1887

Abstract

SUMMARY

The effects of cell metabolic activity on the outcome of influenza virus infection were studied in partially synchronized chick embryo fibroblast cultures. There was no evidence to show that the time in the cell cycle at which cells were infected had any significant effect on the final virus yield. However, some differences were detected in the length of the latent period between infections established in synchronized or in stationary cells. Influenza virus could replicate in synchronized or normal cell cultures in which DNA synthesis was inhibited with 9-β--arabinofuranosyladenine (ara-A).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-42-3-579
1979-03-01
2022-09-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/42/3/JV0420030579.html?itemId=/content/journal/jgv/10.1099/0022-1317-42-3-579&mimeType=html&fmt=ahah

References

  1. Armstrong S. J., Barry R. D. 1974; The topography of RNA synthesis in cells infected with fowl plague virus. Journal of General Virology 24:535–547
    [Google Scholar]
  2. Bader J. P. 1965; The requirement for DNA synthesis in the growth of Rous sarcoma and Rous-associated viruses. Virology 26:253–261
    [Google Scholar]
  3. Barry R. D. 1964; The effects of actinomycin D and ultraviolet irradiation on the production of fowl plague virus. Virology 24:563–569
    [Google Scholar]
  4. Barry R. D., Ives D. R., Cruickshank J. G. 1962; Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature, London 194:1139–1140
    [Google Scholar]
  5. Borland R., Mahy B. W. J. 1968; DNA-dependent RNA polymerase activity in cells infected with influenza virus. Journal of Virology 2:33–39
    [Google Scholar]
  6. Buthala D. A. 1964; Cell culture studies on antiviral agents. 1. Action of cytosine arabinoside and some comparisons with 5-iodo-2-deoxyuridine. Proceedings of the Society for Experimental Biology and Medicine 115:69–77
    [Google Scholar]
  7. Cheyne I. M., White D. O. 1969; Growth of paramyxoviruses in anucleate cells. Australian Journal of Experimental Biology and Medical Science 47:145–147
    [Google Scholar]
  8. Cox N. J., O’Neill M. C., Kendal A. P. 1977; Replication of animal viruses in differentiating muscle cells: influenza virus A. Journal of General Virology 37:161–173
    [Google Scholar]
  9. Follett E. A. C., Pringle C. R., Wunner W. H., Skehel J. J. 1974; Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus. Journal of Virology 13:394–399
    [Google Scholar]
  10. Humphries E. H., Temin H. M. 1974; Requirement for cell division for initiation of transcription of Rous sarcoma virus. Journal of Virology 14:531–546
    [Google Scholar]
  11. Johnson L. F., Abelson H. T., Green H., Penman S. 1974; Changes in RNA in relation to growth of the fibroblast. 1. Amounts of mRNA, rRNA and tRNA in resting and growing cells. Cell 1:95–100
    [Google Scholar]
  12. Kelly D. C., Avery R. J., Dimmock N. J. 1974; Failure of an influenza virus to initiate infection in enucleate cells. Journal of Virology 13:1155–1161
    [Google Scholar]
  13. Lamb R. A., Choppin P. W. 1977; Synthesis of influenza virus polypeptides in cells resistant to alpha- amanitin: evidence for the involvement of cellular RNA polymerase II in virus replication. Journal of Virology 23:816–819
    [Google Scholar]
  14. Lomniczi B., Bosch F. X., Hay A. J., Skehel J. J. 1977; Influenza virus infection of a cell-cycle mutant of Chinese hamster ovary cells. Journal of General Virology 35:187–190
    [Google Scholar]
  15. Long W. F., Cooper R. J. 1974; Infection of synchronised cells by influenza virus. Microbios 10A:45–53
    [Google Scholar]
  16. Mahy B. W. J., Hastie N. D., Armstrong S. J. 1972; Inhibition of influenza virus replication by α-amanitin: mode of action. Proceedings of the National Academy of Sciences of the United States of America 69:1421–1424
    [Google Scholar]
  17. Mitchison J. M. 1969; Enzyme synthesis in synchronous cultures. Science 165:657–663
    [Google Scholar]
  18. Moore G. P. M., Ringertz N. R. 1973; Localization of DNA dependent RNA polymerase activities in fixed human fibroblasts by autoradiography. Experimental Cell Research 76:223–228
    [Google Scholar]
  19. Nayak D. P., Rasmussen A. F. Jun 1966; Influence of mitomycin C on the replication of influenza viruses. Virology 30:673–683
    [Google Scholar]
  20. O’Neill M. C., Kendal A. P. 1975; Infection of differentiating muscle cells with influenza and Newcastle disease virus. Nature, London 253:195–198
    [Google Scholar]
  21. Panem S., Schauf V. 1974; Cell cycle dependent appearance of murine leukaemia-sarcoma virus antigens. Journal of Virology 13:1169–1175
    [Google Scholar]
  22. Pringle C. R. 1977; Enucleation as a technique in the study of virus-host interactions. Current Topics in Microbiology and Immunology 76:49–82
    [Google Scholar]
  23. Rott R., Saber S., Scholtissek C. 1965; Effect on myxovirus of mitomycin C, actinomycin D, and pre-treatment of the host cell with ultraviolet light. Nature, London 205:1187–1190
    [Google Scholar]
  24. Rott R., Scholtissek C. 1970; Specific inhibition of influenza replication by α-amanitin. Nature, London 228:56–57
    [Google Scholar]
  25. Scholtissek C., Rott R. 1961; Zusammenhange zwischen der synthese von Ribonucleinsaure und Protein bei der Vermehrung eines Virus der Influenza-Gruppe (Virus der Klassischen Geflugelpest). Zeitschrift für Naturforschung 166:663–673
    [Google Scholar]
  26. Spooner L. L. R., Barry R. D. 1977; Participation of DNA-dependent RNA polymerase II in the replication of influenza viruses. Nature, London 268:650–652
    [Google Scholar]
  27. Stambrook P. J., Sisken J. E. 1972; The relationship between rates of 3H uridine and 3H adenine incorporation into RNA and the measured rates of RNA synthesis during the cell cycle. Biochimica et Biophysica Acta 281:45–54
    [Google Scholar]
  28. Temin H. M. 1967; Studies on carcinogenesis by avian sarcoma viruses. V. Requirement for new DNA synthesis and for cell division. Journal of Cellular Physiology 69:53–64
    [Google Scholar]
  29. Ward G. A., Plagemann P. G. W. 1969; Fluctuations of DNA dependent RNA polymerase and synthesis of macromolecules during the growth cycle of Novikoff rat hepatoma cells in suspension culture. Journal of Cellular Physiology 73:213–231
    [Google Scholar]
  30. White D. O., Day H. M., Batchelder E. J., Cheyne L. M., Wansbrough A. J. 1965; Delay in the multiplication of influenza virus. Virology 25:289–302
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-42-3-579
Loading
/content/journal/jgv/10.1099/0022-1317-42-3-579
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error