@article{mbs:/content/journal/jgv/10.1099/0022-1317-42-2-297, author = "Borden, Ernest C. and Booth, Bruce W. and McBain, Jack A.", title = "Enhancement of Infectivity of Encephalomyocarditis Virus RNA by Amphotericin B Methyl Ester", journal= "Journal of General Virology", year = "1979", volume = "42", number = "2", pages = "297-303", doi = "https://doi.org/10.1099/0022-1317-42-2-297", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-42-2-297", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY The methyl ester of amphotericin B (AmBME), a macrolide polyene antibiotic, enhanced the infectivity of encephalomyocarditis (EMC) virus RNA for L929 cells. AmBME alone (100 µg/ml) resulted in increases in EMC virus RNA infectivity of 10- to 100-fold. Addition of DEAE dextran at concentrations (5 µg/ml), which alone slightly suppressed EMC virus RNA infectivity, further augmented the effects of AmBME (augmentation in infectivity up to 750-fold). AmBME did not inhibit RNase, did not enhance EMC virus infectivity and increased infectivity of EMC virus RNA which was already cell-associated. The polyenes are probably acting by increasing intracellular penetration of polyribonucleotides.", }