1887

Abstract

SUMMARY

Immunoprecipitates obtained by reacting Dane particle cores with human antibodies to hepatitis B core antigen (HBcAg) were chromatographed on columns of Sepharose 4B CL using 3 -NaSCN as eluant. An antigen having the size and immunological specificity of monomeric e-antigen (HBeAg) was separated from HBcAg by this method. Antisera from animals immunized with HBcAg or HBeAg reacted not only with the antigen used for immunization but also with HBeAg and HBcAg, respectively. This indicates that HBeAg determinants are associated with the core of Dane particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-42-3-645
1979-03-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/42/3/JV0420030645.html?itemId=/content/journal/jgv/10.1099/0022-1317-42-3-645&mimeType=html&fmt=ahah

References

  1. Alter H. J., Seeff L. B., Kaplan P. M., McAuliffe V. J., Wright E. C., Gerin J. L., Purcell R. H., Holland P. V., Zimmerman J. H. 1976; Type B hepatitis: the infectivity of blood positive for e antigen and DNA polymerase after accidental needlestick exposure. New England Journal of Medicine 295:909–913
    [Google Scholar]
  2. Arnold W., Jsielsen J. O., Hardt F., Meyer Zum Buschenfelde K. J. 1977; Localisation of e-antigen in nuclei of hepatocytes in HBsAg-positive liver diseases. Gut 19:994–996
    [Google Scholar]
  3. Beasley R. P., Trepo C., Stevens C. E., Szmuness W. 1977; The e antigen and vertical transmission of hepatitis B surface antigen. American Journal of Epidemiology 105:94–98
    [Google Scholar]
  4. Biddison W. E., Doherty P. C., Webster R. G. 1977; Antibody to influenza virus matrix protein detects a common antigen on the surface of cells infected with Type A influenza viruses. Journal of Experimental Medicine 146:690–697
    [Google Scholar]
  5. Braciale T. J. 1977; Immunologic recognition of influenza virus-infected cells. II. Expression of influenza A matrix protein on the infected cell surface and its role in recognition by cross-reactive cytotoxic T cells. Journal of Experimental Medicine 146:673–689
    [Google Scholar]
  6. Budkowska A. 1977; Immunochemical and morphological studies of hepatitis B core antigen isolated from the nuclei of hepatocytes. Journal of Infectious Diseases 135:463–467
    [Google Scholar]
  7. Fields H. A., Hollinger F. B., Desmyter J., Melnick J. L., Dreesman G. R. 1977; Biochemical and biophysical properties of hepatitis B core particles derived from Dane particles and infected hepatocytes. Intervirology 8:336–350
    [Google Scholar]
  8. Gerin J. L., Shih J. W. K., McAuliffe V. J., Purcell R. H. 1978; Antigens of hepatitis B virus: failure to detect HBeAg on the surfaces of HBsAg forms. Journal of General Virology 38:561–566
    [Google Scholar]
  9. Howard C. R., Burrell C. J. 1976; Structure and nature of hepatitis B antigen. Progress in Medical Virology 22:36–103
    [Google Scholar]
  10. Huang S. N., Neurath A. R. 1978; Immunohistologic demonstration of hepatitis B viral antigens in liver with reference to its significance in liver injury. Laboratory Investigation (in the press)
    [Google Scholar]
  11. Lam K. C., Tong M. J., Rakela J. 1977; Release of e antigen from a Dane particle-rich preparation of hepatitis B virus. Infection and Immunity 16:403–404
    [Google Scholar]
  12. Ledbetter J., Nowinski R. C., Emery S. 1977; Viral proteins expressed on the surface of murine leukemia cells. Journal of Virology 22:65–73
    [Google Scholar]
  13. Murphy S. L., Peterson J. M., Smith J. L., Gitnick G. L., Auslander G. O., Berquist V. R., Maynard D. E., Purcell R. H. 1976; Correlation between fluorescent antibody detection of hepatitis B core antigen in liver biopsies and the presence of e-antigen in the serum. Infection and Immunity 13:296–297
    [Google Scholar]
  14. Mushahwar I. K., Overby L. R., Frosner G., Deinhardt F., Ling C.-M. 1978; Prevalence of hepatitis B e antigen and its antibody as detected by radioimmunoassays. Journal of Medical Virology 2:77–87
    [Google Scholar]
  15. Neurath A. R., Trepo C., Chen M., Prince A. M. 1976; Identification of additional antigenic sites on Dane particles and the tubular forms of hepatitis B surface antigen. Journal of General Virology 30:277–285
    [Google Scholar]
  16. Neurath A. R., Huang S. N., Strick N. 1978a; Some properties of hepatitis B core antigen isolated from serum of infected humans. Journal of General Virology 39:91–101
    [Google Scholar]
  17. Neurath A. R., Szmuness W., Stevens C. E., Strick N., Harley E. J. 1978b; Radioimmunoassay and some properties of human antibodies to hepatitis B core antigen. Journal of General Virology 38:549–559
    [Google Scholar]
  18. Neurath A. R., Strick N., Szmuness W., Stevens C. E., Harley E. J. 1978c; Radioimmunoassay of hepatitis B e-antigen (HBeAg): identification of HBeAg not associated with immunoglobulins. Journal of General Virology 42: (in the press)
    [Google Scholar]
  19. Nordenfelt E., Kjellen L. 1975; Dane particles, DNA polymerase, and e-antigen in two different categories of hepatitis B antigen carriers. Intervirology 5:225–232
    [Google Scholar]
  20. Okada K., Kamiyama I., Inomata M., Imai M., Miyakawa Y., Mayumi M. 1976; e antigen and anti-e in the serum of asymptomatic carrier mothers as indicators of positive and negative transmission of hepatitis B virus to their infants. New England Journal of Medicine 294:746–749
    [Google Scholar]
  21. Purcell R. H., Gerin J. L., Almeida J. B., Holland P. V. 1974; Radioimmunoassay for the detection of the core of the Dane particle and antibody to it. Intervirology 2:231–243
    [Google Scholar]
  22. Shikata T., Karasawa T., Abe K., Uzawa T., Suzuki H., Oda T., Imai M., Mayumi M., Moritsugu Y. 1977; Hepatitis B e antigen and infectivity of hepatitis B virus. Journal of Infectious Diseases 136:571–576
    [Google Scholar]
  23. Takahashi K., Imai M., Tsuda F., Takahashi T., Miyakawa Y., Mayumi M. 1976; Association of Dane particles with e antigen in the serum of asymptomatic carriers of hepatitis B surface antigen. Journal of Immunology 117:102–105
    [Google Scholar]
  24. Takahashi S., Yamashita K., Imai M., Miyakawa Y., Mayumi M. 1978; Failure of antibody to e antigen to precipitate Dane particles containing DNA polymerase activity and hepatitis B core antigen. Journal of General Virology 38:431–436
    [Google Scholar]
  25. Trepo C. G., Magnius L. O., Schaefer R. A., Prince A. M. 1976; Detection of e antigen and antibody. Correlations with hepatitis B surface and hepatitis B core antigens, liver disease, and outcome of hepatitis B infections. Gastroenterology 71:804–808
    [Google Scholar]
  26. Tung J. S., Yoshiki T., Fleissner E. 1976; A core polyprotein of murine leukemia virus on the surface of mouse leukemia cells. Cell 9:573–578
    [Google Scholar]
  27. Virelizier J. L., Allison A. C., Oxford J. S., Schild G. C. 1977; Early presence of ribonucleoprotein antigen on surface of influenza virus-infected cells. Nature, London 266:52–54
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-42-3-645
Loading
/content/journal/jgv/10.1099/0022-1317-42-3-645
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error