A persistent infection with rabies virus (HEP-Flury) was established in the CNS-derived hybrid cell line 108 CC15 which possesses specific membrane receptors for prostaglandins, catecholamines and acetylcholine. We report a differential virus influence on the specific receptor response to PGE, isoproterenol and acetycholine as indicated by typical changes of the intracellular cyclic AMP levels.
As the adenylate cyclase activity was unchanged in infected cells in vitro, a selective virus influence on specific receptors themselves or their coupling to the cAMP synthesizing system must be considered.
GilmanA. G.1970; A protein binding assay for adenosine 3′:5′-cyclic monophosphate. Proceedings of the National Academy of Sciences of the United States of America 67:305–312
HalbachM.,
KoschelK.1979; Impairment of hormone dependent signal transfer by chronic SSPE-virus infection. Journal of General Virology 42: (previous paper)
HamprechtB.1974; Cell cultures as model systems for studying the biochemistry of differentiated functions of nerve cells. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie 355:109–110
HamprechtB.1977; Structural, electrophysiological, biochemical and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. International Review of Cytology 49:99–170
HamprechtB.,
SchultzJ.1973; Influence of noradrenalin, prostaglandin E1 and inhibitors of phosphodiesterase activity on levels of cyclic adenosine 3′:5’-monophosphate in somatic cell hybrids. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie 354:1633–1641
HeumannR.,
ValetG.,
MaisonD.,
KemperJ.,
ReiserG.,
HamprechtB.1977; Influence of the time in culture on cellular and neuronal properties of neuroblastoma × glioma hybrid cells. With an appendix-Mathematical description of the kinetics of the loss in cell volume. Journal of Cell Science 27:141–155
HollandJ. J.,
VillarrealL. P.,
WelshR. M.,
OldstoneM. B. A.,
KohneD.,
LazzariniR.,
ScolnickE.1976; Long-term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. Journal of General Virology 33:193–211
PalmaE. L.,
HuangA. S.1974; Cyclic production of vesicular stomatitis virus caused by defective interfering particles. Journal of Infectious Diseases 129:402–410
PeytremanA.,
NicholsonW. E.,
LiddleA. W.,
HartmanJ. G.,
SutherlandE. W.1973; Effects of methylxanthines on adenosine 3′: 5′-monophosphate and corticosterone in the rat adrenal. Endocrinology 92:525–530
SharmaS. K.,
NirenbergM.,
KleeW. A.1975; Morphine receptors as regulators of adenylate cyclase activity. Proceedings of the National Academy of Sciences of the United States of America 72:590–594
TraberJ.,
ReiserG.,
FischerK.,
HamprechtB.1975b; Measurement of adenosine 3′: 5′ cyclic monophosphate and membrane potential in neuroblastoma × glioma hybrid cells: opiates and adrenergic agonists cause effects opposite to those of prostaglandin. FEBS Letters 52:327–332
WeissM. C.,
EphrussiB.1966; Studies of interspecific (rat × mouse) somatic hybrids. I. Isolation, growth and evolution of the karyotype. Genetics 54:1095–1109