1887

Abstract

SUMMARY

Exposure of transmissible gastroenteritis virus of pigs to unheated normal heterotypic serum resulted in a drop in infectivity, an effect which was lost after heating the serum to 56 °C for 30 min or by treatments inactivating complement. Analysis of virus proteins, RNA and lipids, and centrifugation studies showed little difference between inactivated and control virus, but electron microscopy of negatively stained particles after treatment with serum revealed holes in the virus envelope, characteristic of those caused by complement in the presence of antibody.

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/content/journal/jgv/10.1099/0022-1317-42-2-279
1979-02-01
2022-01-21
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References

  1. Andrewes C. H., Elford W. J. 1933; Observations on anti-phage sera. 1. ′The percentage law′. British Journal of Experimental Pathology 14:367–376
    [Google Scholar]
  2. Apostolov K., Sawa M. I. 1976; Enhancement of haemolysis by Newcastle Disease virus (NDV) after pre-treatment with heterophile antibody and complement. Journal of General Virology 33:459–469
    [Google Scholar]
  3. Berry D. M., Almeida J. D. 1968; The morphological and biological effects of various antisera on avian infectious bronchitis virus. Journal of General Virology 3:97–102
    [Google Scholar]
  4. Binn L. N., Lazar E. C., Keenan K. P., Huxsoll D. L., Marchwicki B. S., Strand A. J. 1974; Recovery and characterisation of a coronavirus from military dogs with diarrhoea. Proceedings of the 78th Annual Meeting U.S. Animal Health Association359–366
    [Google Scholar]
  5. Bradstreet C. M., Taylor C. E. D. 1962; Technique of complement-fixation test applicable to the diagnosis of virus diseases. Monthly Bulletin of the Ministry of Health and the Public Health Laboratory Service 21:96–104
    [Google Scholar]
  6. Derbyshire J. B., Collins A. P. 1972; An experimental epidemiological study of Talfan virus infection in pigs. Journal of Comparative Pathology 82:315–322
    [Google Scholar]
  7. Fine D. P., Marney S. R. Jun., Colley D. J., Sergent J. S., Des Prez R. M. 1972; C3 shunt activation in human serum chelated with EGTA. Journal of Immunology 109:807–809
    [Google Scholar]
  8. Garwes D. J., Pocock D. H. 1975; The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29:25–34
    [Google Scholar]
  9. Garwes D. J., Pocock D. H., Pike B. V. 1976; Isolation of subviral components from transmissible gastroenteritis virus. Journal of General Virology 32:283–294
    [Google Scholar]
  10. Garwes D. J., Pocock D. H., Wijaszka T. M. 1975; Identification of heat-dissociable RNA complexes in two porcine coronaviruses. Nature, London 257:508–510
    [Google Scholar]
  11. Gregory D. W., Pirie B. J. S. 1973; Wetting agents for biological electron microscopy. 1. General considerations and negative staining. Journal of Microscopy 99:261–265
    [Google Scholar]
  12. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194:495–496
    [Google Scholar]
  13. Linscott W. D., Levinson W. E. 1969; Complement components required for virus neutralization by early immunoglobulin antibody. Proceedings of the National Academy of Sciences of the United States of America 64:520–527
    [Google Scholar]
  14. Pike B. V., Garwes D. J. 1977; The lipids of transmissible gastroenteritis virus and their relation to those of two different host cells. Journal of General Virology 34:531–535
    [Google Scholar]
  15. Pocock D. H., Garwes D. J. 1975; The influence of pH on the growth and stability of transmissible gastroenteritis virus in vitro. Archives of Virology 49:239–247
    [Google Scholar]
  16. Reynolds D. J., Garwes D. J., Gaskell C. I. 1977; Detection of transmissible gastroenteritis virus neutralizing antibody in cats. Archives of Virology 55:77–86
    [Google Scholar]
  17. Shilo M. 1959; Nonspecific resistance to infections. Annual Review of Microbiology 13:255–278
    [Google Scholar]
  18. Skarnes R. C., Watson D. W. 1957; Antimicrobial factors of normal tissues and fluids. Bacteriological Reviews 21:273–294
    [Google Scholar]
  19. Svehag S.-E., Mandel B. 1964; The formation and properties of poliovirus neutralizing antibody, 1. 19S and 7S antibody formation: differences in kinetics and antigen dose requirement for induction. Journal of Experimental Medicine 119:1–19
    [Google Scholar]
  20. Toussaint A. I. 1960; Bacteriophage neutralization by serum. Federation Proceedings 19:412
    [Google Scholar]
  21. Welsh R. M., Cooper N. R., Jensen F. C., Oldstone M. B. A. 1975; Human serum lyses RNA tumor viruses. Nature, London 257:612–614
    [Google Scholar]
  22. Welsh R. M., Jensen F. C., Cooper N. R., Oldstone M. B. A. 1976; Inactivation and lysis of oncornaviruses by human serum. Virology 74:432–440
    [Google Scholar]
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