1887

Abstract

SUMMARY

The human papova (JC) virus was extracted from brain of a patient with progressive multifocal leukoencephalopathy. A single band of virus was obtained at a density of 1.345 g/ml CsCl. JC virus DNA was purified and a highly specific cRNA was generated hybridization with JC virus cRNA and autoradiography on sections of the same brain revealed silver grains over oligodendrocytes, astrocytes and possible vascular endothelial cells, indicating the presence of JC virus DNA in these different cell classes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-42-1-49
1979-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/42/1/JV0420010049.html?itemId=/content/journal/jgv/10.1099/0022-1317-42-1-49&mimeType=html&fmt=ahah

References

  1. Aström K. E., Mancall E. L., Richardson E. P. 1958; Progressive multifocal leukoencephalopathy. A hitherto unrecognized complication of chronic lymphatic leukemia and Hodgkin’s disease. Brain 81:93–111
    [Google Scholar]
  2. Burgess R. R. 1969; A new method for the large-scale purification of Escherichia coli deoxyribonucleic acid dependent ribonucleic acid polymerase. Journal of Biological Chemistry 244:6160–6165
    [Google Scholar]
  3. Castaigne P., Rondot P., Escourolle R., Ribadeau-Dumas J. L., Cathala F., Hauw J. J. 1974; Leucoencéphalopathie multifocale progressive et ‘gliomes’ multiples. Revue Neurologique, Paris 130:379–392
    [Google Scholar]
  4. Fareed G., Takemoto K., Gimbrone M. 1978; Interaction of human papovaviruses, JC and BK with human vascular endothelium. Microbiology (in the press)
    [Google Scholar]
  5. Huang E.-S., Pagano J. S. 1977; Nucleic acid hybridization technology and detection of proviral genomes. In Methods in Virology vol VI chapter 13 pp 457–497 Edited by Maramorosch K., Koprowski H. New York and London: Academic Press;
    [Google Scholar]
  6. Miquel J., Calvo W., Rubinstein L. J. 1968; A simple and rapid stain for the biopsy diagnosis of brain tumors. Journal of Neuropathology and Experimental Neurology 27:517–523
    [Google Scholar]
  7. Narayan O. 1976; On the neoplastic viral transformation of astrocytes in cases of progressive multifocal leukoencephalopathy. Journal of Neuropathology and Experimental Neurology 35:313
    [Google Scholar]
  8. Padgett B. L., Walker D. L., Zu Rhein G. M., Hodach A. E., Chou S. M. 1976; JC papovavirus in progressive multifocal leukoencephalopathy. Journal of Infectious Diseases 133:686–690
    [Google Scholar]
  9. Penny J. B., Weiner L. P., Herndon R. M., Narayan O., Johnson R. T. 1972; Virions from progressive multifocal leucoencephalopathy: rapid serological identification by electronmicroscopy. Science 178:60–62
    [Google Scholar]
  10. Richardson E. P. 1961; Progressive multifocal leucoencephalopathy. New England Journal of Medicine 265:815–823
    [Google Scholar]
  11. Shein H. M. 1967; Transformation of astrocytes and destruction of spongioblasts induced by a simian tumour virus (SV40) in cultures of human fetal glia. Journal of Neuropathology and Experimental Neurology 26:60–76
    [Google Scholar]
  12. Tai H. T., Smith C. A., Sharp P. A., Vinograd J. 1972; Sequence heterogeneity in closed simian virus 40 deoxyribonucleic acid. Journal of Virology 9:317–325
    [Google Scholar]
  13. Weiner L. P., Narayan O., Penny J. B. Jun, Herndon M., Feringa E. R., Tourtellotte W. W., Johnson R. T. 1973; Papovavirus of JC type in progressive multifocal leucoencephalopathy. Archives of Neurology 29:1–3
    [Google Scholar]
  14. Wolf H., Zur Hausen H., Becker V. 1973; EB viral genomes in epithelial nasopharyngeal carcinoma cells. Nature New Biology 244:245–247
    [Google Scholar]
  15. Zur Hausen H., Diehl V., Wolf H., Schulte-Holthausen H., Schneider U. 1972; Occurrence of EB viral genomes in human lymphoblastoid cell lines. Nature, London 237:189–190
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-42-1-49
Loading
/content/journal/jgv/10.1099/0022-1317-42-1-49
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error