Plaque Formation by a Host Range Mutant of Vaccinia Virus in Non-permissive Cells Co-infected with Yaba Virus Free

Abstract

SUMMARY

A host-dependent conditional lethal mutant of vaccinia virus strain DIs was rescued to form plaques in a non-permissive cell line JINET co-infected with Yaba virus, a poxvirus serologically distant from the rescued virus. The efficiency of plaque formation under optimal conditions in this system was comparable to that in Vero cells which were permissive for the mutant. The plaque number of the mutant in JINET cells was influenced greatly by the multiplicity of Yaba virus, the interval between inoculations with DIs and Yaba virus and the maintenance medium for pre-infection of cells with Yaba virus. The plaque formation by DIs in JINET cells was suppressed or inhibited when the duration of pre-infection with high multiplicities of Yaba virus was prolonged. Thus, Yaba virus possessed both rescuing and inhibitory activities on DIs and the plaque number of DIs in doubly infected cells was thought to be determined by the balance between the two activities. Ultraviolet light-inactivated Yaba virus retained rescuing activity on DIs in JINET cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-1-193
1979-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/1/JV0430010193.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-1-193&mimeType=html&fmt=ahah

References

  1. Fenner F., Sambrook J. P. 1966; Conditional lethal mutants of rabbitpox virus. II. Mutants (p) that fail to multiply in PK-2a cells. Virology 28:600–609
    [Google Scholar]
  2. Joklik W. K., Woodroofe G. M., Holmes I. H., Fenner F. 1960; The reactivation of poxviruses. I. Demonstration of the phenomenon and techniques of assay. Virology u:168–184
    [Google Scholar]
  3. Mcclain W. E., Greenland R. M. 1965; Recombination between rabbitpox virus mutants in permissive and nonpermissive host cells. Virology 25:516–522
    [Google Scholar]
  4. Oda M. 1965; Rescue of dermavaccinia abortive infection by neurovaccinia virus in L cells. Virology 25:664–666
    [Google Scholar]
  5. Padgett B. L., Tomkins J. K. N. 1968; Conditional lethal mutants of rabbitpox virus. III. Temperature-sensitive (ts) mutants: Physiological properties, complementation and recombination. Virology 36:161–167
    [Google Scholar]
  6. Sambrook J. F., Mcclain M. E., Easterbrook K. B., Mcauslan B. R. 1965; A mutant of rabbitpOX virus defective at different stages of its multiplication in three cell types. Virology 26:738–745
    [Google Scholar]
  7. Sambrook J. F., Mcclain M. E., Easterbrook K. B., Mcauslan B. R. 1965; A mutant of TabbitpOX virus Isolation of host-dependent and temperature-dependent mutants. Virology 28:592–599
    [Google Scholar]
  8. Tagaya L., Kitamura T., Sano Y. 1961; A new mutant of vaccinia virus. Nature, London 192:381–382
    [Google Scholar]
  9. Tagaya I., Amano H., Kitamura T., Komatsu T., Ueda Y., Tanaka Y., Uchida N., Kodama H. 1972; Properties of an attenuated mutant of vaccinia virus, strain DIs. Symposium Series in Immunobiological Standardization 19:299–307
    [Google Scholar]
  10. Tsuchiya Y., Tagaya I. 1970; Enhanced or inhibited plaque formation of superinfecting viruses in Yaba virus-infected cells. Journal of General Virology 7:71–73
    [Google Scholar]
  11. Tsuchiya Y., Tagaya I. 1972a; General characteristics of enhanced plaque formation by poliovirus in poxvirus-infected cells. Journal of General Virology 14:229–235
    [Google Scholar]
  12. Tsuchiya Y., Tagaya I. 1972b; Mechanism of enhanced plaque formation by poliovirus in poxvirus-infected cells. Journal of General Virology 14:236–241
    [Google Scholar]
  13. Tsuchiya Y., Tagaya I. 1977; Rescue of host-dependent conditional lethal mutants of vaccinia and rabbitpox viruses by Yaba virus. Archives of Virology 55:341–345
    [Google Scholar]
  14. Tsuchiya Y., Tagaya L., Tsuruhara T. 1969; Titration and extensive serial passages of Yaba virus in vitro. Japanese Journal of Microbiology 13:103–117
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-1-193
Loading
/content/journal/jgv/10.1099/0022-1317-43-1-193
Loading

Data & Media loading...

Most cited Most Cited RSS feed