A study of the physical properties of the β and ε viruses showed the ε virus to be slightly larger but less dense in CsCl than the β virus even though no difference in size or shape could be detected by electron microscopy. Comparative values for the physical properties of the β and ε viruses respectively were: sedimentation coefficient 213 and 217S, diffusion coefficient 0.97 and 0.79 × 10 cm/s, buoyant density in CsCl 1.30 and 1.28 g/ml, and diam. in the electron microscope 39 to 40 nm for both viruses. All preparations of β virus included small amounts of β virus antigen with a buoyant density of 1.33 g/ml.


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