1887

Abstract

SUMMARY

Polyacrylamide gel electrophoresis of bovine rotavirus or neonatal calf diarrhoea virus (NCDV) grown in cell culture resolved eight species of polypeptide. The inner shell particles contained five polypeptides and the outer shell three polypeptides. A major polypeptide of the outer shell was glycosylated. The infectivity of NCDV was enhanced by treatment with trypsin . All eight polypeptides were affected by trypsin treatment as judged by diminished intensity of polypeptide bands by radiography and several new bands appeared. The intracellular synthesis of NCDV polypeptides was studied by pulse and pulse-chase experiments. Infected cells contained all eight virus capsid proteins and, in addition, three presumably virus-specific polypeptides which were non-capsid polypeptides (NCVP). There was no evidence that any of these polypeptides was processed after synthesis. It is suggested, therefore, that all these polypeptides are primary gene products.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-2-309
1979-05-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/2/JV0430020309.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-2-309&mimeType=html&fmt=ahah

References

  1. Almeida J. D., Hall T., Banatvala J. E., Totterdell B. M., Chrystie I. L. 1978; The effect of trypsin on the growth of rotavirus. Journal of General Virology 40:213–218
    [Google Scholar]
  2. Babiuk L. A., Mohammed K., Spence L., Fauvel M., Petro R. 1977; Rotavirus isolation and cultivation in the presence of trypsin. Journal of Clinical Microbiology 6:610–617
    [Google Scholar]
  3. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. 1973; Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet ii:1281–1283
    [Google Scholar]
  4. Bridger J. C., Woode G. M. 1976; Characterization of two particle types of calf rotavirus. Journal of General Virology 31:245–250
    [Google Scholar]
  5. Bryden A. S., Thouless M. E., Flewett T. H. 1976; A rabbit rotavirus. Veterinary Record 99:323
    [Google Scholar]
  6. Davidson G. P., Bishop R. F., Townley R. R. W., Holmes I. H., Ruck B. J. 1975; Importance of a new virus in acute sporadic enteritis in children. Lancet i:242–246
    [Google Scholar]
  7. Flewett T. H., Bryden A. S., Davies H. 1973; Virus particles in gastroenteritis. Lancet ii:1497
    [Google Scholar]
  8. Flewett T. H., Bryden A. S., Davies H. 1975; Virus diarrhoea in foals and other animals. Veterinary Record 96:477
    [Google Scholar]
  9. Holmes I. H., Ruck B. J., Bishop R. F., Davidson G. P. 1975; Infantile enteritis viruses: morphogenesis and morphology. Journal of Virology 16:937–943
    [Google Scholar]
  10. Kalica A. R., Garon C. F., Wyatt R. G., Mebus C. A., Vankirk D. H., Chanock R. M., Kapikian A. Z. 1976; Differentiation of human and calf reovirus-like agents associated with diarrhoea using polyacrylamide gel electrophoresis of RNA. Virology 74:86–92
    [Google Scholar]
  11. Kraft L. M. 1957; Studies on the etiology and transmission of epidemic diarrhoea of infant mice. Journal of Experimental Medicine 101:743–755
    [Google Scholar]
  12. Lamb R. A., Choppin P. W. 1977; The synthesis of Sendai virus polypeptides in infected cells. III. Phosphorylation of polypeptides. Virology 81:382–397
    [Google Scholar]
  13. McNulty M. S., Allan G. M., Pearson G. R., McFerran J. B., Curran W. L., McCracken R. M. 1976; Reovirus-like agent (rotavirus) from lambs. Infection and Immunity 14:1332–1338
    [Google Scholar]
  14. Malherbe H. H., Strickland-Cholmley M. 1967; Simian virus S. A. II. and the related O agent. Archiv für die gesamte Virusforschung 22:235–245
    [Google Scholar]
  15. Matsuno S., Inouye S., Kono R. 1977; Plaque assay of neonatal calf diarrhoea virus and the neutralizing antibody in human sera. Journal of Clinical Microbiology 5:1–4
    [Google Scholar]
  16. Mebus C. A., Underdahl N. R., Rhodes M. B., Twiehaus M. J. 1969; Calf diarrhoea (scours): reproduced with a virus from a field outbreak. University of Nebraska Agricultural Experiment Station Research Bulletin No. 233
    [Google Scholar]
  17. Newman J. F. E., Brown F., Bridger J. C., Woode G. N. 1975; Characterization of rotavirus. Nature, London 258:631–633
    [Google Scholar]
  18. Rodger S. M., Craven J. A., Williams I. 1975a; Demonstration of reovirus-like particles in intestinal contents of piglets with diarrhoea. Australian Veterinary Journal 51:536
    [Google Scholar]
  19. Rodger S. M., Schnagl R. D., Holmes I. H. 1975b; Biochemical and biophysical characteristics of diarrhoea virus of human and calf origin. Journal of Virology 16:1229–1235
    [Google Scholar]
  20. Rodger S. M., Schnagl R. D., Holmes I. H. 1977; Further biochemical characterization, including the detection of surface glycoproteins, of human, calf and simian rotaviruses. Journal of Virology 24:91–98
    [Google Scholar]
  21. Shapiro A. L., Vinuela E., Maizel J. V. Jun 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  22. Tzipori S., Caple I. W., Butler R. 1976; Isolation of a rotavirus from deer. Veterinary Record 99:398
    [Google Scholar]
  23. Zweerink H. J., McDowell M. J., Joklik W. K. 1971; Essential and nonessential noncapsid reovirus proteins. Virology 45:716–723
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-2-309
Loading
/content/journal/jgv/10.1099/0022-1317-43-2-309
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error