1887

Abstract

SUMMARY

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SA11 is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SA11 produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-3-513
1979-06-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/3/JV0430030513.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-3-513&mimeType=html&fmt=ahah

References

  1. Almeida J. D., Hall T., Banatvala J. E., Totterdell B. M., Chrystie I. L. 1978; The effect of trypsin on the growth of rotavirus. Journal of General Virology 40:213–218
    [Google Scholar]
  2. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. 1977; Rotavirus isolation and cultivation in the presence of trypsin. Journal of Clinical Microbiology 6:610–617
    [Google Scholar]
  3. Estes M. K., Graham D. Y., Smith E. M., Gerba C. P. 1979; Rotavirus stability and inactivation. Journal of General Virology 43: (in the press)
    [Google Scholar]
  4. Graham D. Y. 1977; Enzyme replacement therapy of exocrine pancreatic insufficiency in man: relation between in vitro enzyme activities and in vivo potency in commercial pancreatic extracts. New England Journal of Medicine 396:1314–1317
    [Google Scholar]
  5. Kaplan M. M., Wiktor T. J., Maes R. F., Campbell J. B., Koprowski H. 1967; Effect of polyions on the infectivity of rabies virus in tissue culture: construction of a single-cycle growth curve. Journal of Virology 1:145–151
    [Google Scholar]
  6. Mcnulty M. S. 1978; Rotaviruses. Journal of General Virology 40:1–18
    [Google Scholar]
  7. Malherbe H. H., Strickland-Cholmley M. 1967; Simian virus SA11 and the related O agent. Archiv für die gesamte Virusforschung 22:235–245
    [Google Scholar]
  8. Matsuno S., Inouye S., Kono R. 1977; Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. Journal of Clinical Microbiology 5:1–4
    [Google Scholar]
  9. Schoub B. D., Lecatsas G., Prozesky O. W. 1977; Antigenic relationship between human and simian rotaviruses. Journal of Medical Microbiology 10:1–6
    [Google Scholar]
  10. Spendlove R. S., Schaffer F. L. 1965; Enzymatic enhancement of infectivity of reovirus. Journal of Bacteriology 89:597–602
    [Google Scholar]
  11. Takemoto K. K. 1966; Plaque mutants of animal viruses. In Progress in Medical Virology vol 8 pp. 314–348 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  12. Wallis C., Melnick J. L. 1968; Mechanism of enhancement of virus plaques by cationic polymers. Journal of Virology 2:267–274
    [Google Scholar]
  13. Wallis C., Melnick J. L., Rapp F. 1966; Effects of pancreatin on the growth of reovirus. Journal of Bacteriology 92:155–160
    [Google Scholar]
  14. Wyatt R. G., Kalica A. R., Mebus C. A., Kim H. W., London W. T., Chanock R. M., Kapikian A. Z. 1978; Reovirus-like agents (rotaviruses) associated with diarrheal illness in animals and man. In Perspectives in Virology vol 10 pp 121–145 Edited by Pollard M. New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-3-513
Loading
/content/journal/jgv/10.1099/0022-1317-43-3-513
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error