Suppressive Effects of Interferon on Syncytium Formation by RD-114 Virus in Human Transformed Cells Free

Abstract

SUMMARY

The RD-114 virus rapidly induced syncytia in the human transformed cell lines, RSa, RSb and IF. Treatment of the virus with heat or ultrasonic vibration completely eliminated the syncytium-forming activity. Irradiation with u.v.-light or treatment with β-propiolactone (BPL) reduced but did not completely destroy the activity. Pre-treatment of the cells for 16 h with 25 to 500 units/ml of human leucocyte interferon (Le-IF) or fibroblast interferon (F-IF) significantly reduced formation of syncytia by active virus or inactivated (u.v. or BPL) virus. This activity of interferon was inhibited by treatment of the cells with cycloheximide. Interferon did not increase the binding of H-uridine-labelled RD-114 virus to the cells. It is postulated that interferon treatment altered the plasma membrane of the cells and thus reduced their capacity to fuse.

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1979-04-01
2024-03-28
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References

  1. Ahmed M., Korol W., Yeh J., Schidlovsky G., Mayyasi A. S. 1974; Detection of Mason-Pfizer virus infection with human KC cells carrying Rous virus genome. Journal of the National Cancer Institute 53:383–387
    [Google Scholar]
  2. Benveniste R. E., Todaro G. J. 1974; Evolution of C-type viral genes: inheritance of exogenously acquired viral genes. Nature, London 252:456–458
    [Google Scholar]
  3. Billiau A., Heremans H., Allen P. T., De Maeyer-Guignard J., De Somer P. 1976; Trapping of oncorna-virus particles at the surface of interferon-treated cells. Virology 73:537–542
    [Google Scholar]
  4. Bratt M. A., Gallaher W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proceedings of the National Academy of Sciences of the United States of America 64:536–543
    [Google Scholar]
  5. Chang H. E., Jay T. F., Friedman M. R. 1978; Physical, morphological, and biochemical alterations in the membrane of AKR mouse cells after interferon treatment. Proceedings of the National Academy of Sciences of the United States of America 75:1859–1863
    [Google Scholar]
  6. De Maeyer-Guignard J., Tovey M. G., Gresser I., De Mayer E. 1978; Purification of mouse interferon by sequential affinity chromatography on poly (U)- and antibody-agarose columns. Nature, London 271:622–625
    [Google Scholar]
  7. Fischinger P. H., Peebles P. T., Nomura S., Haapala D. K. 1973; Isolation of an RD-114-like oncornavirus from a cat cell line. Journal of Virology 11:978–985
    [Google Scholar]
  8. Friedman M. R. 1977; Antiviral activity of interferons. Bacteriological Reviews 41:543–567
    [Google Scholar]
  9. Gresser I. 1977a; Antitumor effects of interferon. In Cancer, A Comprehensive Treatise vol 5 pp 521–571 Edited by Becker F. New York: Plenum Press Inc;
    [Google Scholar]
  10. Gresser I. 1977b; On the varied biologic effects of interferon. Cellular Immunology 34:406–415
    [Google Scholar]
  11. Hampar L., Rand H. D., Lerner A. R., Del Villano C. B. Jun, McAllister R. M., Martos M. L., Derge G. J., Long W. C., Gilden V. R. 1973; Formation of syncytia in human lymphoblastoid cells infected with type C virus. Virology 55:453–463
    [Google Scholar]
  12. Johnson G. S., Friedman M. R., Pastan I. 1971; Analysis of the fusion of XC cells induced by homogenates of murine leukemia virus infected cells and by purified human leukemia virus. Journal of Virology 7:753–758
    [Google Scholar]
  13. Knight E. Jun, Korant D. B. 1977; A surface alteration in mouse L-cells induced by interferon. Biochemical and Biophysical Research Communications 74:707–713
    [Google Scholar]
  14. Kohn L. D., Friedman M. R., Holmes M. J., Lee G. 1976; Use of thyrotropin and chorela toxin to probe the mechanism by which interferon initiates its antiviral activity. Proceedings of the National Academy of Sciences of the United States of America 73:3695–3699
    [Google Scholar]
  15. Kuwata T., Oda T., Sekiya S., Morinaga N. 1976a; Characteristics of a human cell line successively transformed by Rous sarcoma virus and simian virus 40. Journal of the National Cancer Institute 56:919–926
    [Google Scholar]
  16. Kuwata T., Fuse A., Morinaga N. 1976b; Effects of interferon on ceil and virus growth in transformed human cell lines. Journal of General Virology 33:7–15
    [Google Scholar]
  17. Kuwata T., Fuse A., Morinaga N. 1977; Effects of cycloheximide and puromycin on the antiviral and anticellular activities of human interferon. Journal of General Virology 37:195–198
    [Google Scholar]
  18. Lindahl P., Leary P., Gresser I. 1973; Enhancement by interferon of the expression of surface antigens on murine leukemia L1210 cells. Proceedings of the National Academy of Sciences of the United States of America 70:2785–2788
    [Google Scholar]
  19. McAllister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma R. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V., Kabigting A., Vernon L. 1972; C-type virus released from cultured human rhabdomyosarcoma cells. Nature New Biology 235:3–6
    [Google Scholar]
  20. Moehring T. J., Moehring M. J., Stinebring R. W. 1971; Responses of interferon-treated cells to diphtheria toxin. Infection and Immunity 4:742–752
    [Google Scholar]
  21. Ogura H., Tanaka T., Ocho M., Kuwata T., Oda T. 1978; Detection of Mason-Pfizer monkey virus infection by syncytia formation of human cells doubly transformed by Rous sarcoma virus and simian virus 40. Archives of Virology 57:195–198
    [Google Scholar]
  22. Okabe H., Gilden R. V., Hatanaka M. 1973; RD-114 virus: extensive homology with RNA of feline cell origin. Nature New Biology 244:54–56
    [Google Scholar]
  23. Paucker K., Cantell K., Henle W. 1962; Quantitative studies on viral interference in suspended L cells. III. Effect of interfering virus and interferon on the growth of cells. Virology 17:324–334
    [Google Scholar]
  24. Pitha P. A., Rowe W. P., Oxman M. N. 1976; Effect of interferon on exogenous, endogenous, and chronic murine leukemia virus infection. Virology 70:324–338
    [Google Scholar]
  25. Rand H. K., Long W. C. 1972; Syncytial assay for the putative human C-type virus, RD-114, utilizing human cells transformed by Rous sarcoma virus. Nature New Biology 240:187–190
    [Google Scholar]
  26. Rand H. K., Long W. C. 1973; Fusion of a Rous sarcoma virus transformed human cell line, KC, by RD-114 virus. Journal of General Virology 21:523–532
    [Google Scholar]
  27. Rand H. K., Long W. C., Wei T. T., Gilden N. R. 1974; Plaque assay for the Mason-Pfizer monkey virus. Journal of the National Cancer Institute 53:449–452
    [Google Scholar]
  28. Rand K., Davis J., Oroszlan S., Long C. 1975; Fusion inhibition: bioassay of a type C viral protein. Virology 64:63–74
    [Google Scholar]
  29. Stewart T. W. II, De Clercq E., Billiau A., Desmyter J., De Somer P. 1972; Increased susceptibility of cells treated with interferon to the toxicity of poly-riboinosinic.poly-ribocytidylic acid. Proceedings of the National Academy of Sciences of the United States of America 69:1851–1854
    [Google Scholar]
  30. Tomita Y., Kuwata T. 1976; Effects of adriamycin on the reverse transcriptase and the production of murine leukemia virus. Cancer Research 36:3016–3019
    [Google Scholar]
  31. Tomita Y., Kuwata T. 1978; Suppression of murine leukemia virus production by ouabain and interferon in mouse cells. Journal of General Virology 38:223–230
    [Google Scholar]
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