Rapid Diagnosis of Tick-borne Encephalitis by means of Enzyme Linked Immunosorbent Assay Free

Abstract

SUMMARY

An enzyme-linked immunosorbent assay was applied for determining separately IgM and IgG antibodies against tick-borne encephalitis virus. A micro-modification in microtitre plates proved to be at least as sensitive as the HI test. However, more precise information could be achieved by a macrotest using antigen coated polystyrene balls. False positive results in IgM antibody determinations could be caused by a rheumatoid factor. A high content of IgM antibodies in a serum could impair the determination of its IgG antibodies but not vice versa. Titres were expressed in comparison to a positive control serum.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-42-3-505
1979-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/42/3/JV0420030505.html?itemId=/content/journal/jgv/10.1099/0022-1317-42-3-505&mimeType=html&fmt=ahah

References

  1. Bid well D. E., Bartlett A., Voller A. 1977; Enzyme immunoassays for viral diseases. Journal of Infectious Diseases 316: (supplement) 274–278
    [Google Scholar]
  2. Castellano G. A., Hazzard G. T., Madden D. L., Sever J. L. 1977; Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination test for detection of antibody to cytomegalovirus. Journal of Infectious Diseases 136: (supplement) 337–34
    [Google Scholar]
  3. Delia S., Russo V., Vollo V., Aceti A., Ferone U. 1977; Determination of specific antibodies to influenza by ELISA. Lancet 1:346
    [Google Scholar]
  4. Frisch-Niggemeyer W. 1975; Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections. Journal of Clinical Microbiology 2:377–381
    [Google Scholar]
  5. Frisch-niggemeyer W., Halonen P., Heinz E., Kunz Ch., Ziola B. 1978; Festphasen-Radioimmunoassay zum Nachweis klassenspezifischer Immunoglobuline gegen das Virus Frűhsommer-Meningoen-zephalomyelitis. Immunitat und Infektion 6:110–117
    [Google Scholar]
  6. Gilman S. C., Docherty J. J. 1977; Detection of antibodies specific for herpes simplex virus in human sera by the enzyme-linked immunosorbent assay. Journal of Infectious Diseases 136: (supplement) 286–293
    [Google Scholar]
  7. Gravell M., Dorsett P. H., Gutenson O., Ley A. C. 1977; Detection of antibody to rubella virus by enzyme-linked immunosorbent assay. Journal of Infectious Diseases 136: (supplement) 300–303
    [Google Scholar]
  8. Heinz F., Kunz Ch. 1977; Concentration and purification of tick-borne encephalitis virus grown in suspensions of chick embryo cells. Acta Virologica 21:301–307
    [Google Scholar]
  9. Kunz Ch., Hofmann H. 1971; Die friihdiagnose der fruhsommermeningoezephalitis (FSME) imhamagglutinationshermmungstest durch behandlung des serums mit 2-mercaptoathanol. Zentralblatt fur Bakteriologie, Hygiene und Prevantivmedizin, I. Abteilung A Originate 218:373–379
    [Google Scholar]
  10. Leinikki P. O., Passila S. 1977; Quantitative semiautomated, enzyme-linked immunosorbent assay for viral antibodies. Journal of Infectious Diseases 136: (supplement) 249–299
    [Google Scholar]
  11. Lowry O. H., Rosenbrough H. J., Farr A. L., Randall R. J. 1951; Protein measurement with thefolin phenol reagent. Journal of Biological Chemistry 193:265–271
    [Google Scholar]
  12. Reimer C. B., Black C. M., Phillips D. J., Logan L. C., Hunter E. F., Pender B. J., Mcgrew B. E. 1975; The specificity of fetal IgM: antibody or anti-antibody. Annals of the New York Academy of Sciences 254:77–93
    [Google Scholar]
  13. Robertson P. W., Kersetz V., Cloonan M. J. 1977; Elimination of false-positive cytomegalovirus immungloblin M-fluorescent-antibody reactions with immunoglobulin M serum fractions. Journal of Clinical Microbiology 5:174–175
    [Google Scholar]
  14. Saunders G. C., Clinard E. H., Bartlett M. L., Sanders W. M. 1977; Application of the indirect enzyme-labelled antibody microtest to the detection and surveillance of animal diseases. Journal of Infectious Diseases 136:258–266
    [Google Scholar]
  15. Voller A., Bid well D. E. 1975; A simple method for detecting antibodies to rubella. British Journal of Experimental Pathology 56:338–339
    [Google Scholar]
  16. Voller A., Bid well D. E. 1976; Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. British Journal of Experimental Pathology 57:243–247
    [Google Scholar]
  17. Voller A., Bidwell D. E., Bartlett A. 1976; Enzyme immunoassays in diagnostic medicine. Theory and practice. Bulletin of the World Health Organization 53:55–56
    [Google Scholar]
  18. Wallen W. C., Mattson J. M., Levine P. H. 1977; Detection of soluble antigen of Epstein-Barr virus by the enzyme-linked immunosorbent assay. Journal of Infectious Diseases 136: (supplement) 324–328
    [Google Scholar]
  19. Ziola B. R., Mattkainen M. T., Salmi A. 1977; Polystyrene balls as the solid-phase of a double-antibody radioimmunoassay for human serum albumin. Journal of Immunological Methods 17:309–317
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-42-3-505
Loading
/content/journal/jgv/10.1099/0022-1317-42-3-505
Loading

Data & Media loading...

Most cited Most Cited RSS feed