [Google Scholar]
  22. Stanček D., Gressnerova M., Paucker K. 1970; Isoelectric components of mouse, human and rabbit interferons. Virology 41:740–750
    [Google Scholar]
  23. Stewart II W. E. 1974; Distinct molecular species of interferons. Virology 61:80–86
    [Google Scholar]
  24. Stewart II W. E., Desomer P., Declercq E. 1974; Protective effects of anionic detergents on interferons: reversible denaturation. Biochimica et Biophysica Acta 359:364–368
    [Google Scholar]
  25. Stewart II W. E., Gresser I., Tovey M. G., Bandu M.-T., Legoff S. 1976; Identification of the cell multiplication inhibitory factors in interferon preparations as interferons. Nature, London 262:300–302
    [Google Scholar]
  26. Stewart II W. E., Legoff S., Wiranowska-Stewart M. 1977; Characterization of two distinct molecular populations of type 1 mouse interferons. Journal of General Virology 37:277–284
    [Google Scholar]
  27. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by Dodecyl Sulfate-Polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  28. Weiner A. M., Platt T., Weber K. 1972; Amino-terminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis. Journal of Biological Chemistry 247:3242–3251
    [Google Scholar]
  29. Yamamoto Y., Kawade Y. 1976; Purification of two components of mouse L cell interferon: electrophoretic demonstration of interferon proteins. Journal of General Virology 33:225–236
    [Google Scholar]
  30. Yamamoto Y., Tsukui K., Ohwaki M., Kawade Y. 1974; Electrophoretic characterization of purified mouse L cell interferon of high specific activity. Journal of General Virology 23:23–32
    [Google Scholar]
  31. Youngner J. S., Salvin S. B. 1973; Production and properties of migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. Journal of Immunology 111:1914–1922
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-3-521
Loading
/content/journal/jgv/10.1099/0022-1317-43-3-521
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error