1887

Abstract

SUMMARY

Analysis by SDS-polyacrylamide gel electrophoresis shows that the purified coronavirus JHM contains six polypeptides. The apparent mol. wt. of the polypeptides (GP1, GP2, GP3, VP4, GP5 and VP6) are 170000; 125000; 97500; 60800; 24800 and 22700, respectively. Four polypeptides are glycosylated (GP1, GP2, GP3 and GP5). The analysis of particles obtained after limited proteolysis with pronase suggests that GP2 and GP3 are protruding from the lipid envelope and, together with GP1, form the spike layer. Protein VP6 and a part of GP5 are located within the lipid bilayer. Protein VP4 is susceptible to digestion at a concentration of pronase which changes the morphology of the virus particles making the interior of the virus accessible. Subviral particles produced after treatment with the detergent Nonidet P40 banded at a higher density than the virus and contained only VP4, GP5 and VP6.

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1979-01-01
2022-10-03
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References

  1. Bingham R. W. 1975; The polypeptide composition of avian infectious bronchitis virus. Archives of Virology 49:207–216
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Collins M. S., Alexander D. J., Harkness J. W. 1976; Heterogeneity of infectious bronchitis virus grown in eggs. Archives of Virology 50:55–72
    [Google Scholar]
  4. Garwes D. J., Pocock D. H. 1975; The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29:25–34
    [Google Scholar]
  5. Garwes D. J., Pocock D. H., Pike B. V. 1976; Isolation of subviral components from transmissible gastroenteritis virus. Journal of General Virology 32:283–294
    [Google Scholar]
  6. Hierholzer J. C. 1976; Purification and biophysical properties of human coronavirus 229E. Virology 75:I55–165
    [Google Scholar]
  7. Hierholzer J. C., Palmer E. L., Whitfield S. G., Kaye H. S., Dowdle W. R. 1972; Protein composition of coronavirus OC43. Virology 48:516–527
    [Google Scholar]
  8. Kennedy D. A., Johnson-Lussenburg C. M. 1976; Isolation and morphology of the internal component of human coronavirus strain 229E. Intervirology 6:197–206
    [Google Scholar]
  9. Laemmli U. I. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. MacNauohton M. R., Madge H. M. 1977; The polypeptide composition of avian infectious bronchitis virus particles. Archives of Virology 55:47–54
    [Google Scholar]
  11. MacNaughton M. R., Madge H. M., Davies H. A., Dourmashkin R. R. 1977; Polypeptides of the Surface projections and the ribonucleoprotein of avian infectious bronchitis virus. Journal of Virology 24:821–825
    [Google Scholar]
  12. McIntosh K. 1974; Coronaviruses: a comparative review. Current Topics in Microbiology and Immunology 63:85–129
    [Google Scholar]
  13. Nagashima K., Wege H., Ter Meulen V. 1978; Early and late CNS-effects of coronavirus infection in rats. Advances in Experimental Medicine and Biology 100:395–409
    [Google Scholar]
  14. Pocock D. H., Garwes D. J. 1977; The polypeptides of haemagglutinating encephalomyelitis virus and isolated subvirual particles. Journal of General Virology 37:487–499
    [Google Scholar]
  15. Sturman L. S. 1977; Characterization of a coronavirus I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels. Virology 77:637–649
    [Google Scholar]
  16. Sturman L. S., Holmes K. V. 1977; Characterization of a coronavirus II. Glycoproteins of the viral envelope: tryptic peptide analysis. Virology 77:650–660
    [Google Scholar]
  17. Tyrrell D. A. J., Almeida J. D., Cunningham C. H., Dowdle W. R., Hofstad M. S., McIntosh K., Tajima M., Zakstelskaya L. Ya., Easterday B. C., Kapikian A., Bingham R. W. 1975; Coronaviridae. Intervirology 5:76–82
    [Google Scholar]
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