1887

Abstract

SUMMARY

A radioimmunoassay for hepatitis B e-antigen (HBeAg) is described. Polystyrene beads coated with IgG prepared from a human serum containing antibodies to HBeAg (anti-HBe) and anti-HBe IgG labelled with I--hydroxyphenylpropionic acid -hydroxysuccinimide ester were used in the test. The radioimmunoassay was approximately 1000-fold more sensitive than immunodiffusion. At least a transient presence of HBeAg in serum appears to be a common feature of infections by hepatitis B virus. The radioimmunoassay was instrumental in establishing conditions for identification of apparently free monomeric HBeAg. The HBeAg has an approximate mol. wt. of 35000 and was recovered after isoelectric focusing in fractions with a pH between 4.25 and 4.8. Polyacrylamide gel electrophoresis revealed the presence in HBeAg of a polypeptide with an apparent mol. wt. of 17000.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-42-3-493
1979-03-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/42/3/JV0420030493.html?itemId=/content/journal/jgv/10.1099/0022-1317-42-3-493&mimeType=html&fmt=ahah

References

  1. Aikawa T., Sairenji H., Furuta S., Kiyosawa K., Shikata T., Imai M., Miyakawa Y., Yanase Y., Mayumi M. 1978; Seroconversion from hepatitis B e antigen to anti-HBe in acute hepatitis B virus infection. New England Journal of Medicine 298:439–441
    [Google Scholar]
  2. Alter H. J., Seeff L. B., Kaplan P. M., Mcauliffe V. J., Wright E. C., Gerin J. L., Purcell R. H., Holland P. V., Zimmerman J. H. 1976; Type B hepatitis: The infectivity of blood positive for e antigen and DNA polymerase after accidental needlestick exposure. New England Journal of Medicine 295:909–913
    [Google Scholar]
  3. Andrews P. 1964; Estimation of the molecular weights of proteins by Sephadex gel-filtrations. Biochemical Journal 91:222–233
    [Google Scholar]
  4. Beasley R. P., Trepo C., Stevens C. E., Szmuness W. 1977; The e antigen and vertical transmission of hepatitis B surface antigen. American Journal of Epidemiology 105:94–98
    [Google Scholar]
  5. Biswas R., Gerlich W., Thomssen R. 1978; Comparison of radioimmunoassay and immunodiffusion in the detection of HBeAg and anti-HBe. In Viral Hepatitis Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  6. Fields H. A., Bradley D. W., Davis C. L., Maynard J. E. 1978; Radioimmunoassay for the detection of hepatitis e antigen (HBeAg) and antibody (anti-HBe). Abstracts of the Annual Meeting of the American Society for Microbiology47
    [Google Scholar]
  7. Froessner G. G., Deinhardt F. W., Overby R. L., Papaevangelou G., Brodersen M. 1978; Prevalence of HBeAg and anti-HBe by solid phase radioimmunoassay in acute hepatitis B. In Viral Hepatitis Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  8. Fudenberg H. H. 1967 In Methods in Immunology and Immunochemistry vol 1 chapter 3 pp 307–385 Edited by Williams C. A., Chase M. W. New York: Academic Press;
    [Google Scholar]
  9. Grady G. F. 1976; Relation of e antigen to infectivity of HBsAg-positive inoculations among medical personnel. Lancet 11:492–494
    [Google Scholar]
  10. Iwata T., Iwata H., Holland J. F. 1968; Isolation of albumin from human serum by means of trichloroacetic acid and ethanol. A comparison of methods. Clinical Chemistry 14:22–30
    [Google Scholar]
  11. Kacaki J. N., Siem T. H., Brouwer R. 1977; Anti-e and rheumatoid factor activity in hepatitis B. Lancet i:1008–1009
    [Google Scholar]
  12. Klaus G. G. B. 1978; Antigen-antibody complexes elicit anti-idiotypic antibodies to self-idiotypes. Nature, London 272:265–266
    [Google Scholar]
  13. Magnius L. O., Espmark S. A. 1972; New specifications in Australia antigen positive sera distinct from LeBouvier determininations. Journal of Immunology 109:1017–1021
    [Google Scholar]
  14. Matuhasi T., Hosokawa Z. 1972; Reactants to human serum albumin-coated red cells found in Au(i)-positive sera. Japanese Journal of Experimental Medicine 42:183–185
    [Google Scholar]
  15. McAuliffe V. I. 1978 In Viral Hepatitis Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  16. Miyakawa Y., Mayumi M. 1978 In Viral Hepatitis Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  17. Mushahwar I. K., Ling C. M., Overby L. R. 1978; Studies on HBeAg and anti-HBe utilizing radioactive assay techniques. In Viral Hepatitis Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  18. Neurath A. R. 1978; Structure of HBeAg. In Viral Hepatitis Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  19. Neurath A. R., Huang S. N., Strick N. 1978a; Some properties of hepatitis B core antigen isolated from serum of infected humans. Journal of General Virology 39:91–101
    [Google Scholar]
  20. Neurath A. R., Prince A. M., Giacalone J. 1978b; Large-scale purification of hepatitis B surface antigen using affinity chromatography. Experientia 37:414–415
    [Google Scholar]
  21. Neurath A. R., Rubin B. A. 1971 In Viral Structural Components as Immunogens of Prophylactic Value. Monographs in Virology vol 4 pp 1–37 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  22. Neurath A. R., Strick N. 1977; Host specificity of a serum marker for hepatitis B: evidence that ‘e antigen’ has the properties of an immunoglobulin. Proceedings of the National Academy of Sciences of the United States of America 74:1702–1706
    [Google Scholar]
  23. Neurath A. R., Szmuness W., Stevens C. E., Strick N., Harley E. J. 1978c; Radioimmunoassay and some properties of human antibodies to hepatitis B core antigen. Journal of General Virology 38:549–559
    [Google Scholar]
  24. Neurath A. R., Trepo C., Chen M., Prince A. M. 1976; Identification of additional antigenic sites on Dane particles and the tubular form of hepatitis B surface antigen. Journal of General Virology 30:277–285 (1976)
    [Google Scholar]
  25. NordenfelT E., Kjellen L. 1975; Dane particles, DNA polymerase, and e-antigen in two different categories of hepatitis B antigen carriers. Intervirology 5:225–232
    [Google Scholar]
  26. Okado K., Kamiyama I., Inomata M., Imai M., Miyakawa Y., Mayumi M. 1976; e antigen and anti-e in the serum of asymptomatic carrier mothers as indicators of positive and negative transmission of hepatitis B virus to their infants. New England Journal of Medicine 294:746–749
    [Google Scholar]
  27. Shikata T., Karasawa T., Abe K., Uzawa T., Suzuki H., Oda T., Imai M., Mayumi M., Moritsugu Y. 1977; Hepatitis B e antigen and infectivity of hepatitis B virus. Journal of Infectious Diseases 136:571–576
    [Google Scholar]
  28. Takahashi K., Fukuda M., Baba M., Imai K., Miyakawa Y., Mayumi M. 1977; Determination of e antigen and antibody to e by means of passive hemagglutination method. Journal of Immunology 119:1556–1559
    [Google Scholar]
  29. Takahashi K., Imai M., Miyakawa Y., Iwakiri S., Mayumi M. 1978; Duality of hepatitis B e antigen in serum of persons infected with hepatitis B virus: evidence for the nonidentity of e antigen with immunoglobulins. Proceedings of the National Academy of Sciences of the United States of Americans1952–1956
    [Google Scholar]
  30. Takahashi K., Imai M., Tsuda F., Takahashi T., Miyakawa Y., Mayumi M. 1976; Association of Dane particles with e antigen in the serum of asymptomatic carriers of hepatitis B surface antigen. Journal of Immunology 117:102–105
    [Google Scholar]
  31. Tedder R. S. 1978 In Viral Hepatitis Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  32. Tedder R. S., Briggs M. 1977; Anti-e and rheumatoid factor activity in hepatitis B. Lancet i:1262–1263
    [Google Scholar]
  33. Tong M. J., Stevenson D., Gordon I. 1977; Correlation of e antigen, DNA polymerase activity, and Dane particles in chronic benign and chronic active type B hepatitis infections. Journal of Infectious Diseases 1 35:980–984
    [Google Scholar]
  34. Werner B. G., O’Connell A. P., Summers J. 1977; Association of e antigen with Dane particle DNA in sera from asymptomatic carriers of hepatitis B surface antigen. Proceedings of the National Academy of Sciences of the United States of America 74:2149–2151
    [Google Scholar]
  35. Williams A., Lebouvier G. 1976; HBs Antigen Subtypes. In Bibliotheca Haematologica vol 42 pp 71–75 Edited by Hassig H. Basel: S. Karger;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-42-3-493
Loading
/content/journal/jgv/10.1099/0022-1317-42-3-493
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error