The Tryptic Peptides of the Coat Protein from Wild Type Cowpea Chlorotic Mottle Virus Free

Abstract

SUMMARY

The tryptic peptides of aminoethylated coat protein from cowpea chlorotic mottle virus were isolated and their amino acid compositions determined. Twenty-three major peptides accounted for 177 of the 182 amino acid residues which comprise the coat protein. The tryptic peptides were identified with respect to their position on two-dimensional peptide maps of cowpea chlorotic mottle virus protein, to aid future analysis of coat protein variants.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-43-1-143
1979-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/43/1/JV0430010143.html?itemId=/content/journal/jgv/10.1099/0022-1317-43-1-143&mimeType=html&fmt=ahah

References

  1. Bancroft J. B., Hiebert E., Rees M. W., Markham R. 1968; Properties of cowpea chlorotic mottle virus, its protein and nucleic acid. Virology 34:224–239
    [Google Scholar]
  2. Bancroft J. B., Mclean G. D., Rees M. W., Short M. N. 1971; The effect of an arginyl to a cysteinyl replacement on the uncoating behaviour of a spherical plant virus. Virology 45:707–715
    [Google Scholar]
  3. Bancroft J. B., Ress M. W., Dawson J. R. O., Mclean G. D., Short M. N. 1972; Some properties of a temperature-sensitive mutant of cowpea chlorotic mottle virus. Journal of General Virology 16:69–81
    [Google Scholar]
  4. Bancroft J. B., Rees M. W., Johnson M. W., Dawson J. R. O. 1973; A salt-stable mutant of cowpea chlorotic mottle virus. Journal of General Virology 21:507–513
    [Google Scholar]
  5. Bancroft J. B., McDonald J. G., Rees M. W. 1976; A mutant of cowpea chlorotic mottle virus with a perturbed assembly mechanism. Virology 75:293–305
    [Google Scholar]
  6. Dawson R. M. C., Elliot W. H., Elliot D. C., Jones K. M. 1959 In Data for Biochemical Research London and New York: Oxford University Press;
    [Google Scholar]
  7. Groskopf W. R., Holleman J. W., Margoliash E., Klotz I. M. 1966; Amino acid sequences of peptides from the tryptic digest of Goldfingia gouldii hemerythrin. Biochemistry 5:3783–3796
    [Google Scholar]
  8. Guest J. R., Carlton B. C., Yanofsky C. 1967; The amino acid sequence of the A protein (alpha subunit) of the tryptophan synthetase of Escherichia coli. 1. Tryptic peptides. Journal of Biological Chemistry 242:5397–5412
    [Google Scholar]
  9. Hayashi A., Stamatoyannopoulos G., Yoshida A., Adamson J. 1971; Haemoglobin Rainier: β 145 (HC2) Tyr → CysH and haemoglobin Bethesda /S145 (HC2) Tyr → His. Nature New Biology 230:264–267
    [Google Scholar]
  10. Irreverre F. 1965; A modified Sakaguchi spray. Biochimica et Biophysica Acta in:551–552
    [Google Scholar]
  11. Jepson J. B., Smith I. 1953; Multiple dipping procedures in paper chromatography. Nature, London 172:1100–1101
    [Google Scholar]
  12. Kimmel J. R., Kato G. K., Paiva A. C. M., Smith E. L. 1962; Tryptic digest of papain. I. Isolation and sequence of some peptides from the oxidised protein. Journal of Biological Chemistry 237:2525–2534
    [Google Scholar]
  13. Offord R. E. 1966; Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature, London 211:591–593
    [Google Scholar]
  14. Raftery M. A., Cole R. D. 1966; On the aminoethylation of proteins. Journal of Biological Chemistry 241:3457–3461
    [Google Scholar]
  15. Rees M. W., Short M. N., Kassanis B. 1970; The amino acid composition, antigenicity, and other characteristics of the satellite viruses of tobacco necrosis virus. Virology 40:448–461
    [Google Scholar]
  16. Reindel F., Hoppé W. 1954; A colour method for staining amino acids, peptides and proteins on paper-chromatograms and paper electrophoretograms. Chemische Berichte 87:1103–1107
    [Google Scholar]
  17. Smith I. 1953; Colour reactions on paper chromatograms by a dipping technique. Nature, London 171:43–44
    [Google Scholar]
  18. Spackman D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  19. Taniuchi H., Anfinsen C. B. 1966; The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. I. Linear order of the fragments produced by cleavage with cyanogen bromide. Journal of Biological Chemistry 241:4366–4385
    [Google Scholar]
  20. Taniuchi H., Anfinsen C. B., Sodja A. 1967; The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. II. The amino acid sequences of tryptic and chymotryptic peptides. Journal of Biological Chemistry 242:4736–4751
    [Google Scholar]
  21. Tremaine J. H., Agrawal H. O., Chidlow J. 1972; Partial sequence of the N-terminal portion of the protein of cowpea chlorotic mottle virus. Virology 48:245–254
    [Google Scholar]
  22. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  23. Yamada S., Itano H. A. 1966; Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines. Biochimica et Biophysica Acta 130:538–540
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-43-1-143
Loading
/content/journal/jgv/10.1099/0022-1317-43-1-143
Loading

Data & Media loading...

Most cited Most Cited RSS feed