1887

Abstract

SUMMARY

Interferon (150 units/ml) was used to treat SC-1 and AKR-2B cells which were chronically infected with murine leukaemia virus (MuLV). This led to a 100-fold decrease in the amount of infectious virus released into the medium and a 10-fold decrease in the number of virus particles measured by the virion-associated reverse transcriptase assay. However, there was little change in the amount of cell-associated infectious virus, though nearly twice as many cell-associated virions were counted in electron micrographs. With both types of cells, interferon blocked MuLV replication at the post-budding stage, but it did not change the morphology of the particles produced or their content of virion 70S RNA.

Infectious virus assembled on the cell membranes of interferon-treated cells was less stable at 37°C than that grown in the absence of interferon. Release of infectious virus from interferon-treated cells was not inhibited by actinomycin D or cycloheximide, though both agents inhibited virus production in controls.

These results show that interferon inhibits MuLV replication through effects on virion assembly; these lead both to the formation of non-infectious particles and of fewer virions. Kinetic analysis further shows that interferon affects MuLV assembly rapidly and induction of an antiviral protein may not be required.

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1979-03-01
2022-01-16
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References

  1. Aboud M., Shoor R., Salzberg S. 1978; Effect of interferon action on exogenous murine leukemia virus infection. Virology 84:134–141
    [Google Scholar]
  2. Billiau A., Sorbis H., Desomer P. 1973; Influence of interferon on virus particle synthesis in oncornavirus carrier line: evidence for a direct effect on particle release. International Journal of Cancer 1 2:646–653
    [Google Scholar]
  3. Billiau A., Edy V. S., Sorbis H., Desomer P. 1974; Influence of interferon on virus-particle synthesis in oncornavirus-carrier cell lines. II. Evidence for a direct effect on particle release. International Journal of Cancer 14:335–340
    [Google Scholar]
  4. Billiau A., Edy V. G., Declerco E., Heremans H., Desomer P. 1975; Influence of interferon on the synthesis of virus particles in oncornavirus-carrier cell lines. III. Survey of effects on A-, B- and C- type oncornaviruses. International Journal of Cancer 15:947–953
    [Google Scholar]
  5. Billiau A., Heremans H., Allen P. T., Demaeyer-Guignard J., Desomer P. 1976a; Trapping of OnCOT-navirus particles at the surface of interferon-treated cells. Virology 73:537–542
    [Google Scholar]
  6. Billiau A., Heremans H., Allen P. T., Desomer P. 1976b; Influence of interferon on the synthesis of virus particles in oncornavirus-carrier cell lines. IV. Relevance to the potential application of interferon in natural infectious diseases. Journal of Infectious Diseases 133: (Suppl.) A54–A55
    [Google Scholar]
  7. Chang E. H., Mims S. J., Triche T. J., Friedman R. M. 1977; Interferon inhibits mouse leukemia virus release: an electron microscopic study. Journal of General Virology 34:363–367
    [Google Scholar]
  8. DeHarven E., Lampen N., Sato T. 1973; Scanning electron microscopy of cells infected with a murine leukemia virus. Journal of Virology 15:240–243
    [Google Scholar]
  9. Duesberg P. H., Robinson H. L., Robinson W. S., Huebenr R. J., Turner H. C. 1968; Proteins of ROUS sarcoma virus. Virology 36:73–86
    [Google Scholar]
  10. Friedman R. M., Chang E. H., Ramseur J. M., Myers M. W. 1975; Interferon-directed inhibition of chronic murine leukemia virus production in cell cultures: lack of effect on intracellular viral markers. Journal of Virology 16:569–574
    [Google Scholar]
  11. Friedman R. M., Ramseur J. M. 1974; Inhibition of murine leukemia virus production in chronically infected AKR cells: a novel effect of interferon. Proceedings of the National Academy of Sciences of the United States of America 71:3542–3544
    [Google Scholar]
  12. Hartley J. W., Rowe W. P. 1975; Clonal cell lines from a feral mouse embryo which lack host range restrictions for murine leukemia viruses. Virology 65:128–134
    [Google Scholar]
  13. Hunter E., Hayman M. J., Rongey R. W., Vogt P. K. 1976; An avian sarcoma virus mutant that is temperature sensitive for virion assembly. Virology 69:35–49
    [Google Scholar]
  14. Knight E. 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  15. Levin J. G., Grimley P. M., Ramseur J. M., Berezesky I. K. 1974; Deficiency of 60 to 70S RNA in murine leukemia virus particles assembled in cells treated with actinomycin D. Journal of Virology 14:152–161
    [Google Scholar]
  16. Levin J. G., Rosenak M. J. 1976; Synthesis of murine leukemia virus proteins associated with virions assembled in actinomycin D-treated cells: evidence for persistence of viral messenger RNA. Proceedings of the National Academy of Sciences of the United States of America 73:1154–1158
    [Google Scholar]
  17. Luftig R. B., Concience J. F., Skoreltchi A., Mcmiltiau P., Revel M., Ruddle F. 1977; Effect of interferon on dimethyl sulfoxide stimulated Friend erythroleukemic cells: ultrastructural and biochemical study. Journal of Virology 23:799–810
    [Google Scholar]
  18. Malech H. L., Wivel N. A. 1976; Properties of murine intracisternal A particles. Electron microscopic appearance after critical point drying and platinum shadowing. Virology 69:802–809
    [Google Scholar]
  19. Morris A. G., Clegg C. 1978; The effect of mouse interferon on the transformation of NIH/3T3 mouse cells by murine sarcoma virus. Virology 88:400–402
    [Google Scholar]
  20. Palmiter R. D. 1974; Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acid. Biochemistry 17:3606–3615
    [Google Scholar]
  21. Panem S., Kirsten W. H. 1975; Secondary scanning electron microscopy of cells infected with murine oncornaviruses. Virology 63:447–458
    [Google Scholar]
  22. Pitha P. M., Rowe W. P., Oxman M. N. 1976; Effect of interferon on exogenous, endogenous, and chronic murine leukemia virus infection. Virology 70:324–338
    [Google Scholar]
  23. Pitha P. M., Staal S. P., Bolognesi D. P., Denny T. P., Rowe W. P. 1977; Effect of interferon on murine leukemia virus infection. II. Synthesis of viral components in exogenous infection. Virology 79:1–13
    [Google Scholar]
  24. Pitha P. M., Staal S. P. 1978; Effect of interferon on murine leukemia virus infection. III. Effect of interferon on MSV induced transformation. Virology 90:151–155
    [Google Scholar]
  25. Rowe W. P., Hartley J. W., Lander M. R., Pugh W. E., Teich N. 1971; Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology 46:866–867
    [Google Scholar]
  26. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  27. Svoboda J., Chyle P. P., Simkovic D., Hilgert T. 1963; Demonstration of the absence of infectious Rous virus in rat tumor XC, whose structurally intact cells produce Rous sarcoma when transferred to chick. Folia Biologica Prague 9:77–81
    [Google Scholar]
  28. Swetly P., Ostertag W. 1974; Friend virus release and induction of hemoglobin synthesis in ery-throleukemia cells respond differently to interferon. Nature, London 251:642–644
    [Google Scholar]
  29. Taylor J. 1965; Studies on the mechanism of action of interferon. Interferon action and RNA synthesis in chick embryo fibroblasts infected with Semliki Forest virus. Virology 25:340–349
    [Google Scholar]
  30. Van Griensven L. J. D. L., Baudelaire M. C., Peries J., Em Anoel-R A Vicovitch R., Boiron M. 1971 In Biology of Oncogenic Viruses vol 2 pp 145–153 Lepetit Colloquia on Biology and Medicine Amsterdam: North-Holland;
    [Google Scholar]
  31. Wivel N. A., Smith G. H. 1971; Distribution of intracisternal A-particles in a variety of normal and neoplastic mouse tissues. International Journal of Cancer 7:167–175
    [Google Scholar]
  32. Wong P. K. Y., Carter J. A. 1974; Studies of two temperature sensitive mutants of Moloney murine leukemia virus. Virology 58:396–408
    [Google Scholar]
  33. Wong P. K. Y., Macleod R. 1975; Studies on the budding process of a temperature-sensitive mutant of murine leukemia virus with a scanning electron microscope. Journal of Virology 16:434–442
    [Google Scholar]
  34. Wong P. K. Y., Yuen P. H., Macleod R., Chang E. H., Myers M. W., Friedman R. M. 1977; The effect of interferon on de novo infection of Moloney murine leukemia virus. Cell 10:245–252
    [Google Scholar]
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