1887

Abstract

Summary

Infection of cowpea protoplasts with alfalfa mosaic virus RNA was achieved by sedimenting protoplasts from 0.5 -mannitol and resuspending them in 0.7 -mannitol containing 0.1 -potassium phosphate, pH 6.0, and 100 µg/ml RNA. The osmotic shift-up employed during inoculation substantially enhanced the infection by virus RNA as well as by virus nucleoprotein.

As with intact plants, a mixture of the virus genomic RNA species (RNA 1, 2 and 3) was infectious to protoplasts only when supplemented either with virus coat protein or with the messenger RNA for coat protein (RNA 4).

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/content/journal/jgv/10.1099/0022-1317-41-3-653
1978-12-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-41-3-653
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