RT Journal Article SR Electronic(1) A1 Gregerson, Dale S. A1 Russell, Paul A1 Reid, Ted W.YR 1979 T1 Biochemical and Immunological Properties of the Reverse Transcriptase Associated with a Hamster Retrovirus JF Journal of General Virology, VO 43 IS 2 SP 327 OP 337 DO https://doi.org/10.1099/0022-1317-43-2-327 PB Microbiology Society, SN 1465-2099, AB SUMMARY Several properties of an RNA-directed DNA polymerase associated with a hamster retrovirus (HaRV) were examined and found to be similar to other polymerases from mammalian type-C viruses in that the enzyme (i) is more active with Mn2+ than Mg2+, (ii) uses the reverse transcriptase-specific poly(rCm).oligo(dG) template, (iii) possesses substantial endogenous polymerase activity and (iv) is strongly inhibited by homologous antisera and moderately inhibited by antisera directed against other type-C viruses. In contrast to previous reports of polymerases from other hamster viruses, HaRV polymerase is active in endogenous assays and the activity is associated with a 70000 mol. wt. polypeptide in highly purified virions and with 70000 and 85000 mol. wt. polypeptides in fresh, unpurified virus. Only one major peak of polymerase activity eluted from DEAE-cellulose while subsequent elution of this peak from phosphocellulose produced two major peaks of polymerase activity. The mol. wt. of these two peaks were 70000 and 85000 by glycerol density-gradient sedimentation. The HaRV reverse transcriptase and p30 were found to be most closely related antigenically to other rodent retrovirus proteins., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-43-2-327