1887

Abstract

SUMMARY

An enveloped, ether-sensitive, acid-labile virus isolated from , the bluegill, is described. Virus replication is limited to teleostean cell lines and achieves highest titres in centrarchid cells. As determined by the uptake of tritiated nucleotides and the effect of 6-azauridine and actinomycin D on replication, the virus genome is RNA. The virus has no haemagglutinin or neuraminidase nor does it share antigens with prototypes of orthomyxoviruses, paramyxoviruses or arenaviruses. Thin-section electron microscopy reveals a virion 80 to 100 nm in diam. associated with the cell surface and intracellular vacuoles. The virus appears to mature by budding and exhibits a prolonged replication cycle and significant cell-associated infectivity. The time sequence of replication indicates that the prolonged cycle is due to maturation rather than to delayed synthesis of RNA and protein. The bluegill virus (BGV) represents a novel, hitherto undescribed, type of teleostean virus.

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/content/journal/jgv/10.1099/0022-1317-43-3-489
1979-06-01
2024-12-06
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References

  1. Beasley A., Sigel M. M., Clem L. 1965; Latent infection in marine fish cell cultures. Proceedings of the Society for Experimental Biology and Medicine 121:1169–1174
    [Google Scholar]
  2. Bonami J. R., Veyrunes J. C., Cousserans F., Vago C. 1975; Ultrastructure, développement et acide nucléique du virus S du crustace decapode Macropipus depurator. Comptes Rendus hebdomadaires des seances de l’ Academic des Sciences series D 280:359–361
    [Google Scholar]
  3. Federici B. A., Hazard E. I. 1975; Iridovirus and polyhedrosis virus in the freshwater daphnid Simocephalus expinosus. Nature, London 254:327–328
    [Google Scholar]
  4. Hoffmann G. L., Dunbar C. E., Wolf K., Zwillenberg L. O. 1969; Epitheliocystis, a new infectious disease of the bluegill. Antonie van Leeuwenhoek 35:146–158
    [Google Scholar]
  5. Holmes I. H. 1971; Morphological similarity of Bunyamwera super group viruses. Virology 43:708–712
    [Google Scholar]
  6. Laver W. G., Kilbourne E. D. 1966; Identification in a recombinant influenza virus of structural proteins derived from both parents. Virology 30:493–501
    [Google Scholar]
  7. Lennette E. H., Schmidt N. J. 1964; Diagnostic procedures for viral and ricketssial diseases. American Public Health Association, New York pp 51:
    [Google Scholar]
  8. Mannweiler K., Lehmann-Grube F. 1973; Electron microscopy of LCM virus-infected L cells. In Lymphocytic Choriomeningitis Virus and other Arenaviruses pp. 37–48 Edited by Lehman-Gnrube F. Berlin: Springer Verlag;
    [Google Scholar]
  9. Milne R. G. 1967; Plant viruses inside cells. Science Progress, Oxford 55:203–222
    [Google Scholar]
  10. Murphy F. A., Webb P. A., Johnson K. M., Whitefield S. G., Chappell M. A. 1970; Arenaviruses in Vero cells: ultrastructural studies. Journal of Virology 6:507–518
    [Google Scholar]
  11. Nicholson B., Byrne C. 1973; An established cell line from the Atlantic salmon (Salmo salar). Journal of the Fish Research Board of Canada 30:913–916
    [Google Scholar]
  12. Obijeski J. F., Murphy F. A. 1977; Bunyaviridae: recent biochemical developments. Journal of General Virology 37:1–14
    [Google Scholar]
  13. Oshiro L. S., Schieble J. H., Lennette E. H. 1971; Electron microscopic studies of coronaviruses. Journal of General Virology 12:161–168
    [Google Scholar]
  14. Pons M. 1967; Effect of actinomycin D on the replication of influenza virus and influenza virus RNA. Virology 33:150–154
    [Google Scholar]
  15. Porterfield J. S., Casals J., Chumankov M. P., Gaidamovich S. Ya., Hannoun C., Holmes I. H., Horzinek M. C., Mussoay M., Oker-Blom N., Russell P. K. 1976; Bunyaviruses and Bunyaviridae. Intervirology 6:13–24
    [Google Scholar]
  16. Portner A., Bussell R. 1973; Measles virus ribonucleic acid and protein synthesis. Effects of 6-azauridine and cycloheximide on viral replication. Journal of Virology 11:46–53
    [Google Scholar]
  17. Rott R., Scholtissek C. 1964; Einfluss von Actinomicin auf die Vermerung von Myxoviren. Zeitschrift für Naturforschung 196:316–323
    [Google Scholar]
  18. Rowe W. P., Murphy F. A., Bergold G. H., Casals J., Hotchkin J., Johnson K. M., Lehmann-Grube F., Mims C. A., Traub E., Webb P. A. 1970; Arenaviruses: proposed name for a newly defined virus group. Journal of Virology 5:651–652
    [Google Scholar]
  19. Wolf K., Darlington R. W. 1971; Channel catfish virus, a new herpesvirus of ictalurid fish. Journal of Virology 8:525–533
    [Google Scholar]
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