1887

Abstract

SUMMARY

The proteins synthesized in protoplasts infected with strain V5 of brome mosaic virus have been studied. Four new proteins of mol.wt. 2, 3.5, 10 and 10.7 × 10 were observed. These account for over 90% of the virus genome. The three proteins with lowest mol.wt. corresponded closely in size to those observed in protoplasts infected with cowpea chlorotic mottle virus.

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/content/journal/jgv/10.1099/0022-1317-42-2-323
1979-02-01
2024-12-05
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References

  1. Bancroft J. B., Flack I. H. 1972; The behaviour of cowpea chlorotic mottle virus in CsCl. Journal of General Virology 15:247–251
    [Google Scholar]
  2. Bancroft J. B., Lane L. C. 1973; Genetic analysis of cowpea chlorotic mottle and brome mosaic viruses. Journal of General Virology 19:381–389
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Davies J. W., Kaesberg P. 1974; Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ. Journal of General Virology 25:11–20
    [Google Scholar]
  5. Goodman D., Matzura H. 1971; An improved method of counting radioactive acrylamide gels. Analytical Biochemistry 42:481–486
    [Google Scholar]
  6. Hariharasubramanian V., Hadidi A., Singer B., Fraenkel-Bonrat H. 1973; Possible identification of a protein in brome mosaic virus infected barley as a component of viral RNA polymerase. Virology 54:190–198
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Lane L. C. 1974; The bromoviruses. Advances in Virus Research 19:151–220
    [Google Scholar]
  9. Lane L. C., Kaesberg P. 1971; Multiple genetic components in bromegrass mosaic virus. Nature New Biology 232:40–43
    [Google Scholar]
  10. Laskey R. A., Mills A. D. 1975; Qualitative film detection of 3H and 14C in polyacrylamide gels by fluoro-graphy. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  11. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  12. Motoyoshi F., Bancroft J. B., Watts J. W. 1974a; The infection of tobacco protoplasts with a variant of brome mosaic virus. Journal of General Virology 25:31–36
    [Google Scholar]
  13. Motoyoshi F., Watts I. W., Bancroft J. B. 1974b; Factors influencing the infection of tobacco protoplasts by cowpea chlorotic mottle virus. Journal of General Virology 25:245–256
    [Google Scholar]
  14. Paterson R., Knight C. A. 1975; Protein synthesis in tobacco protoplasts infected with tobacco mosaic virus. Virology 64:10–22
    [Google Scholar]
  15. Sakai F., Takebe I. 1972; A non-coat protein synthesized in tobacco mesophyll protoplasts infected by tobacco mosaic virus. Molecular and General Genetics 118:93–96
    [Google Scholar]
  16. Sakai F., Takebe I. 1974; Protein synthesis in tobacco mesophyll protoplasts induced by tobacco mosaic virus infection. Virology 62:426–433
    [Google Scholar]
  17. Sakai F., Watts J. W., Dawson J. R. O., Bancroft I. B. 1977; Synthesis of proteins in tobacco protoplasts infected with cowpea chlorotic mottle virus. Journal of General Virology 34:285–293
    [Google Scholar]
  18. Shih D. S., Kaesberg P. 1973; Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo. Proceedings of the National Academy of Sciences of the United States of America 70:1799–1803
    [Google Scholar]
  19. Shih D. S., Kaesberg P. 1976; Translation of the RNAs of brome mosaic virus: the monocistronic nature of RNA 1 and RNA 2. Journal of Molecular Biology 103:77–88
    [Google Scholar]
  20. Watts J. W., Sakai F., King J. 1977; A simple technique for autoradiography of cylindrical polyacrylamide gels. Analytical Biochemistry 79:579–582
    [Google Scholar]
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