1887

Journal of General Virology header logo

Volume 43, Issue 2

Biochemical and Immunological Properties of the Reverse Transcriptase Associated with a Hamster Retrovirus Free

Abstract

SUMMARY

Several properties of an RNA-directed DNA polymerase associated with a hamster retrovirus (HaRV) were examined and found to be similar to other polymerases from mammalian type-C viruses in that the enzyme (i) is more active with Mn than Mg, (ii) uses the reverse transcriptase-specific poly(rCm).oligo(dG) template, (iii) possesses substantial endogenous polymerase activity and (iv) is strongly inhibited by homologous antisera and moderately inhibited by antisera directed against other type-C viruses. In contrast to previous reports of polymerases from other hamster viruses, HaRV polymerase is active in endogenous assays and the activity is associated with a 70000 mol. wt. polypeptide in highly purified virions and with 70000 and 85000 mol. wt. polypeptides in fresh, unpurified virus. Only one major peak of polymerase activity eluted from DEAE-cellulose while subsequent elution of this peak from phosphocellulose produced two major peaks of polymerase activity. The mol. wt. of these two peaks were 70000 and 85000 by glycerol density-gradient sedimentation. The HaRV reverse transcriptase and p30 were found to be most closely related antigenically to other rodent retrovirus proteins.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1979
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-2-327
1979-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/2/JV0430020327.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-2-327&mimeType=html&fmt=ahah

References

  1. Aaronson S. A., Parks W. P., Scolnick E. M., Todaro G. J. 1971; Antibody to the RNA-dependent DNA polymerase of mammalian C-type RNA tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 68:920–924
     [Google Scholar]
  2. Baltimore D. 1970; RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature, London 226:1209–1211
     [Google Scholar]
  3. Bandyopadhyay A. K. 1977; Effect of Rauscher leukemia virus-specific proteins on reverse transcriptase. Journal of Biological Chemistry 252:5883–5887
     [Google Scholar]
  4. Bradford M. H. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
     [Google Scholar]
  5. Charman H. P., White M. H., Rahman R., Gilden R. V. 1976; Species and interspecies radioimmunoassays for rat type-C virus p 30: interviral comparisons and assay of human tumor extracts. Journal of Virology 17:51–59
     [Google Scholar]
  6. Gerard G. F., Grandgennett D. P. 1975; Purification and characterization of the DNA polymerase and RNase H activities in Moloney murine sarcoma-leukemia virus. Journal of Virology 15:785–797
     [Google Scholar]
  7. Gerard G. F., Rottman F., Green M. 1974; Poly(2′-0-methyl-cytidilate).oligodeoxyguanylate as a template for the ribonucleic acid directed deoxyribonucleic acid polymerase in ribonucleic acid tumor virus particles and a specific probe for the ribonucleic acid directed enzyme in transformed murine cells. Biochemistry 13:1632–1641
     [Google Scholar]
  8. Gerwin B. I., Smith S. G., Peebles P. T. 1975; Two active forms of RD-114 virus DNA polymerase in infected cells. Cell 6:45–52
     [Google Scholar]
  9. Malamy M. H., Horecker B. 1964; Purification and crystallization of the alkaline phosphatase of Escherichia coli. Biochemistry 3:1893–1897
     [Google Scholar]
  10. Moelling K. 1976; Further characterization of the Friend murine leukemia virus reverse transcriptase-RNase H complex. Journal of Virology 18:418–425
     [Google Scholar]
  11. Nakajima K., Ono K., Ito Y. 1974; Interconversion of molecular size of the DNA polymerase from Rauscher leukemia virus. Intervirology 3:332–340
     [Google Scholar]
  12. Okabe H., Gilden R. V., Hatanaka M. 1974; Specificity of the DNA product of RNA-dependent DNA polymerase in type-C viruses. Ⅲ. Analysis of viruses derived from Syrian hamsters. Proceedings of the National Academy of Sciences of the United States of America 71:3278–3282
     [Google Scholar]
  13. Parks W. P., Scolnick E. M., Ross J., Todaro G. J., Aaronson S. S. 1972; Immunological relationships of reverse transcriptases from ribonucleic acid tumor viruses. Journal of Virology 9:110–115
     [Google Scholar]
  14. Russell P., Gregerson D. S., Albert D. M., Reid T. W. 1979; Characteristics of a retrovirus associated with a hamster melanoma. Journal of General Virology (previous paper)
    [Google Scholar]
  15. Sherr C. J., Fedele L. A., Benveniste R. E., Todaro G. J. 1975; Interspecies antigenic determinants of the reverse transcriptases and P30 proteins of mammalian type C viruses. Journal of Virology 15:1440–1448
     [Google Scholar]
  16. Somers K. D., May J. T., Kit S., McCormick K. J., Hatch G. G., Stenback W. A., Trentin J. J. 1973; Biochemical properties of a defective hamster C-type oncornavirus. Intervirology 1:11–18
     [Google Scholar]
  17. Strand M., August J. T. 1975; Structural proteins of mamalian RNA tumor viruses; relatedness of the interspecies antigenic determinants of the major internal protein. Journal of Virology 15:1332–1341
     [Google Scholar]
  18. Temin H. M., Mizutani S. 1970; RNA-dependent DNA polymerase in virions of Rous sacroma virus. Nature, London 226:1211–1213
     [Google Scholar]
  19. Verma I. M. 1977; The reverse transcriptase. Biochimica et Biophysica Acta 473:1–38
     [Google Scholar]
  20. Verma I. M., Meuth N. L., Fan H., Baltimore D. 1974; Hamster leukemia virus: lack of endogenous DNA synthesis and unique structure of its DNA polymerase. Journal of Virology 13:1075–1082
     [Google Scholar]
  21. Weimann B. J., Schmidt J., Wolfrum D. I. 1974; RNA-dependent DNA polymerase and ribonuclease H from Friend virions. FEBS Letters 43:37–44
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-2-327
Loading
Biochemical and Immunological Properties of the Reverse Transcriptase Associated with a Hamster Retrovirus
J. Gen. Virol. 43, 327 (1979); https://doi.org/10.1099/0022-1317-43-2-327
/content/journal/jgv/10.1099/0022-1317-43-2-327
/content/journal/jgv/10.1099/0022-1317-43-2-327
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error