1887

Abstract

SUMMARY

Highly purified preparations of the isometric mushroom viruses 1 and 4 were made by a combination of differential centrifugation, ion-exchange chromatography on DEAE-cellulose and sucrose density-gradient centrifugation. Purified preparations of MV1 virions (25 nm diam.) sedimented at 130S contained one capsid polypeptide of mol. wt. 24400 and two species of double-stranded RNA of mol. wt. approx. 1.4 × 10. MV4 particles (35 nm diam.) had a sedimentation value of 140 to 145S, contained one capsid polypeptide of mol. wt. 63800 and two species of dsRNA of mol. wt. 1.5 × 10 and 1.4 × 10. MV1 and MV4 were antigenically unrelated.

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1979-02-01
2024-04-23
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References

  1. Albaum H. G., Umbreit W. W. 1947; Differentiation between ribose 3-phosphate and ribose 5-phosphate by means of the orcinol-pentose reaction. Journal of Biological Chemistry 167:369–376
    [Google Scholar]
  2. Atkey P. T., Barton R. J., Hollings M., Stone O. M. 1974; Mushroom (Agaricus bisporus). Report of Glasshouse Crops Research Institute 1973: pp 119–120
    [Google Scholar]
  3. Barton R. J. 1977; An examination of permeation chromatography on columns of controlled pore glass for routine purification of plant viruses. Journal of General Virology 35:77–87
    [Google Scholar]
  4. Bozarth R. F., Wood H. A., Mandelbrot A. 1971; The Penicillium stoloniferum virus complex: two similar double stranded RNA virus-like particles in a single cell. Virology 45:516–523
    [Google Scholar]
  5. Buck K. W., Kempson-Jones G. F. 1973; Biophysical properties of Penicillium stoloniferum vims S. Journal of General Virology 18:223–235
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and of the mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
    [Google Scholar]
  7. Cowan K. M., Graves J. H. 1968; Immunochemical studies of foot-and-mouth disease. III. Acridine orange staining of agar gel precipitin reactions. Virology 34:544–548
    [Google Scholar]
  8. Dieleman-Van Zaayen A., Temmink J. H. M. 1968; A virus disease of cultivated mushrooms in the Netherlands. Netherlands Journal of Plant Pathology 74:48–51
    [Google Scholar]
  9. Diener T. O., Schneider I. R. 1968; Virus degradation and nucleic acid release in single phase phenol systems. Archives of Biochemistry and Biophysics 124:401–412
    [Google Scholar]
  10. Francki R. I. B., Jackson A. O. 1972; Immunochemical detection of double-stranded ribonucleic acid in leaves of sugar cane infected with Fiji disease. Virology 48:275–277
    [Google Scholar]
  11. Fujii-Kawata I., Miura K., Fuke M. 1970; Segments of genome of viruses containing double-stranded ribonucleic acid. Journal of Molecular Biology 51:247–253
    [Google Scholar]
  12. Holungs M. 1962; Viruses associated with a die-back disease of cultivated mushroom. Nature, London 196:962–965
    [Google Scholar]
  13. Holungs M., Stone O. M. 1971; Viruses that infect fungi. Annual Review of Phytopathology 9:93–118
    [Google Scholar]
  14. Hollings M., Stone O. M., Atkey P. T. 1968; Mushroom viruses. Report of the Glasshouse Crops Research Institute 1967:101–102
    [Google Scholar]
  15. Holungs M., Stone O. M., Atkey P. T. 1971; Mushrooms. Report of the Glass House Crops Research Institute 1970:157–159
    [Google Scholar]
  16. Lapierre H., Molin G., Faivre-Amiot A., Michon E., Albouy J., Morand J. C. 1971; Présence departicules à symétrie hélicoidale dans une souche du champignon de couche cultive dans le nord de la France. Annales de Phytopathologie 3:538–539
    [Google Scholar]
  17. Last F. T., Hollings M., Stone O. M. 1974; Effects of cultural conditions on the mycelial growth of healthy and virus-infected cultivated mushrooms, Agaricus bisporus. Annals of Applied Biology 76:99–111
    [Google Scholar]
  18. Layne E. 1957; Spectrophotometric and turbimetric methods for measuring protein. In Methods in Enzymology vol 3 pp 447–454 New York: Academic Press;
    [Google Scholar]
  19. Lemke P. A., Nash C. H. 1974; Fungal viruses. Bacteriological Reviews 38:29–56
    [Google Scholar]
  20. Loening U. E. 1968; Molecular weights of ribosomal RNA in relation to evolution. Journal of Molecular Biology 38:355–365
    [Google Scholar]
  21. Luisoni E. 1972; Virus-like particles in cultivated mushrooms in Italy. Rivista di Patologia Vegetale, Serie IV 8:199–206
    [Google Scholar]
  22. Marino R., Saksena K. N., Schuler M., Mayfield J. E., Lemke P. A. 1976; Double-stranded ribonucleic acid in Agaricus bisporus. Applied and Environmental Microbiology 31:433–438
    [Google Scholar]
  23. Molin G., Lapierre H. 1973; L’acide nucleique des virus de champignons: cas des virus de l’Agaricus bisporus. Annales de Phytopathologie 5:233–240
    [Google Scholar]
  24. Paul H. L. 1959; Die bestimmung des nucleinsauregehalte pflanzlicher viren mit hilfe einer spektrophoto-metrischen methode. Zeitschrift für Naturforschung 14:427–432
    [Google Scholar]
  25. Shapiro A. L., Vinuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  26. Weber K., Osborne M. 1969; Reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide-gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  27. Wood H. A., Bozarth R. F. 1972; Properties of virus-like particles of Penicillium chrysogenum: one doublestranded RNA molecule per particle. Virology 47604–609
    [Google Scholar]
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