1887

Abstract

SUMMARY

An assay for virus antibodies using protein A from is described. Type B and type C RNA tumour viruses adsorbed on to polystryrene microtitre plate wells were incubated with antiserum and then with I-labelled protein A (I-pA) and bound radioactivity was determined. Technical details such as labelling, antigen concentration, storage of I-pA are reported. The specificity of the reaction was investigated in detail by competition experiments with purified unbound homologous viruses. This assay also proved to be sensitive for demonstration of autogenous immunity to both type B and type C RNA tumour viruses. A study using antisera against purified core and envelope virus proteins of mammary tumour and leukaemia viruses suggested that the reaction mainly involves surface antigens of the intact virions.

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1979-05-01
2021-10-20
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References

  1. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. 1974; A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology 60:595–601
    [Google Scholar]
  2. Brown J. P., Klizman J. M., Hellstrӧm K. E. 1977; A micro-assay for antibody binding to tumor cell surface antigens using 125I-labelled protein A from Staphylococcus aureus. Journal of Immunological Methods 15:57–66
    [Google Scholar]
  3. Colombarrr A., De Rossi A., Hilkens J., Collavo D., Chieco-Bianchi L. 1979; Age dependence and genetics of expression of ecotropic MuLV in a SJL/J substrain. Journal of the National Cancer Institute (in the press)
    [Google Scholar]
  4. Dalrymple J. M., Teramoto A. Y., Cardiff R. D., Russell P. K. 1972; Radioimmune precipitation of group A arborviruses. Journal of Immunology 109:426–433
    [Google Scholar]
  5. Dorval G., Welsh K. I., Wigzell H. 1975; A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein A from Staphylococcus aureus. Journal of Immunological Methods 7:237–250
    [Google Scholar]
  6. Forsgen A., Sjӧquist J. 1966; Protein A from S. aureus, 1. Pseudo-immune reaction with human γ-globulin. Journal of Immunology 97:822–827
    [Google Scholar]
  7. Gerloff R. K., Hoyer B. H., McClaren L. C. 1962; Precipitation of radiolabelled poliovirus with specific antibody and antiglobulins. Journal of Immunology 89:559–566
    [Google Scholar]
  8. Hunter W. M. 1967; The preparation of radioiodinated proteins of high activity, their reaction with antibody in vitro: the radioimmunoassay. In Handbook of Experimental Immunology pp 608–642 Edited by Weir E. M. Philadelphia: F. A. Davies Co;
    [Google Scholar]
  9. Ihle J. N., Yurconic M. Jun, Hanna M. G. Jun 1973; Autogenous immunity to endogenous RNA tumour virus. Radioimmune precipitation assay of mouse serum antibody levels. Journal of Experimental Medicine 138:194–208
    [Google Scholar]
  10. Ihle J. N., Arthur L. O., Fine D. L. 1976; Autogenous immunity to mouse mammary tumor virus in mouse strains of high and low mammary tumor incidence. Cancer Research 36:2840–2844
    [Google Scholar]
  11. Kronvall G., Grey H. M., Williams R. C. Jun 1970a; Protein A reactivity with mouse immunoglobulins. Structural relationship between some mouse and human immunoglobulins. Journal of Immunology 105:1116–1123
    [Google Scholar]
  12. Kronvall G., Seal U. S., Finstad J., Williams R. C. Jun 1970b; Phylogenetic insight into evolution of mammalian Fc fragment of γ G globulin using Staphylococcus protein A. Journal of Immunology 104:140–147
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Mackenzie M. R., Warner N. L., Mitchell G. F. 1978; The binding of murine immunoglobulins to Staphylococcus protein A. Journal of Immunology 120:1493–1496
    [Google Scholar]
  15. Nowinski R. C., Kaehler S. L. 1974; Antibody to leukemia virus: widespread occurrence in inbred mice. Science 185:869–871
    [Google Scholar]
  16. Parks W. P., Howk R. S., Scolnick E. M., Oroszlan S., Gilden R. V. 1974; Immunochemical characterization of two major polypeptides from murine mammary tumor virus. Journal of Virology 13:1200–1210
    [Google Scholar]
  17. Strand M., August J. T. 1974; Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. Journal of Virology 13:171–180
    [Google Scholar]
  18. Wiktor T. J., Koprowski H., Dixon F. 1972; Radioimmunoassay procedure for rabies binding antibodies. Journal of Immunology 109:464–470
    [Google Scholar]
  19. Zelter P. M., Seeger R. C. 1977; Microassay using radioiodinated protein A from Staphylococcus aureus for antibodies bound to cell surface antigens of adherent tumor cells. Journal of Immunological Methods 17:163–175
    [Google Scholar]
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