1887

Abstract

SUMMARY

The virus-specific nucleotide sequences in the RNA and DNA of a Kirsten mouse sarcoma virus (Ki-MSV)-transformed non-producer human osteosarcoma cell clone and two subclones of these cells that reverted to a normal phenotype have been analysed by hybridization of sarcoma virus-specific complementary DNA (cDNA) to cellular RNA or DNA. Whereas the transformed clone had acquired Ki-MSV sequences in the RNA and DNA of the cells, both the revertant cell lines seemed to have lost most or all of this information from the cellular nucleic acids. The DNA from the revertant cells lacked the sequences represented either in the Ki-MSV-specific cDNA or in the total cDNA of the leukaemia-sarcoma virus complex. Thus, the reversion of the virus-transformed human cells to normal morphology is associated with the loss of most or all of the proviral sequences from the cellular DNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-2-447
1979-05-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/2/JV0430020447.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-2-447&mimeType=html&fmt=ahah

References

  1. Bensinger W. I., Robbins K. C., Greenberger J. S., Aaronson S. A. 1977; Different mechanisms for morphologic reversion of a clonal population of murine sarcoma virus-transformed nonproducer cells. Virology 77:750–761
    [Google Scholar]
  2. Britten R. J., Smith J. 1970; A bovine genome. Carnegie Institute Yearbook 68:378–386
    [Google Scholar]
  3. Cho H. Y., Cutchins E. C., Rhim J. S., Huebner R. J. 1976; Revertants of human cells transformed by murine sarcoma virus. Science 194:951–953
    [Google Scholar]
  4. Deng C.-T., Boettiger D., Macpherson I., Varmus H. E. 1974; The persistence and expression of virus-specific DNA in revertants of Rous sarcoma virus-transformed BHK-21 cells. Virology 62:512–521
    [Google Scholar]
  5. Deng C.-T., Stehelin D., Bishop J. M., Varmus H. E. 1977; Characteristics of virus-specific RNA in avian sarcoma virus-transformed BHK-21 cells and revertants. Virology 76:313–330
    [Google Scholar]
  6. Dulbecco R. 1976; From the molecular biology of oncogenic RNA viruses to cancer. Science 192:437–440
    [Google Scholar]
  7. Frankel A. E., Haapala D. K., Neubauer R. L., Fischinger P. J. 1976; Elimination of the sarcoma genome from murine sarcoma virus transformed cat cells. Science 191:1264–1266
    [Google Scholar]
  8. Gazdar A. F., Stull H. B., Chopra H. C., Ikawa Y. 1974; Properties of flat variants of murine sarcoma virus transformed non-producer cells isolated after high-temperature passage. International Journal of Cancer 13:219–226
    [Google Scholar]
  9. Krzyzek R. A., Lau A. F., Faras A. J., Spector D. H. 1977; Post-transcriptional control of avian oncornavirus transforming gene sequences in mammalian cells. Nature, London 269:175–179
    [Google Scholar]
  10. Niman H. L., Gardner M. B., Stephenson J. R., Roy-Burman P. 1977a; Endogenous RD-114 virus genome expression in malignant tissues of domestic cats. Journal of Virology 23:578–586
    [Google Scholar]
  11. Niman H. L., Stephenson J. R., Gardner M. B., Roy-Burman P. 1977b; RD-114 and feline leukaemia virus genome expression in natural lymphomas of domestic cats. Nature, London 226:357–360
    [Google Scholar]
  12. Nomura S., Fischinger P. J., Mattern C. F. T., Peebles P. T., Bassin R. H., Friedman G. P. 1972; Revertants of mouse cells transformed by murine sarcoma virus. I. Characterization of flat and transformed sublines without a rescuable murine sarcoma virus. Virology 50:51–64
    [Google Scholar]
  13. Pollack R. E., Green H., Todaro G. J. 1968; Growth control in cultured cells: selection of sublines with increased sensitivity to contact inhibition and decreased tumor-producing ability. Proceedings of the National Academy of Sciences of the United States of America 60:126–133
    [Google Scholar]
  14. Pollack R. E., Wolman S., Vogel A. 1970; Reversion of virus-transformed cell lines: hyperploidy accompanies retention of viral genes. Nature, London 228:938–970
    [Google Scholar]
  15. Rabinowitz Z., Sachs L. 1968; Reversion of properties in cells transformed by polyoma virus. Nature, London 220:1203–1206
    [Google Scholar]
  16. Rabinowitz Z., Sachs L. 1970; Control of the reversion of properties in transformed ceils. Nature, London 225:136–139
    [Google Scholar]
  17. Rasheed S., Bruszewski J., Rongey R. W., Roy-Burman P., Charman H. P., Gardner M. B. 1976; Spontaneous release of endogenous ecotropic type C virus from rat embryo cultures. Journal of Virology 18:799–803
    [Google Scholar]
  18. Rhim J. S., Cho H. Y., Huebner R. J. 1975; Non-producer human cells induced by murine sarcoma virus. International Journal of Cancer 15:23–29
    [Google Scholar]
  19. Roy-Burman P., Klement V. 1975; Derivation of mouse sarcoma virus (Kirsten) by acquisition of genes from heterologous host. Jouroal of General Virology 28:193–198
    [Google Scholar]
  20. Scolnick E. M., Rands E., Williams D., Parks W. P. 1973; Studies on the nucleic acid sequences of Kirsten sarcoma virus: a model for formation of a mammalian RNA-containing sarcoma virus. Journal of Virology 12:458–463
    [Google Scholar]
  21. Shih T. Y., Young H. A., Coffin J. M., Scolnick E. M. 1978; Physical map of the Kirsten sarcoma virus genome as determined by fingerprinting RNase T1-resistant oligonucleotides. Journal of Virology 25:238–252
    [Google Scholar]
  22. Stehelin D., Guntaka R. V., Varmus H. E., Bishop J. M. 1976; Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses. Journal of Molecular Biology 101:349–365
    [Google Scholar]
  23. Stephenson J. R., Scolnick E. M., Aaronson S. A. 1972; Genetic stability of the sarcoma viruses in murine and avian sarcoma virus-transformed nonproducer cells. International Journal of Cancer 9:577–583
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-2-447
Loading
/content/journal/jgv/10.1099/0022-1317-43-2-447
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error