1887

Abstract

SUMMARY

Infectious virus nucleic acids were extracted from the Hirt supernatant of chicken embryo fibroblasts infected for different times with the Schmidt-Ruppin strain of Rous sarcoma virus (RSV). Infectious DNA and DNA-RNA hybrid molecules could be recovered from 6 h after infection in experiments using 1.5 × 10 infected cells. Only small amounts of infectious virus DNA could be purified 6 h after infection whereas at 24 h approximately one infectious DNA molecule could be recovered for each input virus infectious unit. At 24 h, both infectious supercoiled and non-supercoiled molecules were found. The specific infectivity of the supercoiled fraction was less than that of the non-supercoiled fraction. Infectious supercoiled DNA could be recovered from 16 h after infection. Evidence is presented that both forms of unintegrated virus DNA may rest unintegrated for at least 8 days in the cell, though chronically infected cells were shown to contain less than one unintegrated molecule per 10 to 10 cells.

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1979-01-01
2024-03-28
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References

  1. Canaani E., Duesberg P. H. 1972; Role of subunits of 60–70S avian tumour virus RNA in its template activity for viral polymerase. Journal of Virology 10:23–31
    [Google Scholar]
  2. Commerford S. L. 1971; Iodination of nucleic acids in vitro. Biochemistry 10:1993–1999
    [Google Scholar]
  3. Fritsch E., Temin H. M. 1977; Formation and structure of infectious DNA of spleen necrosis virus. Journal of Virology 21:119–130
    [Google Scholar]
  4. Graham F. L., van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  5. Guntaka R. V., Richards O. C., Shank P. R., Rung H. J., Davidson N., Fritsch E., Bishop J. M., Varmus H. E. 1976; Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected quail tumor cells and measurement by electron microscopy and gel electrophoresis. Journal of Molecular Biology 106:337–357
    [Google Scholar]
  6. Hill M., Hillova J. 1976; Genetic transformation lf animal cells with viral DNA of RNA tumor viruses. Advances in Cancer Research 23:237–297
    [Google Scholar]
  7. Hill M., Stedman N., Mariage R., Goubin G., Hillova J., Roussel M. 1978; Unintegrated and integrated viral DNA in Rous sarcoma virus-infected chicken cells. In Avian RNA Tumor Viruses pp 155–177 Edited by Barlati S., de Giuli-Morghen C. Padua: Piccin Editore;
    [Google Scholar]
  8. Hillova J., Dantchev D., Mariage R., Plichon M. P., Hill M. 1974; Sarcoma and transformation-defective viruses produced with infectious DNA(s) from Rous sarcoma virus (RSV)-transformed chicken cells. Virology 62:197–208
    [Google Scholar]
  9. Hillova J., Hill M., Goubin G., Dantchev D. 1975; Infectivity of Rous sarcoma cell DNA: comparison of two techniques of transfection assay. Intervirology 5:367–374
    [Google Scholar]
  10. Hillova J., Hill M., Kalekine M. 1976; Inability of the non-defective Rous sarcoma provirus to generate, upon transfection, a transformation-defective virus. Virology 74:540–543
    [Google Scholar]
  11. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  12. Junghans R. P., Hu S., Knight C. A., Davidson N. 1977; Heteroduplex analysis of avian RNA tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 74:477–481
    [Google Scholar]
  13. Khoury A. T., Hanafusa H. 1976; Synthesis and integration of viral DNA in chicken cells at different times after infection with various multiplicities of avian oncornavirus. Journal of Virology 18:383–400
    [Google Scholar]
  14. Leis J., Schincariol A., Ishizaki R., Hurwitz J. 1975; RNA-dependent DNA polymerase activity of RNA tumor viruses. V. Rous sarcoma virus single-stranded RNA-DNA covalent hybrids in infected chicken embryo fibroblast cells. Journal of Virology 15:484–489
    [Google Scholar]
  15. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  16. Murphy H. M. 1977; A new replication-defective variant of the Bryan high-titer strain Rous sarcoma virus. Virology 77:705–721
    [Google Scholar]
  17. Ono K., Stedman N. R., Dantchev D., Hillova J., Hill M. 1976; Endogenous DNA polymerase of a transformation-defective Rous sarcoma virus: characterization and comparison with the enzyme of the non-defective parent. Biomedicine 24:237–248
    [Google Scholar]
  18. Radloff R., Bauer W., Vinograd J. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57:1514–1521
    [Google Scholar]
  19. Rosenthal L. J., Zamecnik P. C. 1973; Minor base composition of ‘7oS-associated’ 4S RNA from avian myeloblastosis virus. Proceedings of the National Academy of Sciences of the United States of America 70:865–869
    [Google Scholar]
  20. Beotkin D., Gianni A. M., Rozenblatt S., Weinberg R. A. 1975; Infectious viral DNA of murine leukemia virus. Proceedings of the National Academy of Sciences of the United States of America 72:4910–4913
    [Google Scholar]
  21. Somsr H. A. 1970 In Handbook of Biochemistry. Selected Data for Molecular Biology 2nd edition Cleveland, OHIO: CRC PRESS.;
    [Google Scholar]
  22. Sveda M. M., Fields B. N., Soeiro R. 1974 Host restriction of Friend leukemia virus: fate of input virion RNA. Cell 2:271–277
    [Google Scholar]
  23. Śveda M. M., Fields B. N., Soeiro R. 1976; Fate of input oncomavirion RNA – biological studies. Journal of Virology 18:85–91
    [Google Scholar]
  24. Takano T., Hatanaka M. 1975; Fate of viral RNA of murine leukemia virus after infection. Proceedings of the National Academy of Sciences of the United States of America 72:343–347
    [Google Scholar]
  25. Taylor J. M. 1977; An analysis of the role of RNA species as primers for the transcription into DNA of RNA tumor virus genomes. Biochimica et Biophysica Acta 473:57–71
    [Google Scholar]
  26. Varmus H. E., Shank P. R. 1976; Unintegrated viral DNA is synthesized in the cytoplasm of avian sarcoma virus-transformed duck cells by viral DNA polymerase. Journal of Virology 18:567–573
    [Google Scholar]
  27. Varmus H. E., Guntaka R. V., Deng C. T., Bishop J. M. 1974; Synthesis, structure and function of avian sarcoma virus-specific DNA in permissive and nonpermissive cells. Cold Spring Harbor Symposia on Quantitative Biology 39:987–996
    [Google Scholar]
  28. Weinberg R. A. 1977; Structure of the intermediates leading to the integrated provirus. Biochimica et Biophysica Acta 473:39–55
    [Google Scholar]
  29. Woo S. L. C., Rosen J. M., Liarakos C. D., Choi Y. C., Busch H., Means A. R., O’Malley B. W. 1975; Physical and chemical characterization of purified ovalbumin messenger RNA. Journal of Biological Chemistry 250:7027–7039
    [Google Scholar]
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